Tampa, Fla. (June 3, 2010) A team of researchers from several collaborating Baylor University research centers and from Japan's Okayama Graduate School of Medicine have found a way to more consistently isolate pancreatic islet cells from brain dead donors using ductal injection (DI), a process that immediately cools donor islet cells at the injection site. The more successful islet isolation process resulted in the three type 1-diabetes patients, who received islet cell transplants, becoming insulin independent.
Their study is published in issue 19(3) of Cell Transplantation and it is now freely available on-line at http://www.ingentaconnect.com/content/cog/ct/ .
"Inconsistent islet isolation is one of the important issues in clinical islet transplantation," said Dr. Shinichi Matsumoto, the research team's lead author. "Failure of donor islet isolation often results from the loss of the donor pancreas. Our simple modification of the retrieval process appears valuable for assuring greater success in islet transplantation."
Ductal injection is a procedure that modifies the islet isolation process using a cooling solution on the pancreatic islet cells derived from brain-dead donors. The cooling solution, applied at the donor's pancreatic ductal site, aids the viability of the islet cells.
The team successfully isolated islet cells in the DI group seven times while only three out of eight islet cell groups were isolated in the nonductal injection group. When islets from the DI group were transplanted into three type 1 diabetic patients, all three became insulin independent.
"DI significantly improved the quantity and quality of isolated islets and resulted in a high success rate of clinical islet transplantation," said Dr. Matsumoto.
According to the research team, a fifty percent success rate for clinical islet isolation has been standard; they w
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Cell Transplantation Center of Excellence for Aging and Brain Repair