The Genomic Instability Group led by researcher scar Fernndez-Capetillo at the Spanish National Cancer Research Centre (CNIO), has for the first time obtained a panoramic photo of the proteins that take part in human DNA division, a process known as replication.
The research article, published today in the journal Cell Reports, is the result of a collaborative study in which other CNIO groups have also participated, including the Proteomics Unit led by Javier Muoz and the DNA Replication Group led by Juan Mndez.
DNA replication is the chemical process that sustains cell division, and thus one of the biological mechanisms targeted by most chemotherapeutic agents in order to destroy tumour cells.
To date, multiple independent molecular studies carried out over the last decades have given a general idea of the proteins involved in the replication process. "We suspected that there might be several dozen proteins that control this process meticulously, thus ensuring the correct duplication of our genome as an indispensible step prior to cell division," explains Fernndez-Capetillo.
Thanks to the development of a new technology that allows to isolate recently synthesised DNA, in addition to sophisticated proteomic detection tools (the iPOND-MS technique), CNIO researchers have for the first time been able to precisely draw out, in a single experiment, the replication machinery. These results represent the first proteomic characterisation of the replisome.
According to the authors, the proteins identified have very different activities: they open up the DNA double helix, copy it, repair any breaks if needs be, modify it in different ways, etc. "In short, they're all necessary in order to ensure the correct duplication of the DNA and avoid aberrations in the genetic material that form the basis of tumours", states Fernndez-Capetillo.
NEW REPLICATION PROTEINS
Andrs Joaqun Lpez-Contreras, the f
|Contact: Nuria Noriega|
Centro Nacional de Investigaciones Oncologicas (CNIO)