Navigation Links
CSI: Microbial version without PCR
Date:9/21/2011

Anyone who has watched one of the CSI: Crime Scene Investigation television shows knows that PCR (Polymerase Chain Reaction) is a technology used to amplify the tiniest samples of DNA into forensic evidence that can identify perpetrators or victims of a crime. Microbiologists also use PCR to uncover the identity of microbes in samples taken from a wide range of sources for a wide range of purposes. However, for microbial analysis, the use of PCR technology can pose problems. Now, researchers with the U.S. Department of Energy (DOE)'s Lawrence Berkeley National Laboratory (Berkeley Lab) have overcome those problems with the development of PCR-free technology that is based on Berkeley Lab's award-winning, high-density DNA-based microarray known as the PhyloChip.

"We've developed two methods of PCR-independent microbial community analysis using the PhyloChip," says Gary Andersen, a microbial ecologist with Berkeley Lab's Earth Sciences Division. "Each method represents a simple and economical way to directly query microbial communities in natural environments and each offers the additional advantage of detecting the most metabolically active microbes in a community, the ones most likely to attenuate toxins, drive biogeochemical cycles or proliferate in disease states."

Andersen is the corresponding author of a paper published in the journal Applied and Environmental Microbiology that discusses this research. The paper is titled "PCR amplification-independent methods for detection of microbial communities by the high density microarray PhyloChip." Co-authoring the paper were Kristen DeAngelis, Cindy Wu, Harry Beller, Eoin Brodie, Romy Chakraborty, Todd DeSantis, Julian Fortney, Terry Hazen, Shariff Osman, Mary Singer and Lauren Tom.

How omnipresent are microbes in the environment? Extract a gram of soil from just about anywhere and you can expect to find many millions of individual microbes representing more than a thousand differe
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Tuning natural antimicrobials to improve their effectiveness at battling superbugs
2. Handbook of Molecular Microbial Ecology: Exploring the World of the Microbes
3. 51st Interscience Conference on Antimicrobial Agents and Chemotherapy
4. E. coli bacteria more likely to develop resistance after exposure to low levels of antibiotics, reports a study in Microbial Drug Resistance
5. Tut, tut: Microbial growth in pharaohs tomb suggests burial was a rush job
6. Stevens conference explores latest innovations in antimicrobial biomaterials research
7. New study pinpoints why some microbial genes are more promiscuous than others
8. Study finds anti-microbials a common cause of drug-induced liver injury and failure
9. Busy microbial world discovered in deepest ocean crust ever explored
10. Reading the signs: Plants and animals found common ground in response to microbial threats
11. Myth of a germ-free world: A closer look at antimicrobial products
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
CSI: Microbial version without PCR
(Date:9/18/2014)... 2014 -- Arizona turned its newborn screening program into a ... months, earning it the first-ever Newborn Screening Quality Award from ... presented Will Humble, M.P.H., Arizona,s Department of Health Services director, ... Humble established a policy of full transparency for the length ... samples to the lab for analysis, and set a target ...
(Date:9/18/2014)... which have devastated algal forests in the eastern Mediterranean ... basin if their distribution continues to expand as the ... an international team of researchers led by Dr Adriana ... the Mediterranean Institute for Advanced Studies in Spain, is ... of the team surveyed more than 1000 kilometres of ...
(Date:9/18/2014)... the fish world, are the ideal animal in which ... moved from the ocean into tens of thousands of ... changing their skeleton to adapt to the new environment. ... sticklebacks now have turned up one of the genes ... change in that gene,s regulation in a freshwater population ...
Breaking Biology News(10 mins):March of Dimes honors Arizona with first-ever newborn screening award 2March of Dimes honors Arizona with first-ever newborn screening award 3Tropical fish a threat to Mediterranean Sea ecosystems 2Counting fish teeth reveals regulatory DNA changes behind rapid evolution, adaptation 2Counting fish teeth reveals regulatory DNA changes behind rapid evolution, adaptation 3
... When the larvae of the primitive social insect Polistes ... she doesnt know if shell arise a worker or gyne ... social insect researcher Gro Amdam. Amdams group is ... solitary ancestors through study of a primitive social order of ...
... Vitamin D deficiency early in pregnancy is associated with ... study from the University of Pittsburgh Schools of the ... Clinical Endocrinology and Metabolism. A serious complication of ... the hands and feet, preeclampsia is the leading cause ...
... researchers at Boston Universitys College of Engineering may ... capable of defeating so-called superbugs, bacteria that have ... discovered a previously unknown chain of events occurring ... avenues of research. Currently, three classes ...
Cached Biology News:Old developmental pathways spawn revolutionary evolutionary changes 2Old developmental pathways spawn revolutionary evolutionary changes 3Low vitamin D during pregnancy linked to pre-eclampsia 2Boston University biomedical engineers find chink in bacteria's armor 2Boston University biomedical engineers find chink in bacteria's armor 3
(Date:9/18/2014)... Woburn, MA (PRWEB) September 18, 2014 ... Single Molecule Sequencing (tSMS™) technology, announced today the ... round of investment, led by Genomic Diagnostic Technologies, ... scientific and diagnostic applications. , Founded in March ... Scientist and Bioinformatics Analyst at Helicos BioSciences, SeqLL ...
(Date:9/18/2014)... to weight loss and diabetes prevention, could actually hasten ... they do it in a surprising way: by changing ... the substantial population of bacteria residing in our intestines. ... humans, were published today in Nature . Among ... Institute,s Immunology Department, who led this research together with ...
(Date:9/18/2014)... quest for the ultimate memory device for computing may ... The City College of New York led by chemist ... both magnetic and ferroelectric properties. , Combining both properties ... occur between them and for the devices that might ... these two properties in a single material, however, has ...
(Date:9/18/2014)... MX (PRWEB) September 18, 2014 September ... Mexico City, MX. ASEA is the manufacturer of the ... the skin care product, RENU28. , The Mexico ... Vice President Duvan Botero, and ASEA Field Leader Shawn ... holds a PhD in Atomic/Medical Physics from the University ...
Breaking Biology Technology:SeqLL Closes Series-A Funding Round for Expansion of Single Molecule Sequencing 2Gut bacteria, artificial sweeteners and glucose intolerance 2Gut bacteria, artificial sweeteners and glucose intolerance 3Oxides discovered by CCNY team could advance memory devices 2Announcing the ASEA Mexico Grand Opening Tour Beginning September 18th 2
... Keck Graduate Institute,(KGI) today announced a partnership with ... forum to help women managers advance in the,life ... forum from Jan. 13-18, 2008. Attendees will,have the ... executives from,pharmaceutical, medical device and biotechnology companies along ...
... 21 Nile Therapeutics Inc., (OTC,Bulletin Board: SPDU) ... disease, is pleased to announce the,appointments of Daron ... as the Vice President of Development. Mr. ... Chief Operating Officer,and was appointed Chief Financial Officer ...
... of Physics Dr. Satyendra Kumar recently was honored ... Directors Award for Excellence for recognition of exceptional ... matter physics proposals. In all, seven program directors ... Arlington, Va. , The NSF Directors Award ...
Cached Biology Technology:KGI and Smith College Establish Leadership Forum for Women in Bioscience 2Nile Therapeutics, Inc. Adds to Executive Management Team 2Nile Therapeutics, Inc. Adds to Executive Management Team 3Nile Therapeutics, Inc. Adds to Executive Management Team 4
... Detection Systems allow non-radioactive detection of ... systems, biotinylated lysine residues are incorporated ... the need for labeling with [35-S]methionine ... biotinylated lysine is added to the ...
... Series Hybridization chambers with forced convection ... ambient up to 99.9 C / 212 F ... and reproducible results DS controller Timer ... with an accuracy of a tenth of a ...
... of your microarray data using Asuragens bioinformatics ... from consultation to extensive data analysis using ... our expertise in combination with the Gene ... microarray experiments., ,Normalization and basic statistical analysis ...
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. Material: cap ...
Biology Products: