Navigation Links
Breakthrough study opens door to broader biomedical applications for Raman spectroscopy
Date:2/19/2013

Amsterdam, NL, 19 February 2013 Raman spectroscopy has enabled incredible advances in numerous scientific fields and is a powerful tool for tissue classification and disease recognition, although there have been considerable challenges to using the method in a clinical setting. Scientists have now demonstrated the advantages of wavelength-modulated Raman spectroscopy, opening the door to wider biomedical and clinical applications such as real-time assessment of tissues during surgery. This study is published in Biomedical Spectroscopy and Imaging.

The inelastic scattering of light from any sample is called the Raman effect, named for the Nobel prize-winner C.V. Raman. It yields a molecular fingerprint related to the intrinsic composition of the sample. With the advent of lasers for excitation, this analytical technique has been applied in many disciplines from mineral investigations to protein structure determination and single cell studies. The technique enables cancerous lesions, which are accompanied by changes in chemical composition compared to normal tissue, to be detected as a vibrational spectroscopic fingerprint. However, there are considerable challenges to using the method in a clinical setting because factors such as ambient light, background fluorescence, and 'etaloning' (a phenomenon that degrades the performance of thinned, back-illuminated charge-coupled devices) can hinder the interpretation of images. Pre-processing the data is prone to introduce artefacts and seriously hamper a classification.

Scientists from St. Andrews (UK) and Jena (Germany) have now demonstrated that wavelength-modulated Raman spectroscopy, an alternative to standard Raman spectroscopy with monochromatic excitation, overcomes these key problems. In this study they describe how to record Raman signals against a high auto-fluorescence background by studying liver tissue and record spectra of Paracetamol tablets in ambient light.

Corresponding author Christoph Krafft, PhD, of the Institute of Photonic Technology, Jena, Germany explains: 'The principle of our implementation of wavelength-modulated Raman spectroscopy is that fluorescence emission, ambient light, and system transmission function do not significantly vary, whereas the Raman signals do vary upon multiple wavelength excitation with small wavelength shifts. In turn this leads us to 'cleanly' extract the Raman signature even in the presence of such factors. In the current work, we developed a hardware-based approach to suppress confounding factors in Raman spectra that requires a minimum of pre-processing and offers further unsurpassed advantages."

Editor-in-Chief of Biomedical Spectroscopy and Imaging, Parvez Haris, CChem, FRSC, FRSPH, adds: "This work represents a significant step beyond current Raman microscopy that breaks completely new ground. Raman analysis for biomedicine is at a crucial juncture where there is worldwide recognition that it is on the verge of potential acceptance by the wider community and clinical practice if key issues, such as the ones the authors have raised, can be overcome.

"The straightforward nature of the technique means that biologists and researchers at the life sciences interface can benefit immediately from the advantages of the novel method," he concludes.


'/>"/>

Contact: Esther Mateike
e.mateike@iospress.nl
31-206-883-355
IOS Press
Source:Eurekalert

Related biology news :

1. Explosive breakthrough in research on molecular recognition
2. Stem cell breakthrough could lead to new bone repair therapies on nanoscale surfaces
3. A Spanish breakthrough allows the electroporation of cell cultures for less than 1 Euro
4. Recent breakthroughs in cocoa flavanol research discussed by European research consortium and expert panel
5. Breakthrough: How salt stops plant growth
6. Genetic sequencing breakthrough to aid treatment for congenital hyperinsulinism
7. Plant organ development breakthrough
8. USDA scientists and cooperators sequence the wheat genome in breakthrough for global food security
9. Major breakthrough in deciphering bread wheats genetic code
10. Research breakthrough selectively represses the immune system
11. Could chloroplast breakthrough unlock key to controlling fruit ripening in crops?
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:1/6/2017)... 2017  Privately-held CalciMedica, Inc., announced that it ... of a novel calcium release-activated calcium (CRAC) channel ... Acute pancreatitis, sudden painful inflammation of ... can be very serious.  In severe cases it can ... hospital stays, time in the ICU and substantial ...
(Date:1/4/2017)... VEGAS , Jan. 4, 2017  CES ... performance biometric sensor technology, today announced the launch ... sensor systems, the highly-accurate biometric sensor modules ... biometric technology, experience and expertise. The two ... Benchmark designed specifically for hearables, and Benchmark BW2.0, ...
(Date:12/20/2016)... Dec. 20, 2016 The rising popularity ... and leasing is stoking significant interest in keyless ... technology, Bluetooth low energy (BLE), biometrics and near-field ... next wave of wireless technologies in the automotive ... to advanced access systems opens the market to ...
Breaking Biology News(10 mins):
(Date:1/19/2017)... -- WuXi AppTec, a leading global pharmaceutical, biotechnology ... today announced that it has acquired HD Biosciences ... contract research organization (CRO). After completion of acquisition, ... WuXi, and will continue to focus on growing ... acquisition will further strengthen WuXi,s R&D capability from ...
(Date:1/19/2017)... , Jan. 19, 2017  ArmaGen, Inc., ... Schmidt , Ph.D., as chief executive officer, as ... directors. Dr. Schmidt brings to ArmaGen more than 17 ... research and development of biotherapeutics and pharmaceuticals. ... with the diverse experience and skillset necessary to ...
(Date:1/19/2017)... , ... January 19, 2017 , ... ... with services spanning the full spectrum of drug and device development, and ... to pharma/device companies and clinicians, today announced Verified Clinical Trials (VCT) ...
(Date:1/18/2017)... OR (PRWEB) , ... January 18, 2017 , ... ... that provide essential device-to-computer interconnect using USB or PCI Express, announced the ZEM5310 ... Cyclone V E FPGA into a compact business-card sized form factor suitable for ...
Breaking Biology Technology: