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Celldance 2013 video awards, the 'Cell Oscars,' roll out tiny red carpet
Date:12/16/2013

his second place, Rakesh Suman, PhD, University of York in UK, created "Cellular Heaven and Hell," a time-lapse video obtained with the Phase Focus Virtual Lens 20(PFVL 20) microscope which uses ptychography to produce label free, high contrast and quantitative phase images. The healthy human gut epithelial cells in "heaven" were imaged under normal physiological conditions every 10 minutes and are played back at seven frames per second. The human gut epithelial cells that are in "hell" were treated with staurosporine to induce cell death, or apoptosis. Images were obtained every six minutes and are played back at seven frames per second

The other second place award went to Michael Schribak, PhD, Molecular Biology Laboratory, Woods Hole, MA. His move, a time-lapse video, shows the metaphase stage of meiosis in a crane fly (Nephrotoma suturalis) spermatocyte. Metaphase was captured by using a newly developed orientation-independent differential interference contrast technique. Each image in the movie represents 30 seconds, and the image acquisition and processing required about one second for each frame.

In Schribak's movie, the three autosomal bivalent chromosomes are in sharp focus at the spindle equator, along with one of the X-Y sex univalents, which is located on the right. The tubular distribution of mitochondria surrounding the spindle is clearly evident. Both polar flagella in the lower centrosome are in focus, appearing as a letter "L" lying on its side. Schribak conducted the experiment with James LaFountain, PhD, State University of New York, Buffalo, NY. The phase image was computed by David Biggs, PhD, KB Imaging Solution LLC, Loomis, CA.

In "The Cell Dance!" the winning entry in the "Public Outreach" category, Dartmouth researchers danced their favorite cellular processes. Pinar Gurel, graduate student, received the award and a cash prize of $250 for the cast of dancing biologists from the labs of Henry Higgs, PhD, Amy Gladf
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Contact: Cathy Yarbrough
cyarbrough@ascb.org
858-243-1814

John Fleischman
jfleischman@ascb.org
513-706-0212

American Society for Cell Biology
Source:Eurekalert


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