Navigation Links
Caltech and UCSD researchers shed light on how proteins find their shapes
Date:2/23/2009

PASADENA, Calif.--Researchers from the California Institute of Technology (Caltech) and the University of California at San Diego (UCSD) have brought together UCSD theoretical modeling and Caltech experimental data to show just how amino-acid chains might fold up into unique, three-dimensional functional proteins.

Their insights were recently published in the February 10 issue of the Proceedings of the National Academy of Sciences (PNAS).

The paper details the matching of a series of protein-folding models created by the UCSD team (led by Peter Wolynes, UCSD professor of chemistry and biochemistry and physics) with experimental data gathered using a novel technique created by the Caltech team (led by Faculty Associate in Chemistry Jay Winkler and Harry Gray, Caltech's Arnold O. Beckman Professor of Chemistry and founding director of the Beckman Institute).

The Winkler-Gray method of watching proteins as they crumple and fold involves the use of a picosecond camera that captures fluorescent flashes as a laser pulse excites a donor probe, which emits light and transfers that light to an acceptor probe. The distance between the donor and acceptor change as the amino-acid chain transforms itself into a three-dimensional protein.

In the PNAS paper, the two groups combined the Caltech experimental technique--first described in a 2002 paper published in the Journal of the American Chemical Society--with Wolynes's protein-folding models to see if they could come up with the precise folding pattern of cytochrome c, a protein that is part of the mitochondrial electron-transfer chain that turns food into cellular energy.

At first the models and the experimental data seemed to be describing two entirely different things, according to Winkler. "The researchers had to account for charge-charge interactions between amino acids that appear to be important--the way that like charges repel and opposite charges attract," he explains. "And they had to consider the hydrophobic interactions--the way that oily parts of the proteins like to stick together but are repelled by the watery parts. When their models took account of these interactions, it fit the experimental data."

"It was the first time anyone has been able to develop a theoretical model able to account for the results we've been getting with our time-resolved energy-transfer experiments," adds Gray.


'/>"/>

Contact: Lori Oliwenstein
lorio@caltech.edu
626-395-3631
California Institute of Technology
Source:Eurekalert

Related biology news :

1. UNC, Caltech research finds further evidence for genetic contribution to autism
2. Caltech researchers find dual-use sexual attraction and population-control chemicals in nematodes
3. Caltech scientists awarded $20 million to Power the Planet
4. Caltech neurobiologists discover individuals who hear movement
5. Caltech engineers build mini drug-producing biofactories in yeast
6. Caltech researchers awarded $10M for molecular programming project
7. Caltech scientists create DNA tubes with programmable sizes for nanoscale manufacturing
8. Caltech biologists spy on the secret inner life of a cell
9. Caltech scientists engineer supersensitive receptor, gain better understanding of dopamine system
10. Caltech engineers build firast-ever multi-input plug-and-play synthetic RNA device
11. Caltech geobiologists discover unique magnetic death star fossil
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/14/2016)... BioCatch ™, the global ... the appointment of Eyal Goldwerger as CEO. ... Goldwerger,s leadership appointment comes at a time of significant ... of its platform at several of the world,s largest ... unique cognitive and physiological factors, is a winner of ...
(Date:3/31/2016)... March 31, 2016   ... or the "Company") LegacyXChange is excited to ... its soon to be launched online site for trading ... ) will also provide potential shareholders a sense ... technology to an industry that is notorious for fraud. ...
(Date:3/29/2016)... , March 29, 2016 ... "Company") LegacyXChange "LEGX" and SelectaDNA/CSI Protect are pleased to ... ink used in a variety of writing instruments, ensuring ... of originally created collectibles from athletes on LegacyXChange will ... analysis of the DNA. Bill Bollander ...
Breaking Biology News(10 mins):
(Date:5/20/2016)... (PRWEB) , ... May 20, 2016 , ... Kablooe Design, ... goods companies, today announced its official 25th anniversary of the business. “We have worked ... so grateful to our customers for the privilege and honor of serving their product ...
(Date:5/19/2016)... ... May 19, 2016 , ... Anton Paar USA, located ... foot office building is complete. The new structure adds a third office building to ... Paar USA purchased 2.4 acres of land, along with office space adjacent to ...
(Date:5/19/2016)... ... May 19, 2016 , ... KCAS Bioanalytical and ... Siddiqui as Director, Large Molecule & Biomarker Bioanalysis. , Dr. Siddiqui has more ... discovery studies for preclinical and clinical safety programs. “We’ve seen significant demand for, ...
(Date:5/18/2016)... ... 18, 2016 , ... Tech Coast Angels (TCA) today announced that ... at the annual ACA Summit last week in Philadelphia. , The award ... member angel groups. It is the highest honor available for an early-stage company to ...
Breaking Biology Technology: