Navigation Links
Carnegie Mellon scientists develop fluorescent proteins for live cell imaging, biosensor design
Date:2/6/2008

PITTSBURGH Scientists at Carnegie Mellon Universitys Molecular Biosensor and Imaging Center have developed new fluorogen activating proteins (FAPs) that will become a key component of novel molecular biosensor technology being created at Carnegie Mellon. The FAPs, which can be used to monitor biological activities of individual proteins and other biomolecules within living cells in real time, are described in the February issue of Nature Biotechnology.

Carnegie Mellon scientists designed the FAPs to emit fluorescent light only when bound to a fluorogen, an otherwise non-fluorescent dye added by the scientists. This feature will allow biologists to track proteins on the cell surface and within living cells in very simple and direct ways, eliminating cumbersome experimental steps.

Scientists say the fluorogen activating proteins are especially useful for developing molecular biosensors, because FAPs allow researchers to not only see where the target protein is within the space of the cell, but also to see color changes when it becomes fluorescent. Color changes may reflect changes in the local environment of the protein, and allow quantitative sensing in real time of the biological activity of proteins and biomolecules that are in close proximity to each other.

Biologists often have a difficult time locating a target biomolecule inside living cells using other dye technologies because of background light given off by any unbound dye molecules. This background light obscures the biomolecules glow and therefore must be removed to successfully carry out the experiment.

The new FAP technology gives off light only when and precisely where the target biomolecule is present, enabling scientists to activate the fluorescence when needed to see exactly where in the cell the biomolecule is located. Scientists also can design fluorogens that can enter the cell and fluorogens that cant. When used with fluorogens that are excluded from th
'/>"/>

Contact: Jocelyn Duffy
jhduffy@andrew.cmu.edu
412-268-9982
Carnegie Mellon University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
2. Carnegie Mellon scientists investigate initial molecular mechanism that triggers neuronal firing
3. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
4. Carnegie Mellon, Pitt Team to study psychosocial stress
5. Mellon awards Carnegie Grant for Ecological Monitoring in South Africa
6. Carnegie Mellon researchers to develop new drug delivery system
7. Carnegie Mellon students win contest
8. Novel mechanism for long-term learning identified by Carnegie Mellon researchers
9. U. Mass Medical School and Carnegie announce licensing agreements with Oxford BioMedica
10. UK scientists working to help cut ID theft
11. Scientists show that mitochondrial DNA variants are linked to risk factors for type 2 diabetes
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/12/2015)... , Aug. 12, 2015  New research unveiled ... attack Android phones to steal users, fingerprints. Information ... availability of fingerprint scanners on mobile devices, making ... To secure biometrics on mobile devices, HYPR Corp. ... these convenient authentication systems with strong cryptographic security. ...
(Date:8/10/2015)... LONDON , August 10, 2015 ... visiongain offers comprehensive analysis of the global border ... will generate revenues of $16.4bn in 2015. Now: ... This is an example of the business critical issue you ... to read visiongain ,s objective analysis of how this ...
(Date:8/5/2015)... CHICAGO , August 5, 2015 ... Identity & Access Management Market by Software, Services, Vertical (BFSI, ... Chemical/Pharma), & Geography - Global Forecast to 2020", published ... Access Management Market globally into various segments. The global ... in 2014 to $546.2 Million by 2019, at a ...
Breaking Biology News(10 mins):HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 2HYPR Corp. Addresses Android Vulnerability That Exposes Ecosystem-wide Biometric Security Challenges 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 2Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 3Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 4Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 5Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 6Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 7Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 8Border Security Market Report 2015-2025 - Growth in Biometrics, ICT, Manned and Unmanned Vehicles, and Perimeter Surveillance 9Physical Identity and Access Management Market Worth $546.2 Million by 2019 2Physical Identity and Access Management Market Worth $546.2 Million by 2019 3Physical Identity and Access Management Market Worth $546.2 Million by 2019 4
... options and pressures influence nearly every part of children,s ... a special supplement of the American Journal of Preventative ... at the University of Michigan, reveal that, in most ... soft drink bottling companies give students easy access to ...
... is one of two gene-hunting facilities in the nation ... to rigorously sort out how such environmental factors as ... genetic makeup to affect their risk for disorders as ... The Johns Hopkins Center for Inherited Disease ...
... HARBOR, N.Y. -- The first manual specifically geared ... Cold Spring Harbor Laboratory Press ( www.cshlpress.com ). ... from bioinformatics, technology, statistics, and biology, to provide a ... that are feasible for a wide range of laboratories. ...
Cached Biology News:Studies: Children obese due to a host of unhealthy pressures 2Studies: Children obese due to a host of unhealthy pressures 3Studies: Children obese due to a host of unhealthy pressures 4'Genes and environment' grant funds close look at nature-nurture overlap in common diseases 2'Genes and environment' grant funds close look at nature-nurture overlap in common diseases 3New book presents successful strategies for probing genetic variation 2New book presents successful strategies for probing genetic variation 3
(Date:9/2/2015)... , Sept. 2, 2015  Neurotech Pharmaceuticals, Inc., ... in the multicenter Phase 2 clinical trial of ... treatment of recurrent subfoveal choroidal neovascularization secondary to ... a unique vascular endothelial growth factor (VEGF) receptor ... "This landmark proof-of-concept study will evaluate ...
(Date:9/2/2015)... , Sept. 2, 2015 Aytu BioScience, ... developing treatments for urological and related conditions, announced today ... planned private placement convertible note financing, raising a total ... tranches totaling $3.175 million. Josh ... from this private placement are intended to be used ...
(Date:9/2/2015)... , September 2, 2015 ... BOLT) ("BioLight" or the "Company"), a firm that ... ophthalmology and cancer diagnostics, announced today that presentations ... glaucoma will be delivered at the Ophthalmology Futures ... European Society of Cataract & Refractive Surgeons ("ESCRS"), ...
(Date:9/2/2015)... 2015 Biovista Inc. freut ... von 2015 bekanntgeben zu können. Hierbei handelt es ... Biovista unterstützt mit seiner Möglichkeit der ... anderer Krankheiten als denen, für die ein Medikament ... sein Team bei der Wylder Nation Foundation bei ...
Breaking Biology Technology:Neurotech Announces First Patient Enrolled in Novel Anti-VEGF Encapsulated Cell Therapy Study 2Neurotech Announces First Patient Enrolled in Novel Anti-VEGF Encapsulated Cell Therapy Study 3Neurotech Announces First Patient Enrolled in Novel Anti-VEGF Encapsulated Cell Therapy Study 4Aytu BioScience Completes $5.175 Million Private Placement 2Aytu BioScience Completes $5.175 Million Private Placement 3Presentations to Highlight BioLight's IOPtiMate[TM] System at Upcoming European Ophthalmology Conferences 2Presentations to Highlight BioLight's IOPtiMate[TM] System at Upcoming European Ophthalmology Conferences 3Biovista sponsert die Forschung von Wylder Nation Foundation zum Thema Mangel an saurer Sphingomyelinase 2
... Hanover Insurance Group, Inc. (NYSE: THG ... insurance products nationwide, announced today that it has ... equipment, orthotics and prosthetics sector -- which are ... (Logo: http://photos.prnewswire.com/prnh/20051031/NEM023LOGO) The new ...
... Tengion, Inc (NASDAQ: TNGN ), a leader in ... Chief Financial Officer of Tengion, will present at the upcoming ... 13, 2011, at 1:35 pm EDT. A ... of Events" in the Investors section of the Company,s website ...
... Sept. 7, 2011 MedWaves, AveCure™ microwave coagulation-ablation ... (destroy) soft-tissue lesions (abnormal structures) safely, effectively, precisely ... over one-thousand patients worldwide, including lesions in bone, ... to bring its technology to treat lesions in ...
Cached Biology Technology:The Hanover Expands Innovative Solution for the Durable Medical Equipment, Orthotics and Prosthetics Market 2The Hanover Expands Innovative Solution for the Durable Medical Equipment, Orthotics and Prosthetics Market 3Tengion to Present at Rodman & Renshaw 13th Annual Healthcare Conference 2MedWaves, Incorporated Announces Receipt of the Chinese State Food and Drug Administration (SFDA) Certification to Import and Sell Its Patented AveCure™ Microwave Ablation System and Devices 2MedWaves, Incorporated Announces Receipt of the Chinese State Food and Drug Administration (SFDA) Certification to Import and Sell Its Patented AveCure™ Microwave Ablation System and Devices 3
... The pACHLT-A-BFP Baculovirus Transfer Vector is a derivative ... the gene for blue fluorescent protein (BFP), followed ... site (MCS).,Foreign genes are expressed as BFP-6xHis fusion ... restriction enzyme,sites (EcoR I, Stu I, Sac I, ...
Mouse monoclonal antibody to PDK2 - pyruvate dehydrogenase kinase, isoenzyme 2...
... pLivSelect is a direct antibiotic-based selection ... solely on colony survival, eliminating the need ... inexpensive process provides close to 100% accuracy ... ideal platform for robot colony picking. This ...
Mouse monoclonal to Casein Kinase 2 beta ( Abpromise for all tested applications). entrezGeneID: 1460 SwissProtID: P13862...
Biology Products: