Navigation Links
Cancer gene therapy from camels
Date:7/14/2011

Nanobodies produced from camel blood have unique properties, which can be used in future drug development. New research published in Journal of Controlled Release confirms that camel blood can help scientists in the fight against cancer.

Members of the camelid family have particular heavy-chain antibodies. These antibodies can be used to clone nanobodies, which are antibody-derived therapeutic proteins. One of the most powerful advantages of nanobodies is that they can be easily attached to other proteins and nanoparticles by simple chemical procedures.

Scientists at the Department of Pharmaceutics and Analytical Chemistry, University of Copenhagen, have designed nanoparticle systems of smaller than 150nm that are decorated with nanobodies expressing high specificity for the cancer marker Mucin-1, which is connected to breast and colon cancer.

Research supports aim for safer nanomedicines

"This is a very effective and a highly promising approach in experimental cancer gene therapy, while minimising adverse-related reactions to cancer nanomedicines. Futhermore the research supports our aim for rational design and engineering of effective and safer nanomedicines for the future. We have taken the first step, but of course more work is needed to support the efficacy of this system for cancer treatment," says Professor Moein Moghimi.

Professor Moghimi works at the Department of Pharmaceutics and Analytical Chemistry at the Faculty of Pharmaceutical Sciences where he heads the Centre for Pharmaceutical Nanotechnology and Nanotoxicology, which is supported by the Danish Ministry of Science, Technology and Innovation.

The procedures for camel immunisation, generation and purification of the Mucin-1 nanobody were done by Dr. Fatemeh Rahbarizadeh's team at the Medical Biotechnology Department of the Tarbiat Modares University in Tehran. Dr. Rahbarizadeh is currently visiting scientist at the University of Copenhagen.

Two postdocs, Davoud Ahmadvand and Ladan Parhamifar, from the Faculty of Pharmaceutical Sciences, University of Copenhagen, were also involved in the lab work.

Size and properties matter

Compared to other protein-based drugs, nanobodies are very small. They are ten times smaller than intact antibodies. They are also less sensitive to temperature and pH changes and can be easily linked to nanoparticles and other proteins. These properties make nanobodies very interesting for targeting of cancer cells.

The recently published article in Journal of Controlled Relase describes how a Mucin-1 nanobody was linked to specialised nanoparticles made from polymers carrying a killer gene known at truncated-Bid. When expressed, the gene product triggers cells to commit suicide.

However, the expression of the killer gene was under the control of the cancer-specific Mucin-1 promoter as to avoid non-specific cell killing. These procedures are also referred to as "transcriptional targeting", which can prevent normal tissue toxicities associated with other cancer treatments. Indeed, the formulation proved to be highly effective in killing cancer cells expressing the Mucin-1 marker, while no harm was done to the normal cells or cancer cells that did not express the Mucin-1 marker.

The efficacy of these nanoparticles is now being tested in animal models.

Another exciting development is that the team has now purified a second and a highly effective nanobody against another cancer marker (Her-2) expressed by certain breast tumors.


'/>"/>

Contact: Moein Moghimi
momo@farma.ku.dk
455-121-7975
University of Copenhagen
Source:Eurekalert

Related biology news :

1. Cancer data not readily available for future research
2. UT faculty improving surgical outcomes for children, cancer patients
3. Study explains why men are at higher risk for stomach cancer
4. Stem cells restore cognitive abilities impaired by brain cancer treatment
5. PSA test for men could get a second life for breast cancer in women
6. Low dose naltrexone (LDN): Harnessing the bodys own chemistry to treat human ovarian cancer
7. GNS Healthcare Collaborates with NCI in New Approach to Lung Cancer; Supercomputer Analysis to Aid in Matching Targeted Drugs to Patients
8. AACR hosts Frontiers in Basic Cancer Research Conference in San Francisco
9. University of Houston researcher an author of multi-institutional genetic study of ovarian cancer
10. TGen presents lung cancer studies at Amsterdam conference
11. Southern US states lag in reducing death rates from colorectal cancer
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/15/2016)... April 15, 2016  A new partnership announced ... accurate underwriting decisions in a fraction of the ... priced and high-value life insurance policies to consumers ... With Force Diagnostics, rapid testing (A1C, Cotinine ... readings (blood pressure, weight, pulse, BMI, and activity ...
(Date:3/31/2016)... RATON, Florida , March 31, 2016 ... LEGX ) ("LegacyXChange" or the "Company") ... for potential users of its soon to be launched ... video ( https://www.youtube.com/channel/UCyTLBzmZogV1y2D6bDkBX5g ) will also provide ... the use of DNA technology to an industry that ...
(Date:3/22/2016)... 2016 According to ... for Consumer Industry by Type (Image, Motion, Pressure, ... & IT, Entertainment, Home Appliances, & Wearable ... 2022", published by MarketsandMarkets, the market for ... USD 26.76 Billion by 2022, at a ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... June 23, 2016 , ... Mosio, ... second eBook, “Clinical Trials Patient Recruitment and Retention Tips.” Partnering with experienced clinical ... eBook by providing practical tips, tools, and strategies for clinical researchers. , “The ...
(Date:6/23/2016)... June 23, 2016 Houston Methodist Willowbrook ... Cy-Fair Sports Association to serve as their official ... Houston Methodist Willowbrook will provide sponsorship support, athletic ... with association coaches, volunteers, athletes and families. ... Cy-Fair Sports Association and to bring Houston Methodist ...
(Date:6/23/2016)... ... , ... Supplyframe, the Industry Network for electronics hardware design ... Located in Pasadena, Calif., the Design Lab’s mission is to bring together inventors ... and brought to market. , The Design Lab is Supplyframe’s physical representation of ...
(Date:6/23/2016)... 2016 Andrew D ... http://doi.org/10.17925/OHR.2016.12.01.22 Published recently in ... from touchONCOLOGY, Andrew D Zelenetz , discusses ... care is placing an increasing burden on healthcare ... therapies. With the patents on many biologics expiring, ...
Breaking Biology Technology: