Navigation Links
Bringing out the best in X-ray crystallography data
Date:11/5/2013

"Function follows form" might have been written to describe proteins, as the M. C. Escher-esque folds and twists of nature's workhorse biomolecules enables each to carry out its specific responsibilities. Technology's workhorse for determining protein structures is X-ray protein crystallography, in which a beam of x-rays sent through a crystallized protein is scattered by the protein's atoms, creating a diffraction pattern of dots that can be reconstructed by computer into a 3D model.

While synchrotron radiation facilities, such as Berkeley Lab's Advanced Light Source, have been a boon to the field of protein crystallography, providing increasingly higher resolution structures over increasingly shorter time-spans, the technology is still a challenge. For some molecules, especially large molecular complexes, it is often only possible to obtain low-resolution experimental data, which means models are difficult to make and must be manually refined using computer modeling.

"Refinement of protein and other biomolecular structural models against low-resolution crystallographic data has been limited by the ability of current methods to converge on a structure with realistic geometry," says Paul Adams, a bioengineer with Berkeley Lab's Physical Biosciences Division and leading authority on x-ray crystallography, who, starting in 2000, has been leading the development of a highly successful software program called PHENIX (Python-based Hierarchical ENvironment for Integrated Xtallography) that automates crystallography data analysis.

Now, Adams and a team that included Nathaniel Echols in his research group, and Frank DiMaio with the research group of David Baker at the University of Washington, have developed a new method for refining crystallographic data that combines aspects of PHENIX with aspects of Rosetta, the most widely used software for the prediction and design of the three-dimensional structure of proteins and other large biomolecul
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Bringing down the cost of fuel cells
2. Bringing natural history collections out of the dark
3. Roots and microbes: Bringing a complex underground ecology into the lab
4. Bringing measuring accuracy to radical treatment
5. Bringing big data to biodiversity
6. Neuroscience symposium bringing top scientists to Louisville, Aug. 1-2
7. Pitt treats gum disease by bringing needed immune cells to inflamed tissue
8. Dental X-rays linked to common brain tumor
9. Stanford-SLAC team uses X-ray imaging to observe running batteries in action
10. Speed and power of X-ray laser helps unlock molecular mysteries
11. Low-cost carbon capture gets X-rayed
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Bringing out the best in X-ray crystallography data
(Date:11/18/2014)... -- The Parenteral Drug Association (PDA) today confirmed that seven ... at least seven more will participate in the upcoming 2014 ... in Washington D.C. , Dec. 2-4. ... from the regulatory agencies in the United States ... effort to help advance the use of metrics in the ...
(Date:11/11/2014)... -- Forensicon, Inc., a Chicago -based digital ... promotion of Yaniv Schiff from Senior Computer ... new role as Director, he will lead company efforts ... and provide leadership within the company,s digital forensics practice ... Schiff joined Forensicon in 2006 and ...
(Date:11/7/2014)... , November 6, 2014 Leading ... for better mobile security revolutionizing online transactions.  Companies in focus ... Holdings Ltd. (NYSE: BABA ), Google Inc. (NASDAQ: ... ), eBay Inc. (NASDAQ: EBAY ) and MasterCard ... NXTD and NXTDW), a biometric authentication company focused on ...
Breaking Biology News(10 mins):FDA's Janet Woodcock, EMA's Emer Cooke Headline PDA Quality Metrics Conference 2Yaniv Schiff Promoted to Director of Digital Forensics at Forensicon, Inc. 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6
... Tuesday, Nov. 16, 2010 Tying in with ... new pre-conference workshops will debut at this year,s TERMIS-North ... Orlando, Fla. The TERMIS (Tissue Engineering & ... by the Wake Forest Institute for Regenerative Medicine, and ...
... brink of an era of mass extinctions similar to ... disappeared from the Earth, reports a biologist at Penn ... a species-rescue program for Haiti,s threatened frogs and other ... few decades, many Haitian species of plants and animals ...
... SAN DIEGO New research indicates that the ... About two percent of the population has a condition called ... are experienced at once. Although this condition is rare, the ... sometimes overlapping ways to help people interpret and understand their ...
Cached Biology News:Regenerative medicine workshops to debut at TERMIS North America Annual Conference 2Rescue missions underway to save Haiti's species from mass extinctions 2Rescue missions underway to save Haiti's species from mass extinctions 3Rescue missions underway to save Haiti's species from mass extinctions 4Rescue missions underway to save Haiti's species from mass extinctions 5Hearing colors, seeing sounds: New research explores sensory overlap in the brain 2
(Date:11/26/2014)... Whitehouse Laboratories is pleased to ... County (New Jersey) Chamber of Commerce as a ... organization in the North Jersey area, offers Whitehouse ... key clients in the pharmaceutical and biotechnology areas ... Membership enables Whitehouse Laboratories a higher level of ...
(Date:11/26/2014)... 25, 2014 Continuing its award-winning streak, ... three prestigious MarCom Awards for outstanding creative achievement by ... Platinum MarCom Award, the organization’s top honor, in the ... Louis held in April of this year. The event ... attendees. Additionally, Nerium was awarded a Gold MarCom Award ...
(Date:11/26/2014)... Miles Holder, formerly of Micron Research ... Products team as Sales/Marketing Manager. Mr. Holder has over ... for the graphite industry. , “We are thrilled to ... Dave Trinkley, VP of Market and Product Development for ... in the graphite industry, along with his proven track ...
(Date:11/26/2014)... Texas (PRWEB) November 26, 2014 ... Industry is an in-depth research report on the ... the report introduces Palmitic Acid, basic information, including ... policy analysis, and news analysis, etc. , ... analyzes Palmitic Acid market in China ...
Breaking Biology Technology:Whitehouse Laboratories Joins Morris County Chamber of Commerce 2Whitehouse Laboratories Joins Morris County Chamber of Commerce 3Nerium International™ Wins One Platinum, Two Gold MarCom Awards 2Miles Holder Joins Graphel Corporation as Sales/Marketing Manager 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 2Palmitic Acid Industry Global 2014 Analysis & 2020 Forecasts on Market data, Statics, Development Now at ReportsnReports.com 3
... Madison, Wis. Companion bills designed to stimulate ... Legislature and now await the signature of governor Jim ... , ,The bills, Assembly Bill 892 and Senate Bill ... would provide $7.5 million in income tax credits to ...
... - Two weeks after solidifying its global market position with ... its line of working capital as well. The company announced ... three venture capital sources. , ,Imago, a provider of atom ... round of funding with funding led by Draper Fisher ...
... Architecture (SOA), the core of such major product suites as ... business productivity. However, the very openness of SOA can disrupt ... of Sarbanes Oxley Act (SOX), the 2002 federal law that ... , , ,A typical internal control over financial ...
Cached Biology Technology:Broadband deployment measure goes to governor 2Investors commit $3.4 million to Imago 2Managing SOX in the age of SOA 2Managing SOX in the age of SOA 3Managing SOX in the age of SOA 4
... Since the Flp-In System creates isogenic ... equivalent levels of the protein of ... in Figure 3. In this experiment ... (CAT) was subcloned into pcDNA5/FRT and ...
For the quantitative determination of cyclic AMP (cAMP) concentrations in cell culture supernates, serum, plasma, saliva, urine and cell lysates....
SHEEP ANTI BOVINE LACTOPEROXIDASE...
Fast, sensitive quantitation of total MEK 1/2 concentration....
Biology Products: