Navigation Links
Cells get sprayed
Date:1/18/2008

This release is also available in German.

Genetically engineered products have become indispensable. For example, genetically modified bacteria produce human insulin. In future, gene therapy should make it possible to introduce genes into the cells of a diseased organism so that they can address deficiencies to compensate for malfunctions in the body. In order for this to work, foreign (or synthetic) DNA must be introduced into host cells, which is not exactly a trivial task. Japanese researchers have now developed a method which could represent a true alternative to conventional processes. As described in the journal Angewandte Chemie, the cells are bombarded with water droplets produced and accelerated by electrospray.

There are several methods to transfer DNA into a host cell. In the simplest case the foreign DNA forces its way into the cell through a cell membrane that has been made porous, through treatment with electrical current or UV lasers, for example. Viruses and liposomes can be used as genetic transporters and the genetic material can be injected or shot into the cell with a particle gun. These methods all have the disadvantage of either severely damaging delicate cells or of being markedly expensive and complicated.

A team at the Saitama University led by Takafumi Sakai, in cooperation with Kazuto Ikemoto (Mitsubishi Gas Chemical Company), has now developed a methodology that could provide an alternative: They bombard the cells with tiny electrically charged water droplets. The droplets tear tiny holes in the cell membranes, through which external DNA molecules can enter. After about one minute, the holes have closed back up and even delicate cells survive the procedure undamaged.

This method is based on a technique called electrospray, which has long been used with success, particularly in mass spectrometry. In this process, th
'/>"/>

Contact: Takafumi Sakai
tsakai@mail.saitama-u.ac.jp
Wiley-Blackwell
Source:Eurekalert

Page: 1 2

Related biology news :

1. USC researchers identify mechanism that controls activation of stem cells during hair regeneration
2. Penn engineers create carbon nanopipettes that are smaller than cells and measure electric current
3. Type 1 diabetes triggered by lazy regulatory T-cells: McGill researchers
4. CSHL scientists identify cells that promote formation of lethal lung metastases
5. Breast cancer cells have to learn to walk before they can run
6. Lung cancer cells survival gene seen as drug target
7. Cornell researcher seeks clues to how tuberculosis infects cells
8. UC Irvine scientists find new way to sort stem cells
9. Bacteria that cause urinary tract infections invade bladder cells
10. CSHL scientists identify and repress breast cancer stem cells in mouse tissue
11. How molecular muscles help cells divide
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/29/2014)... Monday, September 29: Between 1970 and 2010 populations ... the globe dropped 52 percent, says the 2014 ... Fund (WWF). This biodiversity loss occurs disproportionately in ... of high-income countries. , In addition to the ... point to other warning signs about the overall ...
(Date:9/29/2014)... children at home should use ventilation when cooking with ... recommending, after a new study showed an association between ... chronic bronchitis. , "In homes where a gas stove ... wheezing is higher than in homes where a gas ... associate professor in the College of Public Health and ...
(Date:9/29/2014)... a bad rap, and understandably so. The grain-of-rice-sized insects ... millions of acres in the Western U.S. and Canada ... popular belief, these pests may not be to blame ... swept through the region. Instead, weather and topography play ... than these bark-boring beetles. , New research led by ...
Breaking Biology News(10 mins):Half of global wildlife lost, says new WWF report 2Childhood asthma linked to lack of ventilation for gas stoves, OSU study shows 2Childhood asthma linked to lack of ventilation for gas stoves, OSU study shows 3Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 2Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 3Tree killers, yes, fire starters, no: Mountain pine beetles get a bad rap, study says 4
... The advice and treatment given to patients with vertebral ... the Sahlgrenska Academy, University of Gothenburg, Sweden, shows that ... year after the fracture. Vertebral compression fracture ... spine such that its height is decreased. Approximately 15,000 ...
... Cambridge, MA --- The McGovern Institute for Brain Research ... Scolnick Prize in Neuroscience to Jeremy Nathans, a Howard ... and genetics, neuroscience, and opthalmology at Johns Hopkins University ... the Scolnick Prize lecture entitled "The Evolution of Trichromatic ...
... 23, 2009) The Wildlife Conservation Society announced today ... Africa are unusually resilient to climate change due to ... WCS announced the results of the study at ... this week in Phuket, Thailand. The study, published ...
Cached Biology News:Poor treatment for common vertebral compression fractures 2MIT: Jeremy Nathans to deliver Scolnick Prize lecture 2'Super reefs' fend off climate change, study says 2
(Date:9/30/2014)... (PRWEB) September 30, 2014 Proove ... medicine, is excited to announce a new genetic test ... The Proove Pain Perception Test will provide physicians ... and why pain tolerance levels are stratified between individuals. ... identify whether a person has a genetic predisposition that ...
(Date:9/30/2014)... 30, 2014  RegeneRx Biopharmaceuticals, Inc. (OTC: RGRX) ... has received a Canadian Patent for Inhibition or ... patent includes claims using thymosin beta 4, various ... for these purposes.  The patent will expire in ... ( www.regenerx.com ) RegeneRx is ...
(Date:9/30/2014)... Hungary , September 30, 2014 ... and intuitive browsing of chemistry in documents ... chemistry software solutions and consulting services for life ... chemical information and generate Markush structures from documents. ... an organizations intellectual property, however generating and understanding ...
(Date:9/30/2014)... 30, 2014 OriginOil Inc. (OTC/QB: OOIL), ... process to clean up large quantities of water, announced ... Eckelberry reported on the launch of the company's CLEAN-FRACâ„¢ ... provided by partner STW Resources . , Immediately ... per day (bpd) and achieved a 99.8% reduction in ...
Breaking Biology Technology:Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 2Proove Biosciences is Now Offering the Proove Pain Perception Genetic Test to Help Physicians Assess Patient Pain Tolerance Levels 3RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2ChemAxon Introduces ChemCurator for Chemical Information Extraction and Markush Structure Creation 2OriginOil, Inc. Report to Shareholders: Blue Gold in West Texas 2
... Inc. (NASDAQ: CYNO ) today announced financial results for ... for the first quarter of 2011 increased 16 percent to $21.9 ... Net loss for the first quarter of 2011 was $1.9 million, ... loss of $2.8 million, or $0.22 per basic and diluted share, ...
... 3, 2011 Answering the need for global, site-focused ... the patient enrollment industry, announces the expansion of its ... a local, site-centric level. "The extension of ... allow us to assist sponsors with their global patient ...
... MOUNTAIN VIEW, Calif., May 3, 2011 MAP Pharmaceuticals, Inc. ... the first quarter ended March 31, 2011. The ... was $17.6 million compared to a net loss of $14.1 ... the first quarter ended March 31, 2011 was related primarily ...
Cached Biology Technology:Cynosure Reports Financial Results for the First Quarter of 2011 2Cynosure Reports Financial Results for the First Quarter of 2011 3Cynosure Reports Financial Results for the First Quarter of 2011 4Cynosure Reports Financial Results for the First Quarter of 2011 5Cynosure Reports Financial Results for the First Quarter of 2011 6Clinical Site Services Expands to Provide Global Patient Enrollment Services to Rest-of-World 2MAP Pharmaceuticals Reports First Quarter of 2011 Financial Results 2MAP Pharmaceuticals Reports First Quarter of 2011 Financial Results 3MAP Pharmaceuticals Reports First Quarter of 2011 Financial Results 4MAP Pharmaceuticals Reports First Quarter of 2011 Financial Results 5
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Applications: Epitope mapping, Mapping protein-protein contacts, Identification of enzyme substrates/inhibitors, Identification of receptor agonists/antagonists...
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
... Quality ISO 9001:2000 Certified ... All Precedures Validated, SOP's Training ... Process for All Procedures GLP Documentation Upon ... one stop shopping point for all your ...
Biology Products: