Navigation Links
Carnegie Mellon MRI technology that non-invasively locates, quantifies specific cells in the body
Date:8/20/2008

PHILADELPHIAMagnetic resonance imaging (MRI) isn't just for capturing detailed images of the body's anatomy. Thanks to novel imaging reagents and technology developed by Carnegie Mellon University scientist Eric Ahrens, MRI can be used to visualize with "exquisite" specificity cell populations of interest in the living body. The ability to non-invasively locate and track cells, such as immune cells, will greatly aid the study and treatment of cancer, inflammation, and autoimmune diseases, as well as provide a tool for advancing clinical translation of the emerging field of cellular regenerative medicine, by tracking stem cells for example.

Ahrens will present his research on this new approach, called fluorocarbon labeling, Thursday, Aug. 21 at the 236th national meeting of the American Chemical Society in Philadelphia.

"With our technology we can image specific cells in real-time with exquisite selectivity, which allows us to track their location and movement and to count the apparent number of cells present. We then use conventional MRI to obtain a high-resolution image that places the labeled cells in their anatomical context," said Ahrens, an associate professor of biological sciences at the Mellon College of Science.

The ability to track the movement and eventual location of specific immune cells is critical for understanding the cells' role in disease and therapeutic mechanisms, and for developing effective cell-based therapeutics. Other MRI methods for visualizing cells use metal-based contrast agents, which can make it difficult to clearly identify labeled cells in the body, according to Ahrens.

"The large background signal from mobile water and intrinsic tissue contrast differences can often make it challenging to unambiguously identify regions containing these metal-ion labeled cells throughout the body, which is the current state of the art," Ahrens said.

Ahrens's new approach fluorocarbon labeling solves this problem by producing images that clearly show the labeled cells at their precise location in the body. Ahrens first labels the cells of interest with a perfluoropolyether (PFPE) nanoemulsion, which is a colloidal suspension of tiny fluorocarbon droplets. Then, he introduces the labeled cells into an animal subject and tracks the cells in vivo using 19F MRI.

While conventional MRI detects the nuclear magnetic resonance signal from protons contained in the mobile water in tissue, 19F MRI detects the signal from the nucleus of the fluorine atom. Fluorine is not normally present in the body at sufficient concentrations to detect, so when Ahrens labels cells with PFPE, he can detect this fluorine 'tracer' with MRI after the cells are transplanted into the body. The Ahrens' team has recently used the PFPE technology to label and track dendritic cells and T cells in a mouse model of type I diabetes, a disease in which immune cells infiltrate the pancreas, attacking and damaging the body's own cells.

"Right now we're using our technology to image key cell types involved in autoimmune diseases like type I diabetes, but our cellular MRI agents also can be adapted to label other cell types, including cells from bone marrow and stem cells. A key long-term application of our technology is to label and monitor cell-based therapeutics in humans," Ahrens said.

Recent advances in cell-based therapeutics research have focused on training immune cells to counteract diseases including cancer and diabetes and on directing stem cells to regenerate damaged tissues. Non-invasively visualizing these therapeutic cells in patients after transfer can be a vexing problem, according to Ahrens, and any approach that can speed up the testing of these treatments will be extremely useful.

"Ideally we would label therapeutic cells with our cellular MRI agents before they are implanted into a patient. In this way, we could use MRI to visualize the movement of the therapeutic cells in the patient to monitor whether they migrate to and remain in the desired tissues," explained Ahrens.


'/>"/>
Contact: Jocelyn Duffy
jhduffy@andrew.cmu.edu
412-268-9982
Carnegie Mellon University
Source:Eurekalert

Related biology news :

1. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
2. Carnegie Mellon scientists investigate initial molecular mechanism that triggers neuronal firing
3. Carnegie Mellon scientist uses mass spectrometer to weigh virus particle, von Willebrand factor
4. Carnegie Mellon, Pitt Team to study psychosocial stress
5. Mellon awards Carnegie Grant for Ecological Monitoring in South Africa
6. Carnegie Mellon researchers to develop new drug delivery system
7. Carnegie Mellon students win contest
8. Novel mechanism for long-term learning identified by Carnegie Mellon researchers
9. U. Mass Medical School and Carnegie announce licensing agreements with Oxford BioMedica
10. Carnegie Mellon scientists develop fluorescent proteins for live cell imaging, biosensor design
11. Carnegie Mellon receives $1.85 million
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:5/16/2016)... May 16, 2016   EyeLock LLC , a ... the opening of an IoT Center of Excellence in ... expand the development of embedded iris biometric applications. ... of convenience and security with unmatched biometric accuracy, making ... aside from DNA. EyeLock,s platform uses video technology to ...
(Date:5/3/2016)... 2016  Neurotechnology, a provider of high-precision biometric ... Biometric Identification System (ABIS) , a complete system ... ABIS can process multiple complex biometric transactions with ... fingerprint, face or iris biometrics. It leverages the ... MegaMatcher Accelerator , which have been used ...
(Date:4/26/2016)... BANGALORE, India and LONDON ... Infosys Finacle, part of EdgeVerve Systems, a ... ), and Onegini today announced a partnership to ... banking solutions.      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ... banks to provide their customers enhanced security to ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... ... June 24, 2016 , ... Researchers at the Universita Politecnica delle ... people with peritoneal or pleural mesothelioma. Their findings are the subject of a new ... , Diagnostic biomarkers are signposts in the blood, lung fluid or tissue of mesothelioma ...
(Date:6/23/2016)...   Boston Biomedical , an industry leader ... target cancer stemness pathways, announced that its lead ... Designation from the U.S. Food and Drug Administration ... gastroesophageal junction (GEJ) cancer. Napabucasin is an orally ... stemness pathways by targeting STAT3, and is currently ...
(Date:6/23/2016)... HOUSTON , June 23, 2016 ... agreement with the Cy-Fair Sports Association to serve ... of the agreement, Houston Methodist Willowbrook will provide ... education and connectivity with association coaches, volunteers, athletes ... partner with the Cy-Fair Sports Association and to ...
(Date:6/23/2016)... 23, 2016  The Biodesign Challenge (BDC), a university ... to harness living systems and biotechnology, announced its winning ... New York City . ... showcased projects at MoMA,s Celeste Bartos Theater during the ... MoMA,s senior curator of architecture and design, and ...
Breaking Biology Technology: