Navigation Links
Carnegie Mellon researchers develop artificial cells to study molecular crowding and gene expression
Date:7/14/2013

PITTSBURGHThe interior of a living cell is a crowded place, with proteins and other macromolecules packed tightly together. A team of scientists at Carnegie Mellon University has approximated this molecular crowding in an artificial cellular system and found that tight quarters help the process of gene expression, especially when other conditions are less than ideal.

As the researchers report in an advance online publication by the journal Nature Nanotechnology, these findings may help explain how cells have adapted to the phenomenon of molecular crowding, which has been preserved through evolution. And this understanding may guide synthetic biologists as they develop artificial cells that might someday be used for drug delivery, biofuel production and biosensors.

"These are baby steps we're taking in learning how to make artificial cells," said Cheemeng Tan, a Lane Postdoctoral Fellow and a Branco-Weiss Fellow in the Lane Center for Computational Biology, who led the study. Most studies of synthetic biological systems today employ solution-based chemistry, which does not involve molecular crowding. The findings of the CMU study and the lessons of evolution suggest that bioengineers will need to build crowding into artificial cells if synthetic genetic circuits are to function as they would in real cells.

The research team, which included Russell Schwartz, professor of biological sciences; Philip LeDuc, professor of mechanical engineering and biological sciences; Marcel Bruchez, professor of chemistry; and Saumya Saurabh, a Ph.D. student in chemistry, developed their artificial cellular system using molecular components from bacteriophage T7, a virus that infects bacteria that is often used as a model in synthetic biology.

To mimic the crowded intracellular environment, the researchers used various amounts of inert polymers to gauge the effects of different density levels.

Crowding in a cell isn't so differen
'/>"/>

Contact: Byron Spice
bspice@cs.cmu.edu
412-268-9068
Carnegie Mellon University
Source:Eurekalert

Page: 1 2

Related biology news :

1. Keck award enables Carnegie Mellon and Stanford to dramatically expand crowdsourced RNA design
2. Carnegies Greg Asner named Energy/Climate Fellow by US State Department
3. Carnegie Mellon fluorescent biosensor reveals mechanism critical to immune system amplification
4. Carnegies Wolf B. Frommer receives Bogorad Award for Excellence in Plant Biology
5. Carnegie Mellon Universitys Biometrics Center Selected To House New Pedo-Biometrics Research and Identity Automation Lab
6. Carnegie Mellon study shows skin-aging radicals age naturally formed particles in the air
7. Carnegies Donald Brown wins Lasker-Koshland Award
8. Carnegie Institution for Science receives Grand Challenges Explorations grant
9. Carnegie debuts revolutionary biosphere mapping capability at AGU
10. Carnegies Greg Asner elected to National Academy of Sciences
11. Carnegie Mellon neuroscientists discover new phase of synaptic development
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/10/2014)... DUBLIN , Dec. 08, 2014 Research ... announced the addition of the "Biometrics Market ... http://photos.prnewswire.com/prnh/20130307/600769 ... for projects such as rural banking and upgradation ... major trends witnessed in the market. Besides the ...
(Date:12/5/2014)... 4, 2014 Increased emphasis on product ... new testing and inspection technologies. The convergence of ... a range of innovative test solutions. This trend ... Y, which is generally more inclined towards communication, ... development strategies of test equipment vendors will cater ...
(Date:12/4/2014)... 3, 2014  Crossmatch™, a leading provider of ... DigitalPersona ® Pro Enterprise software and U.are.U ... throughout First Bank branch locations in ... and Virginia. First Bank, a progressive community ... North Carolina , selected the Crossmatch biometrics-based ...
Breaking Biology News(10 mins):Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 3Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 4First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3
... brain, but they also leave behind in the bloodstream a ... the future to direct better treatment for schizophrenia and other ... study is among the first to use metabolomics -- the ... processes -- to look at a psychiatric disease and its ...
... Research Institute on Addictions (RIA) is investigating the “brain’s ... stress, stress-related behavior and anxiety. , A five-year, $1.7 ... is supporting this investigation. , “It is widely ... use and abuse marijuana is to relieve stress,?according to ...
... generators (dopaminergics) in brain cells has produced improvement in ... tests carried out with monkeys by the Navarra University ... at the Hospital and at the other Navarra University-based ... , The results have been published in the latest ...
Cached Biology News:Chemical maps hint at drug's effects on schizophrenia 2Chemical maps hint at drug's effects on schizophrenia 3Implanting dopamine generators in brain cells obtains improvement in Parkinson's in monkeys 2Implanting dopamine generators in brain cells obtains improvement in Parkinson's in monkeys 3
(Date:12/24/2014)... , Dec. 23, 2014 Vermillion, ... focused on gynecologic disease, announced today that investors ... , Birchview Fund LLC and several Vermillion directors ... unregistered shares of Vermillion,s common stock and warrants ... in a private placement.  Under ...
(Date:12/24/2014)... 2014  Rock Creek Pharmaceuticals, Inc., (NASDAQ: ... Clinical Trial Application (CTA) with the United ... seeking regulatory approval to initiate clinical trials for the ... Contingent on the Company receiving CTA regulatory approval, ... to assess the safety, tolerability and dose escalation of ...
(Date:12/22/2014)... 2014   Synthetic Biologics, Inc. ... therapies for serious infections and diseases, with ... announced positive topline safety and tolerability results ... SYN-004, the Company,s investigational oral beta-lactamase enzyme ... (C. difficile) infection, antibiotic-associated diarrhea and ...
(Date:12/22/2014)... , Dec. 22, 2014 GenomeDx Biosciences ... Decipher Prostate Cancer Classifier, a genomic test for prostate ... node-negative, high-risk men managed by radical prostatectomy without adjuvant ... prostatectomy is relatively uncommon, using tumor genomics to identify ... metastatic disease is an important advance. The study has ...
Breaking Biology Technology:Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 3Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 4Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 5Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 2Rock Creek Pharmaceuticals Files Clinical Trial Application in the UK for Clinical Trials of Anatabine Citrate Formulations 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 2Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 3Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 4Synthetic Biologics Announces Positive Topline Results from Phase 1a Trial of SYN-004 for the Prevention of C. difficile Infection 5Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 2Study Published in European Urology Shows that the Decipher Prostate Cancer Classifier is Predictive of Rapid Metastasis in High-Risk Men who have had Prostate Surgery 3
... BioMS Medical Corp (TSX: MS), a leading developer in the ... Stroud, Vice President Regulatory Affairs, will present at the Canadian ... TITLE: "Dirucotide: ... Thursday June 4th ...
... of integrating and sustaining positive change, increasing wellnessWASHINGTON, ... stress can wreak havoc on a body,s systems. ... change paradigm helps individuals cultivate greater wellness, make ... of life in the face of challenges.The approach, ...
... presenting at the Global Crop Protection Summit and exposition on 15-17th ... ... London, UK (PRWeb UK) - Pira International and ... Protection Summit in Edinburgh, Scotland on 15 - 17 September 2009. ...
Cached Biology Technology:BioMS Medical to present at the 2009 Symposium for the Canadian Society for Pharmaceutical Sciences 2Reorganizational Healing Provides Access to Greater Quality of Life, Reduced Stress 2Call for papers for Pira International's Global Crop Protection Summit 2009 2
... Security ChemStation is a ... application for data acquisition, ... searching and reporting, addressing ... data security, integrity, and ...
... These handy dispensers save ... glasses, wipers, hats, and other ... benchtop storage bins for small ... and manufacturing. Clear acrylic. Open-slot ...
... for genome-wide microRNA expression profiling and ... Paraflo microfluidic on-chip synthesis platform. These ... our comprehensive microRNA Expression Profiling Service. ... Soil Nematode microRNA Microarray contains all ...
... is critical to the maintenance of ... proteins as important and diverse as ... shock and stress response, antigen presentation, ... ion channels, cell cycle regulation, transcription, ...
Biology Products: