Navigation Links
Cantilever sensory array: The Rosetta Stone for antibiotic resistance?
Date:10/25/2013

On October 25, JoVE, the Journal of Visualized Experiments will publish a novel technique to confront the problem of antibiotic resistance. According to Dr. Joseph Ndieyira, one of the developers involved in the technique, "The use of this technology will allow scientists to understand how antibiotics work, how bacteria develop resistance, and what molecular mechanisms could be exploited to get around their defense mechanisms."

"We report a novel, nanomechanical approach to investigate the workings of vancomycin ... one of the last powerful antibiotics used to combat increasingly-resistant infections such as methicillin-resistant Staphylococcus aureus (MRSA)," said Ndieyira.

Using tiny cantilevers (or beams) no wider then a human hair, Ndieyira and his colleagues take advantage of the cellular stress that antibiotics, when effective, impose on a target bacteria's cell wall. Such stress in turn causes the cantilevers to bend. Using lasers, the bending can then be measuredproviding tremendous insight into the drug-target interaction.

According to Ndieyira, the cantilever technology confronts a growing problem of multidrug-resistant hospital superbugs. It could aid in the drug discovery process by preventing lead-drug candidates from being disregarded due to a lack of equipment with sufficient sensitivity, and its hope is to jumpstart an already stagnated drug-pipeline by providing this sensitivity in an efficient mannerthe cantilever method can simultaneously measure and track a variety of drug-bacteria interactions in real time.

"The cantilever assays provide a resolution that simply cannot be obtained with conventional methods, such as those using fluorescence," said Ndieyira, "For example, cantilever sensors can resolve forces of ~10 pN, which is sensitive enough to detect the rupturing of individual hydrogen bonds." According to the article, a single hydrogen molecule can be the difference between drug-susceptible and drug-resistant bacteria.

The cantilever technique is currently under review in the science journal Nature for its clinical use in a microchip. "This prototype will provide a platform for more sensitive measurements, enabling results in early detection of infectious diseases, rapid identification of a host of new biomarkers, and for the engineering of portable diagnostic tools," said Ndieyira. He also described the technique as a possible alternative for drug experimentation on animals.

When asked why he and his colleagues decided to publish their technique through video, Ndieyira said, "JoVE is the only journal that provides a unique and powerful opportunity to communicate my latest measurements and procedures visually, thus making it easy and effective for others to replicate the experiment."


'/>"/>

Contact: Rachel Greene
rachel.greene@jove.com
617-250-8451
The Journal of Visualized Experiments
Source:Eurekalert  

Related biology news :

1. Architecture of rod sensory cilium disrupted by mutation
2. Scientists discover a new sensory organ in the chin of baleen whales
3. A whale of a discovery: New sensory organ found in rorqual whales
4. South African living stone plant adapts to extreme conditions in new ways
5. UdeMs IRIC and IRICoR achieve important milestone in their collaboration with Bristol-Myers Squibb
6. DO-HEALTH a multi-center clinical trial on healthy ageing achieves first milestone
7. Drug resistance-associated genes: A cornerstone for the control and protection against tuberculosis
8. Declining fortunes of Yellowstones migratory elk
9. Protected areas provide African birds with stepping stones to survival
10. Microbial diversity course designated as a Milestones in Microbiology site
11. Natural keystone molecules punch over their weight in ecosystems
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Cantilever sensory array: The Rosetta Stone for antibiotic resistance?
(Date:4/19/2016)... UAE, April 20, 2016 The ... as a compact web-based "all-in-one" system solution for all ... fingerprint reader or the door interface with integration authorization ... access control systems. The minimal dimensions of the access ... into the building installations offer considerable freedom of design ...
(Date:4/14/2016)... April 14, 2016 BioCatch ... Detection, today announced the appointment of Eyal Goldwerger ... role. Goldwerger,s leadership appointment comes at a ... of the deployment of its platform at several of ... technology, which discerns unique cognitive and physiological factors, is ...
(Date:3/31/2016)... R.I. , March 31, 2016  Genomics firm ... of founding CEO, Barrett Bready , M.D., who ... members of the original technical leadership team, including Chief ... President of Product Development, Steve Nurnberg and Vice President ... returned to the company. Dr. Bready served ...
Breaking Biology News(10 mins):
(Date:6/27/2016)... ... June 27, 2016 , ... Rolf ... join the faculty of the University of North Carolina Kenan-Flagler Business School ... and entrepreneurship at UNC Kenan-Flagler, with a focus on the school’s international efforts, ...
(Date:6/24/2016)... ... 2016 , ... While the majority of commercial spectrophotometers and fluorometers use the ... models are higher end machines that use the more unconventional z-dimension of 20mm. ... the bottom of the cuvette holder. , FireflySci has developed several Agilent flow ...
(Date:6/23/2016)... 2016 /PRNewswire/ - FACIT has announced the creation ... biotechnology company, Propellon Therapeutics Inc. ("Propellon" or "the ... a portfolio of first-in-class WDR5 inhibitors for the ... WDR5 represent an exciting class of therapies, possessing ... for cancer patients. Substantial advances have been achieved ...
(Date:6/23/2016)... , June, 23, 2016  The Biodesign Challenge ... envision new ways to harness living systems and biotechnology, ... Art (MoMA) in New York City ... 130 participating students, showcased projects at MoMA,s Celeste Bartos ... Paola Antonelli , MoMA,s senior curator of architecture and ...
Breaking Biology Technology: