Navigation Links
CSI: Microbial version without PCR
Date:9/21/2011

s," Andersen says. "The choice of which of these two PCR-free methods to use is case-by-case. Some samples work fine with the simple and direct rRNA method while others require the additional steps of the double-stranded cDNA method."

Andersen, DeAngelis and their colleagues successfully tested their two new PCR-free analysis techniques by comparing results with PCR-amplified DNA tests using a a mock community of eight microbial taxa in three environmental sample types: chromium-contaminated aquifer groundwater; tropical forest soil; and secondary sewage in sea water.

"Community profiles produced by our alternative methods showed taxa differences that would be expected based on accompanying data, but were missing in PCR-amplified communities," DeAngelis says. "Our result show that a PCR-independent view of microbial communities can potentially change which microbes we think are dominant in an ecosystem. At JBEI, for example, we can use these new methods to identify microbes that are most active in breaking down feedstock biomass, such as switchgrass, into sugars that can be synthesized into advance biofuels."


'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Tuning natural antimicrobials to improve their effectiveness at battling superbugs
2. Handbook of Molecular Microbial Ecology: Exploring the World of the Microbes
3. 51st Interscience Conference on Antimicrobial Agents and Chemotherapy
4. E. coli bacteria more likely to develop resistance after exposure to low levels of antibiotics, reports a study in Microbial Drug Resistance
5. Tut, tut: Microbial growth in pharaohs tomb suggests burial was a rush job
6. Stevens conference explores latest innovations in antimicrobial biomaterials research
7. New study pinpoints why some microbial genes are more promiscuous than others
8. Study finds anti-microbials a common cause of drug-induced liver injury and failure
9. Busy microbial world discovered in deepest ocean crust ever explored
10. Reading the signs: Plants and animals found common ground in response to microbial threats
11. Myth of a germ-free world: A closer look at antimicrobial products
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
CSI: Microbial version without PCR
(Date:6/23/2015)... GARDENS, Fla. , June 23, 2015 ... identity management and authentication solutions, today announced enhanced ... strong, multi-factor authentication solution.  The enhancements build ... use of the DigitalPersona Altus platform and ... reader compatibility. In today,s environment ...
(Date:6/17/2015)... GERMANTOWN, Maryland , June 17, 2015 ...   Produktlinie mit DNA-Tests verbessert den Analyseprozess und ... QGEN ; Frankfurt Prime Standard: QIA) hat heute in ... Produktlinie Investigator ® für ... Die neuen Kits zur Erstellung genetischer Fingerabdrücke bieten eine ...
(Date:6/17/2015)... , June 17, 2015  Crossmatch™, a leading ... that its U.are.U ® 4500 fingerprint readers ... Eateries, Inc., a San Jose, ... to increase security, improve accountability and reduce fraud ... instant, non-repudiable identity confirmation for employee sign in ...
Breaking Biology News(10 mins):Crossmatch Releases Enhanced DigitalPersona Altus Advanced Authentication Functionality 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 2QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 3QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 4QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 5QIAGEN bringt in den USA neue Kits für die forensische DNA-Analyse auf den Markt 6Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 2Togo's Eateries Improve Security and Accountability with Crossmatch Fingerprint Readers 3
... ARBOR, Mich. An early laboratory success is taking ... gland transplants that could one day prevent a currently ... The scientists were able to induce embryonic stem ... hormone essential to maintaining bone density. The laboratory results ...
... ANN ARBOR, Mich.---Brain implants that can more clearly record ... at the University of Michigan. The findings could eventually ... as Parkinson,s disease and paralysis. Neural electrodes must ... When the electrodes are implanted, the brain first reacts ...
... calling on Congress to support the Consistency, Accuracy, Responsibility ... (CARE bill), which would ensure that states set minimum ... bill (H.R. 3652) was introduced Sept. 28 in the ... "The introduction of the CARE bill ...
Cached Biology News:Stem cell success points to way to regenerate parathyroid glands 2A step toward better brain implants using conducting polymer nanotubes 2A step toward better brain implants using conducting polymer nanotubes 3SNM urges Congress to support CARE bill 2
(Date:6/30/2015)... WA (PRWEB) , ... June 30, 2015 , ... ... a hydrophilic filter that does not sacrifice selectivity to achieve faster flow rates ... combines extremely fine pores with significant open space to allow easy liquid flow ...
(Date:6/30/2015)... ... June 30, 2015 , ... Matrix Marketing Group, LLC , ... hired Gem Swartz to head up its account management department as vice president of ... and Amanda bring senior account management and delivery expertise to the growing Matrix Marketing ...
(Date:6/30/2015)... 2015 /PRNewswire/ - RepliCel Life Sciences Inc. (OTCQB: ... company focused on the development of autologous cell ... Phase 1/2 clinical trial of RCT-01, being tested ... has been enrolled and their tissue biopsy sent ... is comprised of non-bulbar dermal sheath (NBDS) cells ...
(Date:6/30/2015)... ... June 30, 2015 , ... ... SmartArch — a revolutionary new archwire that allows orthodontists to simultaneously apply different ... of the efficacy of SmartArch. “We’re thrilled about taking SmartArch one step closer ...
Breaking Biology Technology:New Nanofiber Membrane Provides Fast, Efficient Filtration 2Matrix Marketing Group Expands Client Services Team 2RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 2RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 3RepliCel Life Sciences Enrolls First Participant in Phase 1/2 Clinical Trial of RCT-01 for Chronic Achilles Tendinosis 4New case studies for SmartArch orthodontic archwire paves way to product launch later this year 2
... Multiporator / Electroporator 2510 , , , , , , , ... Protocol No. 4308 915.521 02/2002 , , , ... , Microorganism , ... Cell type , Bacteria, gram positive, , ...
... , , , , Multiporator / Electroporator 2510 , , , ... Transformation Protocol , Protocol No. 4308 915.514 04/2002 , ... , , , , ... , Cell type , Bacteria, ...
... , , , , Multiporator / Electroporator 2510 , , , ... Transformation Protocol , Protocol No. 4308 915.511 04/2002 , ... , , , , ... , Cell type , Bacteria, gram ...
Cached Biology Technology:Mycobacterium intracellulare 2Escherichia coli K12 2Escherichia coli K12 3Escherichia coli C600 2Escherichia coli C600 3
Mouse monoclonal antibody raised against a partial recombinant STAU2. NCBI Entrez Gene ID = STAU2...
...
Mouse polyclonal antibody to NR2F2 - nuclear receptor subfamily 2, group F, member 2...
... Pack provides optimized blocking and wash ... Clarifier: Ready-to-use wash solutions have been ... Coated Slides for Microarrays (Product Code ... Buffer: 1L • Microarray Post Hybridization ...
Biology Products: