Navigation Links
Bright lights: Mystery of glowing antibody solved by Scripps research scientists
Date:2/28/2008

n observed for stilbene and was totally unexpected, especially since stilbene is basically the E. coli of photochemistry, as it is extremely well characterized and understood.

The fact that the EP2-19G2-stilbene complex emits a bright luminescence was first described in a Scripps Research study published in the October 13, 2000 edition (Volume 290, Number 5490) of the journal Science. The underlying mechanism had remained unknown until now.

A Perfect Match, A Perfect Storm

When EP2-19G2 binds stilbene, the antibody itself coordinates the joining or stacking of stilbene with a deeply buried tryptophan residue at the active site. In other fluorescent antibodies, such a stacking interaction does not exist. After exposure to UV, electron transfer between stilbene and tryptophan occurs deep within the 19G2 protein matrix.

Kim Janda, who is Ely R. Callaway, Jr. Chair in Chemistry, member of The Skaggs Institute for Chemical Biology, and director of the Worm Institute for Research and Medicine at Scripps Research, led the initial research into EP2-19G2 and was part of the new study. He described the importance of the combination this way: This may be a perfect molecular storm. When the tryptophan residue and stilbene are matched in EP2-19G2, this perfect alignment creates a different luminescence pathway than is seen in other related antibodies.

Unlike in other complexes, this antibody reacts with stilbene in the excited state. Antibody EP2-19G2 is deeply penetrated by the stilbene molecule and the special constellation of the stilbene-tryptophan pairing sets this antibody apart from other antibody-stilbene complexes, where binding merely enhances stilbene fluorescence by inhibiting photoisomerization, the dominant nonradiative decay pathway of stilbene in solution.

In fact, the unusual binding mode of stilbene in EP2-19GE2 is enabled by a non-canonical interface of the variable heavy and light chains of the anti
'/>"/>

Contact: Mika Benedyk
mbenedyk@scripps.edu
858-784-2052
Scripps Research Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Researchers set new record for brightness of quantum dots
2. Has the mystery of the Antarctic ice sheet been solved?
3. UCLA researchers solve decade-old mystery
4. Ancient mystery solved
5. ASU professor helps solve mystery of glassy water
6. 480-million-year-old fossil sheds light on 150-year-old paleontological mystery
7. Time-sharing tropical birds key to evolutionary mystery
8. Cassini on the trail of a runaway mystery
9. Magnetic snakes control fluids, gravity-defying droplets, and solving a dragonfly mystery
10. Men shed light on the mystery of human longevity, study finds
11. Folate mystery finally solved
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/23/2014)... Huntington,s disease, most in their mid-thirties and forties, ... behavioral decline within two decades. "Mutant" protein clusters, ... disease, have been the primary focus of therapies ... new research from Prof. Gerardo Lederkremer and Dr. ... Cell Research and Immunology, in collaboration with Prof. ...
(Date:4/23/2014)... Valley, NY. (Apr. 23 2014) A ... neural stem cells (hNSCs) into the brains of ... after 22 and 24 months found that the ... and did not cause tumors. , The study ... Cell Transplantation but is currently freely ...
(Date:4/23/2014)... trope that the likelihood of an accurate group decision ... hold up when a collective faces a variety of ... Instead, Princeton University researchers report that smaller groups actually ... may become excessively focused on only certain pieces of ... what is known about collective intelligence, or the "wisdom ...
Breaking Biology News(10 mins):On the defensive 2On the defensive 3Study finds long-term survival of human neural stem cells transplanted into primate brain 2Too many chefs: Smaller groups exhibit more accurate decision-making 2Too many chefs: Smaller groups exhibit more accurate decision-making 3
... COLD SPRING HARBOR, N.Y. Combining ... with the versatility of lentiviral vectors gives researchers ... function both in vivo and in vitro. ... Protocols ( www.cshprotocols.org/TOCs/toc8_08.dtl ) features a pair ...
... parasite has waged a successful guerrilla war against the human ... Journal of Biological Chemistry has exposed one of ... which may aid in future drug development. , Malaria parasites ... their human hosts the parasites first set up shop in ...
... Cambridge, UK, 04 August 2008 - Disease Models & ... focus on translational research and the use of model organisms ... Biologists, DMM is a highly anticipated biomedical research ... and online today at http://DMM.biologists.org . DMM ...
Cached Biology News:Research exposes new target for malaria drugs 2Building bridges between the clinic and the laboratory 2
(Date:1/15/2014)... Scottsdale, Arizona (PRWEB) January 15, 2014 ... growth for Scottsdale’s Brain State Technologies®. They saw continued ... University Medical Center who were awarded a $1 million ... papers published in “Brain and Behavior” a peer reviewed ...
(Date:1/15/2014)... TAIPEI , Jan. 15, 2014 TaiGen Biotechnology ... an exclusive agreement with R-Pharm, a leading Russian pharmaceutical ... in the Russian Federation , ... Commonwealth Independent States (CIS). Nemonoxacin is a novel antibiotic for ...
(Date:1/14/2014)... January 14, 2014 Histogen, Inc., a ... of cells grown under simulated embryonic conditions, today announced ... with Suneva Medical, Inc. for physician-dispensed aesthetic products containing ... , This agreement is an amendment to the existing ...
(Date:1/14/2014)... NY (PRWEB) January 14, 2014 EquitiesIQ, ... Alliqua, Inc. (OTCQB: ALQA). Alliqua is an emerging biomedical ... serve the wound care market. , Free report download: ... restructured with a seasoned management team and Board, which ...
Breaking Biology Technology:Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 2Dynamic Innovative Technology Showcased at Scottsdale Company’s Open House 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 2Histogen and Suneva Medical Expand License for Cell Conditioned Media-based Aesthetic Products Internationally 3EquitiesIQ Initiates Coverage of Alliqua, Inc. 2EquitiesIQ Initiates Coverage of Alliqua, Inc. 3
... , SAN DIEGO, Sept. 8 eBioscience Corporation, ... today the acquisition of NatuTec GmbH, a leading distributor of high-quality ... , , "For the past 10 years, NatuTec ... Todd R. Nelson, CEO of eBioscience. "The NatuTec acquisition provides ...
... , LAIYANG, China, Sept. 8 ... "Company", "Jiangbo"), a,U.S. pharmaceutical company with its principal operations ... announced guidance for,fiscal year 2009. The Company expects ... million in revenue and operating income of $40 to ...
... , , RICHMOND, Calif., ... Institute used zinc finger nucleases (ZFNs) designed by Sangamo BioSciences, ... modify the genomes of human embryonic stem cells (hESCs) and ... useful tools in drug discovery and development. Scientists also ...
Cached Biology Technology:eBioscience Corporation Acquires NatuTec GmbH 2Jiangbo Pharmaceuticals Reaffirms Guidance for Fiscal 2009 2Sangamo's Zinc Finger Nuclease Technology Used to Efficiently Modify Human Stem Cells 2Sangamo's Zinc Finger Nuclease Technology Used to Efficiently Modify Human Stem Cells 3Sangamo's Zinc Finger Nuclease Technology Used to Efficiently Modify Human Stem Cells 4
... The INK4a-ARF locus encodes two unrelated proteins ... p14^ARF^ arrests the cell cycle in a ... promotes the rapid degradation of mdm2 proteinrequired ... and accumulation. This interaction is mediated ...
... CLS number is a new ... match Cornings product number. If ... order under the old Sigma-Aldrich ... service for assistance. Mfr ...
... Insulin exerts its ... the insulin receptor, a ... of two Alpha subunits ... arranged in the following ...
... is a new product number, ... product number. If showing no ... the old Sigma-Aldrich number (Z37,208-0) ... assistance. Mfr Desig: Corning ...
Biology Products: