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Caltech engineers build smart petri dish
Date:10/3/2011

s a scanning light source. The device is placed in an incubator with a wire running from the chip to a laptop outside the incubator. As the image sensor takes pictures of the culture, that information is sent out to the laptop, enabling the researchers to acquire and save images of the cells as they are growing in real time. The technology is particularly adept at imaging confluent cells -- those that grow very close to one another and typically cover the entire petri dish.

"Until now, imaging of confluent cell cultures has been a highly labor-intensive process in which the traditional microscope has to serve as an expensive and suboptimal workhorse," says Changhuei Yang, senior author of the study and professor of electrical engineering and bioengineering at Caltech. "What this technology allows us to do is create a system in which you can do wide field-of-view microscopy imaging of confluent cell samples. It capitalizes on the use of readily available image-sensor technology, which is found in all cell-phone cameras."

In addition to simplifying medical diagnostic tests, the ePetri platform may be useful in various other areas, such as drug screening and the detection of toxic compounds. It has also proved to be practical for use in basic research.

Caltech biologist Michael Elowitz, a coauthor on the study, has put the ePetri system to the test, using it to observe embryonic stem cells. Stem cells in different parts of a petri dish often behave differently, changing into various types of other, more specialized cells. Using a conventional microscope with its lens's limitations, a researcher effectively wears blinders and is only able to focus on one region of the petri dish at a time, says Elowitz. But by using the ePetri platform, Elowitz was able to follow the stem-cell changes over the entire surface of the device.

"It radically reconceives the whole idea of what a light microscope is," says Elowitz, a professor of biology a
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Contact: Deborah Williams-Hedges
debwms@caltech.edu
626-395-3227
California Institute of Technology
Source:Eurekalert  

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