Navigation Links
Case Western Reserve finds mechanism underlying alt. splicing of premessenger RNA into messenger RNA
Date:12/24/2008

Although it creates needed proteins, alternative splicing can also lead to problems. Mistakes in alternative splicing caused by changes (mutations) in DNA sequences create more than half of all genetic diseases. For instance, sometimes the resulting mRNA includes nucleotide sequences that should have been deleted.

Because the molecular mechanisms responsible for splice site choice remain poorly understood, Nilsen and his colleagues decided to study how some potential splice sites are "silenced," preventing them from forming splices while causing other, weaker splice sites to make splices instead. To do this they created a synthetic pre-mRNA containing two alternative splice sites, a weak site and a much stronger site downstream of it. Only one of them can be activated to make each possible mRNA, and whichever one the genetic machinery chooses, it then deletes a section of pre-mRNA from that beginning point to a common ending point downstream of both beginning splice points.

The research team randomized all possible combinations of nucleotides in a sequence of 12 nucleotides just downstream of the stronger splice site and in 12 just upstream of it, their exact positions chosen so that they would be as close as possible to the splice site without interfering with the very nearest nucleotides, which are required to complete the splices. This work was done both in vitro and in vivo, with similar results.

In control synthetic pre-mRNA's where the weak upstream splice site was left out, splicing always took place between the preferable downstream splice site and the common splice ending point. But when the weak upstream site was present, changes in nucleotide type and order near the downstream splicing site sometimes silenced it and sometimes did not.

Using bioinformatics techniques, the researchers organized the 89 unique intronic silencer sequences they found into four distinct clusters, while the 47 unique exonic silencers formed
'/>"/>

Contact: Christina DeAngelis
christina.a.thompson@case.edu
216-368-3635
Case Western Reserve University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Western diet linked to increased risk of colon cancer recurrence
2. UT Southwestern researchers identify hundreds of genes controlling female fertility
3. Northwestern to expose most deadly infectious diseases in 3-D
4. Northwestern study looks at sensing, movement and behavior
5. Tiny dust particles from Asian deserts common over western United States
6. Threatened bird species finds home at Western
7. BMI criteria for obesity surgery should be lowered, UT Southwestern researcher suggests
8. UT Southwestern secures $5 million NIH grant for lupus research
9. UT Southwestern scientist honored among best in Texas research
10. Western Groundfish Conference to be held in Santa Cruz on Feb. 4-8
11. Stem-cell transplantation improves muscles in MD animal model, UT Southwestern researchers report
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:10/16/2014)... are anything but sustainable: environmental damage to soil ... increasingly evident. Despite their disadvantages, however, monocultures remain ... the sole possibility of achieving higher yields in ... an ecology professor at the University of Zurich, ... forestry. After all, a new study carried out ...
(Date:10/15/2014)... N.Y. , Oct. 15, 2014 ... solutions for home and community-based care, today announced ... of implementing Sandata,s Santrax® Electronic Visit Verification™ Solution ... Services is a home health company founded in ... Texas . The study ...
(Date:10/15/2014)... The Ebola virus is spreading rapidly and to an ... experienced in the past and the virus shows a ... been recorded before. For this reason, the German National ... of Science and Engineering, and the Union of the ... statement on the Ebola epidemic today. , In the ...
Breaking Biology News(10 mins):Plant communities produce greater yield than monocultures 2Plant communities produce greater yield than monocultures 3Sandata Announces Case Study with Quality Care Services, Inc. 2Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5
... each living cell needs both to build new and ... a number of intracellular systems work in concert to ... damaged proteins. When proteins or peptides mutate, they can ... intracellular environment. In Huntington's disease (HD) the disease provoking ...
... Wildlife Conservation Society working in Iran has successfully fitted ... marking the first time this highly endangered population of ... found throughout the continent, Asiatic cheetahs now live only ... Kavir Desert. WCS's government partner in Iran, the Department ...
... An international group of scientists has announced a ... describe the activities of genes in living organisms. ... use to describe the complex events that occur ... host. Understanding these events is crucial for developing ...
Cached Biology News:Proteasome activator enhances survival of Huntington's disease neuronal model cells 2In Iran, cheetahs collared for the first time 2Uniform language for describing genes of pathogenic and beneficial microbes 2Uniform language for describing genes of pathogenic and beneficial microbes 3
(Date:10/20/2014)... , Oct. 20, 2014  GenVec, Inc. (NASDAQ: ... Horovitz , Ph.D., from its board of directors effective on ... August 2003, and served as its chairman from June 2006 ... the Nominating and Corporate Governance and Audit Committees of the ... than a decade of dedicated service to GenVec, and its ...
(Date:10/20/2014)... 20, 2014 OncLive® ... Cancer Center at Thomas Jefferson University has joined ... the Strategic Alliance Partnership program, the Sidney Kimmel ... to raise awareness of the Center’s cutting-edge research ... projects. Clinicians and other health care professionals from ...
(Date:10/20/2014)... Local veterinary surgeon, Dr. Tim McCarthy is ... donor stem cells for dogs with osteoarthritis. Dr. McCarthy ... performed clinical stem cell therapy for 7 years. The ... a single injection of donor stem cells into one ... and inflammation in the treated joints. , Candidates ...
(Date:10/19/2014)... 20, 2014 OCTOBER 20-22, ... (ABIM). ABIM will take place at ... about ABIM 2014 is now available at ... representing companies and organizations from all over ... information on the latest products and developments ...
Breaking Biology Technology:Zola P. Horovitz To Retire From GenVec Board 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 2Sidney Kimmel Cancer Center at Thomas Jefferson University Partners With OncLive 3Cascade Veterinary Referral Center Seeks Candidates for an Investigational Study of Stem Cells for Dogs with Arthritis 2
... Norlight Telecommunications , a Brookfield, Wisconsin, ... Protocol service for small and medium-sized businesses in Wisconsin ... to offer the service to businesses in Minnesota, Michigan, ... marketing. , , IPLive will integrate voice, data ...
... Wisconsin-based Analogix joins French company to provide drug purification ... businesses Alliant Energy announces dip in ... furniture store selects Citrix IT tools ... purification products , Analogix , a maker of ...
... courtesy of Silicon Graphics Inc. Chippewa Valley, Wis. ... fastest supercomputer in about 129 days, and it was the ... SGI announced the completion of the $50 million project for ... after the space shuttle that crashed last year, runs on ...
Cached Biology Technology:Tech Digest: whats moving in the Midwest (11/1/04) 2SGIs Wisconsin branch builds fastest supercomputer yet in four months 2
Yields DNA greater than 50 kb in length from yeast, fungi, Gram neg. and Gram pos. bacteria...
Mouse monoclonal [1Q17] to TGE Virus peplomer ( Abpromise for all tested applications)....
Jak3 Antibody Ship: Hot Store: -20 C...
14-3-3 tau Antibody Shipping Temperature: HOT Storage Temperature: -20C...
Biology Products: