Navigation Links
CRISPR critters: Scientists identify key enzyme in microbial immune system
Date:9/9/2010

the RNA recognition mechanisms in Cys4 and Dicer, the enzyme that plays a critical role in eukaryotic RNA interference."

Doudna is a leading authority on RNA molecular structures who holds joint appointments with Berkeley Lab's Physical Biosciences Division and UC Berkeley's Department of Molecular and Cell Biology and Department of Chemistry. She is also an investigator with the Howard Hughes Medical Institute (HHMI). The results of this latest research on CRISPR are reported in the journal Science in a paper titled "Sequence- and structure-specific RNA processing by a CRISPR endonuclease." Co-authoring the paper with Doudna were Rachel Haurwitz, Martin Jinek, Blake Wiedenheft and Kaihong Zhou.

CRISPR is a unit of DNA, usually on a microbe's chromosome, made up of "repeat" elements, base-pair sequences ranging from 30 to 60 nucleotides in length, separated by "spacer" elements, variable sequences that are also from 30 to 60 nucleotides in length. CRISPR units are found in about 40-percent of all bacteria whose genomes have been sequenced, and about 90-percent of archaea. A microbe might have several CRISPR loci within its genome and each locus might contain between four and 100 CRISPR repeat-spacer units. Doudna and her colleagues studied CRISPR in Pseudomonas aeruginosa, a common bacterium that is ubiquitous in the environment.

Rachel Haurwitz, a graduate student in Doudna's research group and the first author on the Science paper, explains how the CRISPR/Cas immunity system works.

"When a bacterium recognizes that it has been invaded by a virus or a plasmid, it incorporates a small piece of the foreign DNA into one of its CRISPR units as a new spacer sequence. The CRISPR unit is then transcribed as a long RNA segment called the pre-crRNA. The Csy4 enzyme cleaves this pre-crRNA within each repeat element to create crRNAs about 60 nucleotides long that will contain sequences which match portions of the foreign DN
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Jefferson scientists deliver toxic genes to effectively kill pancreatic cancer cells
2. Scientists identify novel inhibitor of human microRNA
3. Argonne scientists peer into heart of compound that may detect chemical, biological weapons
4. MU scientists go green with gold, distribute environmentally friendly nanoparticles
5. Scientists identify gene that may contribute to improved rice yield
6. Scientists discover why a mothers high-fat diet contributes to obesity in her children
7. MU scientists see how HIV matures into an infection
8. Earth scientists keep an eye on Texas
9. Thinking it through: Scientists call for policy to guide biofuels industry toward sustainability
10. Scientists identify a molecule that coordinates the movement of cells
11. Scientists Find new migratory patterns for Mediterranean and Western Atlantic bluefin tuna
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
CRISPR critters: Scientists identify key enzyme in microbial immune system
(Date:8/27/2014)... South Africa is entering what is described by the ... Season." The Eastern Cape provincial government warned residents ... of strong winds and veld fires. A high ... interior and along the coast in the Great Kei ... coastal regions, and during thunderstorms. The thunderstorms bring ...
(Date:8/27/2014)... number of consumers are willing to pay a premium ... whether they,re getting what the label claims is another ... devising a new way to make sure farms are ... ACS, Journal of Agricultural and Food Chemistry , ... the Bavarian Health and Food Safety Authority and the ...
(Date:8/27/2014)... have shown that excessive iron is closely ... Previous studies from Chunyan Guo and co-workers ... shown that baicalin prevented iron accumulation after ... 1 expression, and increased ferroportin 1 expression ... disease rats. However, the relationship between iron ...
Breaking Biology News(10 mins):
... oral drug therapy called fenobam has shown promising initial results ... patients with Fragile X syndrome (FXS). Findings of the ... Center and the University of California, Davis, Medical Center are ... Journal of Medical Genetics . Results ...
... Human Genome Center at Shanghai (South Center, hereafter) was ... in Shanghai. To commemorate this important event, ,which marks ... specially organize a series of mini-reviews for ,this special ... attention of the Chinese life science research workers ,to ...
... MADISON, WI, JANUARY 5, 2009 Currently, plant ... ecological risks from chemical pesticides to nontarget plants ... plants. Protection of the plant development and reproduction ... protection of animals. Past research conducted by the ...
Cached Biology News:Promising new drug being evaluated as possible treatment option for fragile X syndrome 2Human genomics in China 2Human genomics in China 3Human genomics in China 4Human genomics in China 5Human genomics in China 6Human genomics in China 7Human genomics in China 8Human genomics in China 9Human genomics in China 10Human genomics in China 11
(Date:8/27/2014)... Rhythm, a biopharmaceutical company developing peptide therapeutics that address ... disorders, announced today that it has filed a registration ... Exchange Commission (SEC) relating to the proposed initial public ... of shares to be offered and the price range ... Citigroup and Cowen and Company will act as ...
(Date:8/27/2014)... The Global and China Trifluoroacetic Acid Industry ... state of the Trifluoroacetic Acid industry in Global and ... the industry, including definitions, applications and industry chain structure. ... provided with a focus on history, developments, trends and ... international and Chinese situation is also offered. , The ...
(Date:8/27/2014)... (PRWEB) August 27, 2014 “We are ... technology vendors,” said Ven Thangaraj, CEO of Radiant Sage. ... that are taking the imaging corelab market to a ... corelabs and research organizations to efficiently and effectively manage ... the data to be analyzed and read by trained ...
(Date:8/27/2014)... DUBLIN , Aug. 27, 2014 ... "Global Stable Isotope Ratio Mass Spectrometer Industry Report ... The Global Stable Isotope Ratio Mass Spectrometer Industry Report ... current state of the global isotope ratio mass spectrometer ... of the industry including definitions, classifications, applications and industry ...
Breaking Biology Technology:Rhythm Files Registration Statement for Proposed Initial Public Offering 2Trifluoroacetic Acid Industry (Asia, Europe, North America, ROW) Analysis Report Now Available at ReportsnReports.com 2Radiant Sage Named to CIOReview List of 100 Most Promising Technology Companies 2Global Stable Isotope Ratio Mass Spectrometer Industry Report 2014 2
... May 21, 2012  The Large Urology Group ... urologists, today discredited recommendations by the U.S. Preventive ... testing. USPSTF,s final recommendation, released today, ... the PSA test. This comes despite overwhelming opposition ...
... Biopharma, Inc. (OTC/BB: GNBP), a biotechnology company developing targeted ... to Shareholders to the Investors section of the Company,s ... 15, 2012, the Company filed with the U.S. Securities ... months ended March 31, 2012 on Form 10-Q.  The ...
... NEW YORK, May 18, 2012  Intellect Neurosciences, Inc. ... the company,s chairman and CEO, will be presenting ... Conference. (Logo:  http://photos.prnewswire.com/prnh/20111214/NY22484LOGO ) ... is "Prefibrillar Tau Neoepitopes Offer Attractive Targets for ...
Cached Biology Technology:Urologists Outraged over Government Panel's Recommendation to Stop Life-Saving Prostate Cancer Testing 2Urologists Outraged over Government Panel's Recommendation to Stop Life-Saving Prostate Cancer Testing 3Genesis Biopharma Issues Letter To Shareholders 2Genesis Biopharma Issues Letter To Shareholders 3Genesis Biopharma Issues Letter To Shareholders 4Intellect Neurosciences, Inc. to Present at Neurotech Investing and Partnering Conference 2Intellect Neurosciences, Inc. to Present at Neurotech Investing and Partnering Conference 3
This free-standing laminar flow hood provides a vertical flow of HEPA-filtered air to create a clean processing area. It includes a fluorescent light and motorized sliding shield. Options include wor...
... This CLS number is a ... easily match Cornings product number. ... please order under the old ... customer service for assistance. ID ...
... GyroTwister GX-1000 3-D Shaker , ... shaking motion of the GyroTwister GX-1000 is ... ideal for general mixing as well as ... speed is continuously adjustable across a broad ...
...
Biology Products: