Navigation Links
Breakthrough made at Max F. Perutz Laboratories
Date:5/8/2014

This news release is available in German.

Researchers at the Max F. Perutz Laboratories (MFPL) of the University of Vienna and the Medical University of Vienna made a breakthrough for the Platynereis model system, as they describe the first method for generating specific and inheritable mutations in the species. The method, in combination with other tools, now places this marine bristle worm in an excellent position to advance research at the frontiers of neurobiology, chronobiology, evolutionary developmental biology and marine biology. The study and a review on Platynereis dumerilii genetic methods were chosen by the renowned journal Genetics as one of the May 2014 Highlights and also got the cover image.

Many fascinating biological phenomena, of which we currently have little to no molecular understanding, can be observed in the tiny marine bristle worm Platynereis dumerilii. It displays a slow rate of evolution, which permits analyses of ancestral genes and cell types, possesses a vertebrate-type hormonal system, as well as the ability to regenerate large pieces of its body. Furthermore, its reproductive timing is controlled by multiple timers a feature likely to be common to many other organisms. These characteristics make it an ideal model for evolutionary studies as well as for chronobiology, amongst other research fields. However, dissecting Platynereis gene function in vivo had remained challenging due to a lack of available tools.

TALENs as a new tool to engineer targeted modifications in Platynereis genes

To address this need, scientists from the Max F. Perutz Laboratories (MFPL) and the Research Platform "Marine Rhythms of Life" of the University of Vienna and supported by the VIPS (Vienna International Postdoctoral program) have now established customized transcriptional activator-like effector nucleases (TALENs) as a tool to engineer targeted modifications in Platynereis genes. These tailored enzymes bind specific DNA sequences and "cut" the genome at these locations. The repair mechanisms of the cell promptly repair the damage, however small errors in the form of insertions and deletions can be introduced during the repair process. The result is the generation of small mutations that render the protein product of the gene non-functional allowing the generation of the first-ever Platynereis mutants.

Future directions

The researchers found out that the induced mutations are heritable, demonstrating that TALENs can be used for generating mutant lines in this bristle worm. "This new tool opens the door for detailed in vivo functional analyses in Platynereis and can also facilitate further technical developments. For example, we hope to use TALENs to insert fluorescent reporter genes into the genome. In this way we can study how gene expression is regulated across the entire lifecycle", explains first author Stephanie Bannister, VIPS Postdoc in Florian Raible's group at the Department of Microbiology, Immunobiology and Genetics at the University of Vienna. She spearheaded the establishment of the technique. "In addition, we have provided a streamlined workflow that can serve as a template for the establishment of TALEN technology in other non-conventional and emerging model organisms", Stephanie Bannister adds.


'/>"/>

Contact: Lilly Sommer
lilly.sommer@mfpl.ac.at
43-142-772-4014
University of Vienna
Source:Eurekalert  

Related biology news :

1. Major breakthrough in stem cell manufacturing technology
2. New invasive species breakthrough sparks interest around the world
3. A micro-muscular breakthrough
4. Scripps oceanography researchers engineer breakthrough for biofuel production
5. Breakthrough in adult heart repair
6. Breakthrough by Temple researchers could lead to new treatment for heart attack
7. Breakthrough research produces brighter, more efficiently produced lighting
8. ASU, Georgia Tech create breakthrough for solar cell efficiency
9. NTU scientists make breakthrough solar technology
10. Research breakthrough: Impaired autophagy associated with age-related macular degeneration
11. Scientific breakthrough reveals how vitamin B12 is made
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Breakthrough made at Max F. Perutz Laboratories
(Date:4/5/2017)... April 5, 2017 Today HYPR Corp. ... the server component of the HYPR platform is officially ... the end-to-end security architecture that empowers biometric authentication across ... has already secured over 15 million users across the ... of connected home product suites and physical access represent ...
(Date:4/4/2017)... NEW YORK , April 4, 2017   ... solutions, today announced that the United States Patent and ... The patent broadly covers the linking of an iris ... the same transaction) and represents the company,s 45 th ... our latest patent is very timely given the multi-modal ...
(Date:3/30/2017)... 2017  On April 6-7, 2017, Sequencing.com will host ... hackathon at Microsoft,s headquarters in Redmond, ... on developing health and wellness apps that provide a ... Genome is the first hackathon for personal genomics ... companies in the genomics, tech and health industries are ...
Breaking Biology News(10 mins):
(Date:10/11/2017)... ... October 11, 2017 , ... At its national ... Dr. Suneel I. Sheikh, the co-founder, CEO and chief research scientist of Minnesota-based ... selected for membership in ARCS Alumni Hall of Fame . ASTER Labs ...
(Date:10/11/2017)... a leading provider of patient support solutions, has announced the ... which will launch this week. The VMS CNEs will address ... enhance the patient care experience by delivering peer-to-peer education programs ... to help women who have been diagnosed and are being ... ...
(Date:10/10/2017)... ... , ... San Diego-based team building and cooking events company, Lajollacooks4u, has unveiled ... bold new look is part of a transformation to increase awareness, appeal to new ... , It will also expand its service offering from its signature gourmet cooking classes ...
(Date:10/10/2017)... Calif. , Oct. 10, 2017 SomaGenics ... from the NIH to develop RealSeq®-SC (Single Cell), expected ... for profiling small RNAs (including microRNAs) from single cells ... Program highlights the need to accelerate development of approaches ... "New techniques for measuring levels ...
Breaking Biology Technology: