Brucella abortus S19 genome sequenced; points toward virul...(nscription and protein transport. We bel...)

The initial sequencing was performed on the Roche GS-20 at 454 Life Sciences, and subsequently repeated after the installation of the Roche GS-FLX in the VBI Core Laboratory Facility. Additional traditional sequencing methods were used in VBI's Core Laboratory Facility to completely finish the S19 genome sequence. Clive Evans, Associate Director of the Core Laboratory Facility at VBI, remarked: "The S19 sequence was the first bacterial genome sequenced at VBI with the new Roche GS-FLX, which was installed in January 2007. The Roche GS-FLX sequence allowed us to verify and improve the original sequence, and reduced the number of gaps that needed to be covered with traditional sequencing methods. We were very pleased with the performance of the Roche GS-FLX."

Bruno Sobral, Executive and Scientific Director of VBI and co-author, remarked: "The complete genome sequence of strain S19 helps us understand the causal molecular agents of brucellosis." He remarked: "Brucella is able to pass from animals to humans with relative ease and poses a significant public health burden for workers in the livestock industry. It is also a possible agent for agricultural, civilian and military bioterrorism. This emphasizes the need for researchers to have a handle on the genetic makeup of the different Brucella strains."

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