Navigation Links
Carnegie donates landmark clones to biology
Date:8/6/2009

Palo Alto, CAWith the information explosion, it's remarkable that so little is known about the interactions that proteins have with each other and the protective membrane that surrounds a cell. These interactive, so-called membrane proteins regulate nutrients and water fluxes, sense environmental threats, and are the communications interface with neighboring cells and within the cell. Now with National Science Foundation funding, researchers at the Carnegie Institution's Department of Plant Biology have cloned genes to produce membrane proteins that may initiate the instructions for genes to turn on in the nucleus. They just donated 2010 of the clones for genes that function in the cell's interaction with its environment to the Arabidopsis Biological Resource Center (ABRC is at Ohio State University) for other scientists to use to help advance fields from medicine to farming. These genes are now used to unravel the interaction of the membrane proteins amongst each other.

Recent research at the Carnegie department has shown that cells across different species use the same mechanism at the cell membrane to regulate the uptake of the vital nutrient nitrogen. Previous Carnegie work indicated that plants have a novel regulatory mechanism that controls nutrient uptakeneighboring pore-like structures at a plant cell's surface physically interact to control the uptake. "Since plants, animals, bacteria, and fungi all share similar genes for this activity, we wanted to see in this study if same feature could occur across species," remarked Dominique Loqu lead author of a study published in the July 6, Journal of Biological Chemistry.

In the previous work, the scientists looked at the end of the protein Arabidopsis ammonium transporter (AMT1;1). This protein portion is called the C-terminus and it regulates the interactions of the pore-like structures at the membrane surface in plants. In this study they focused on the underlying mechanism of the pore activity by using mutant proteins that cannot shut the pores off with their C-terminus to see how they work in yeast and immature eggs of the frog Xenopus in the presence of ammonium.

The researchers were totally surprised that the mechanism in which three subunits regulate each other was found in the primitive archaebacteria. It means that it evolved billions of years ago. The fact that the C-terminus is found in all other bacteria, fungi, and plants demonstrates that it was necessary in the atmosphere where they developedperiods in which the toxic ammonium accumulated on the early Earth. This mechanism has been retained although a single mutation can make the transporters work independently. So why did this simpler mutation not succeed? The researchers believe that there must still be selective pressure on the system. The simplest explanation is that the mechanism is still necessary today, probably to control uptake and prevent toxicity.

"The newly donated 2010 clones will now be used to see how common such regulation by neighbors is. It also emphasizes the importance and the potential that the new clones have for understanding a spectrum of problems from kidney diseases to engineering better crops," remarked director of the department Wolf Frommer.


'/>"/>

Contact: Wolf Frommer
wfrommer@ciw.edu
650-325-1521
Carnegie Institution
Source:Eurekalert

Related biology news :

1. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
2. Carnegie Mellon scientists investigate initial molecular mechanism that triggers neuronal firing
3. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
4. Carnegie Mellon, Pitt Team to study psychosocial stress
5. Mellon awards Carnegie Grant for Ecological Monitoring in South Africa
6. Carnegie Mellon researchers to develop new drug delivery system
7. Carnegie Mellon students win contest
8. Novel mechanism for long-term learning identified by Carnegie Mellon researchers
9. U. Mass Medical School and Carnegie announce licensing agreements with Oxford BioMedica
10. Carnegie Mellon scientists develop fluorescent proteins for live cell imaging, biosensor design
11. Carnegie Mellon receives $1.85 million
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:6/16/2016)... June 16, 2016 The ... expected to reach USD 1.83 billion by 2024, ... Research, Inc. Technological proliferation and increasing demand in ... expected to drive the market growth. ... The development of advanced multimodal techniques for ...
(Date:6/3/2016)... 3, 2016 Das ... Nepal hat ein 44 ... geprägter Kennzeichen, einschließlich Personalisierung, Registrierung und IT-Infrastruktur, ... Produktion und Implementierung von Identitätsmanagementlösungen. Zahlreiche renommierte ... Januar teilgenommen, aber Decatur wurde als konformste ...
(Date:5/24/2016)... 24, 2016 Ampronix facilitates superior patient care by providing unparalleled technology to ... display is the latest premium product recently added to the range of products distributed ... ... ... Imaging- LCD Medical Display- Ampronix News ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... Mass. , June 23, 2016   ... development of novel compounds designed to target cancer ... napabucasin, has been granted Orphan Drug Designation from ... the treatment of gastric cancer, including gastroesophageal junction ... stemness inhibitor designed to inhibit cancer stemness pathways ...
(Date:6/23/2016)... -- Houston Methodist Willowbrook Hospital has signed a ... serve as their official health care provider. As ... provide sponsorship support, athletic training services, and most ... athletes and families. "We are excited ... to bring Houston Methodist quality services and programs ...
(Date:6/23/2016)... YORK , June, 23, 2016  The Biodesign ... to envision new ways to harness living systems and ... Modern Art (MoMA) in New York City ... than 130 participating students, showcased projects at MoMA,s Celeste ... Paola Antonelli , MoMA,s senior curator of architecture ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... STACS DNA ... Technical Leader at the Arkansas State Crime Laboratory, has joined STACS DNA as a ... STACS DNA team,” said Jocelyn Tremblay, President and COO of STACS DNA. “In further ...
Breaking Biology Technology: