Navigation Links
Bright lights: Mystery of glowing antibody solved by Scripps research scientists
Date:2/28/2008

n observed for stilbene and was totally unexpected, especially since stilbene is basically the E. coli of photochemistry, as it is extremely well characterized and understood.

The fact that the EP2-19G2-stilbene complex emits a bright luminescence was first described in a Scripps Research study published in the October 13, 2000 edition (Volume 290, Number 5490) of the journal Science. The underlying mechanism had remained unknown until now.

A Perfect Match, A Perfect Storm

When EP2-19G2 binds stilbene, the antibody itself coordinates the joining or stacking of stilbene with a deeply buried tryptophan residue at the active site. In other fluorescent antibodies, such a stacking interaction does not exist. After exposure to UV, electron transfer between stilbene and tryptophan occurs deep within the 19G2 protein matrix.

Kim Janda, who is Ely R. Callaway, Jr. Chair in Chemistry, member of The Skaggs Institute for Chemical Biology, and director of the Worm Institute for Research and Medicine at Scripps Research, led the initial research into EP2-19G2 and was part of the new study. He described the importance of the combination this way: This may be a perfect molecular storm. When the tryptophan residue and stilbene are matched in EP2-19G2, this perfect alignment creates a different luminescence pathway than is seen in other related antibodies.

Unlike in other complexes, this antibody reacts with stilbene in the excited state. Antibody EP2-19G2 is deeply penetrated by the stilbene molecule and the special constellation of the stilbene-tryptophan pairing sets this antibody apart from other antibody-stilbene complexes, where binding merely enhances stilbene fluorescence by inhibiting photoisomerization, the dominant nonradiative decay pathway of stilbene in solution.

In fact, the unusual binding mode of stilbene in EP2-19GE2 is enabled by a non-canonical interface of the variable heavy and light chains of the anti
'/>"/>

Contact: Mika Benedyk
mbenedyk@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

1

GOOD

Page: 1 2 3

Related biology news :

1. Researchers set new record for brightness of quantum dots
2. Has the mystery of the Antarctic ice sheet been solved?
3. UCLA researchers solve decade-old mystery
4. Ancient mystery solved
5. ASU professor helps solve mystery of glassy water
6. 480-million-year-old fossil sheds light on 150-year-old paleontological mystery
7. Time-sharing tropical birds key to evolutionary mystery
8. Cassini on the trail of a runaway mystery
9. Magnetic snakes control fluids, gravity-defying droplets, and solving a dragonfly mystery
10. Men shed light on the mystery of human longevity, study finds
11. Folate mystery finally solved
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/16/2009)... Hill, CA Tom Bowman, an expert in communicating ... Global Change, has developed a series of graphics that ... Climate Change (IPCC) report for public audiences. These new ... they need to make informed decisions about climate change ... as a means to clearly and accurately communicate findings ...
(Date:12/16/2009)... -- DigitalPersona, Inc., a leader in fingerprint ... agreement with IBM to deploy a new ... on IBM SurePOS 500 retail systems. The ... to more easily and quickly identify users ... to the individuals who perform them. ...
(Date:12/16/2009)... Texas, Dec. 8, 2009 National ... in industrial measurement and control, and DENSO ... automation using robotics technology, today announced their ... technology with DENSO robotic arms. The collaboration ... research and flexible manufacturing applications. In today,s ...
Breaking Biology News(10 mins):Bowman creates graphic translation of climate change data 2DigitalPersona Fingerprint Sensor Technology Ships on IBM SurePOS 500 Retail Systems 2National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 2National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 3National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 4National Instruments and DENSO Robotics Collaborate to Address New Applications in Industrial Robotics 5RESPONSE to the FDA and Public Health Experts Findings on Electronic Cigarettes 52613 1RESPONSE to the FDA and Public Health Experts Findings on Electronic Cigarettes 52613 2Pennsylvanians With Disabilities Call for an End to Institution Only Options 52611 1Pennsylvanians With Disabilities Call for an End to Institution Only Options 52611 2Sen Tom Daschle Rep Jason Altmire Healthcare Experts Tout Homecare as Cost Effective Solution in the Healthcare Reform Process 52609 1Sen Tom Daschle Rep Jason Altmire Healthcare Experts Tout Homecare as Cost Effective Solution in the Healthcare Reform Process 52609 2Sen Tom Daschle Rep Jason Altmire Healthcare Experts Tout Homecare as Cost Effective Solution in the Healthcare Reform Process 52609 3
... FAIRFAX, Va.Nearly 5,300 physicians, scientists and allied health ... Interventional Radiology,s 34th Annual Scientific Meeting March 7-12 ... conferences are scheduled for 9-10:30 a.m. on both ... Room 15A. , The Annual Scientific Meeting offers ...
... is launching a large-scale collaboration to develop a ... range of organizations in climate and energy policy ... John D. and Catherine T. MacArthur Foundation supports ... Economic and Resource Trajectories for Humankind). Additional internal ...
... emissions from fossil fuel combustion are now available on ... NASA and the U.S. Department of Energy through the ... emissions by the hour, geographic region, and fuel type. ... University in West Lafayette, Ind., integrated seven primary data ...
Other Biology News:Register now for the Society of Interventional Radiology's 34th Annual Scientific Meeting 2MacArthur Foundation awards planning grant to improve decision-making in energy policy 2MacArthur Foundation awards planning grant to improve decision-making in energy policy 3NASA-funded carbon dioxide map of US released on Google Earth 2
(Date:12/17/2009)... in 2010, puts healthcare cost control at the head of the ... , ... New York, NY (Vocus) December 17, 2009 -- ... in 2010, puts healthcare cost control at the ... ahead. , , , , ,“Healthcare typically lags trends in the ...
(Date:12/17/2009)... Line Screening allows users to easily find and schedule an ... at a health screening, share comments, view personal stories, and ... The health screening company’s new site will improve ... ... Cleveland, OH (PRWEB) December 17, 2009 -- Visitors to ...
(Date:12/17/2009)... TOLEDO will be exhibiting the latest particle size distribution technologies, ... Pharmaceutical Scientists’ Annual Meeting, AAPS 2010. , ... ... be exhibiting at the American Association of Pharmaceutical Scientists’ ... 14-18, 2010. Visit Booth 612 at AAPS 2010 to ...
(Date:12/16/2009)... passed as fact among historians, journalists and politicians, and ... floor of the U.S. Congress: British forces used chemical ... , But that claim has never been fully squared ... historian at Colgate University. According to Douglas,s research, forthcoming ... Modern History , no such incident ever occurred. ...
Breaking Biology Technology:Squeezing Costs out of Healthcare Tops PricewaterhouseCoopers' List of Health Industry Issues in 2010, 2Squeezing Costs out of Healthcare Tops PricewaterhouseCoopers' List of Health Industry Issues in 2010, 3Squeezing Costs out of Healthcare Tops PricewaterhouseCoopers' List of Health Industry Issues in 2010, 4Squeezing Costs out of Healthcare Tops PricewaterhouseCoopers' List of Health Industry Issues in 2010, 5Website Redesign Enhances Global Health Screening Service's Online Presence 2Website Redesign Enhances Global Health Screening Service's Online Presence 3Particle Size Distribution Technologies at American Association of Pharmaceutical Scientists (AAPS) Annual Meeting 2010 2Despite claims, UK did not gas Iraqis in the 1920s, new research finds 2Despite claims, UK did not gas Iraqis in the 1920s, new research finds 3Despite claims, UK did not gas Iraqis in the 1920s, new research finds 4
... online clothing retailer, introduced Thursday an updated version of My ... out of online shopping. , ,The new My Virtual Model ... that fit correctly by modeling garments virtually. It now provides ... resembles the customer. , ,Our customers tell us one of ...
... Wis. -- Third Wave Technologies Inc. ... platform has been successfully extended to chromosomal analysis, ... , ,In conjunction with this important extension of ... it is providing Invader analyte-specific reagents (ASRs) for ...
... Eau Claire, Wis. -- At a Grow Wisconsin forum ... Technologies of Chippewa Falls will receive $250,000 in ... Technologies create 214 jobs, retain 751 jobs at its ... equipment. , ,TTM Technologies produces complex printed computer circuit ...
Other Biology Technology: