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Building artificial cells will be a noisy business
Date:2/24/2014

ny tiny oscillators. Using an approach developed by Maximilian Weitz and colleagues at the Technical University of Munich and former Caltech graduate student Elisa Franco, currently an assistant professor of mechanical engineering at UC Riverside, an aqueous solution of the DNA, RNA, and enzymes that make up the biochemical oscillator was mixed with oil and shaken until small portions of the solution, each containing a tiny oscillator, were isolated within droplets surrounded by oil.

"After the oil is added and shaken, the mixture turns into a cream, called an emulsion, that looks somewhat like a light mayonnaise," says Winfree. "We then take this cream, pour it on a glass slide and spread it out, and observe the patterns of pulsing fluorescence in each droplet under a microscope."

When a large sample of the solution is active, it fluoresces in regular pulses. The largest droplets behave as the entire solution does: fluorescing mostly in phase with one another, as though separate but still acting in concert. But the behavior of the smaller droplets was found to be much less consistent, and their pulses of fluorescence quickly moved out of phase with the larger droplets.

Researchers had expected that the various droplets, especially the smaller ones, would behave differently from one another due to an effect known as stochastic reaction dynamics. The specific reactions that make up a biochemical circuit may happen at slightly different times in different parts of a solution. If the solution sample is large enough, this effect is averaged out, but if the sample is very small, these slight differences in the timing of reactions will be amplified. The sensitivity to droplet size can be even more significant depending on the nature of the reactions. As Winfree explains, "If you have two competing reactionssay x could get converted to y, or x could get converted to z, each at the same ratethen if you have a test tubesized sample, you will end u
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Contact: Deborah Williams-Hedges
mr@caltech.edu
California Institute of Technology
Source:Eurekalert

Page: 1 2 3

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