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Breakthrough lights way for RNA discoveries
Date:7/29/2011

n further. They also developed several other RNA-fluorophore pairs, in addition to Spinach, that each emit a different fluorescent color, just as GFP has been evolved to exhibit a palette of colors that helps researchers track many proteins at once. Whereas GFP derivatives are often named after fruits, the Weill Cornell researchers named their RNA mimics of GFP after vegetables -- Spinach, Carrot and Radish.

The Weill Cornell investigators have already begun to use Spinach to track non-coding RNAs in cells. "Our laboratory has been very interested understanding why defects in RNA trafficking and translocation lead to developmental disorders in children, such as mental retardation," says Dr. Jaffrey. Using Spinach, they were able to watch as a non-coding RNA, fluorescing green, rapidly clusters in response to cellular stress. "We expect that Spinach will provide new insights into RNA trafficking in cells, and how this is affected in medical disorders," he says.

"There is still a lot of mystery surrounding RNA in biology. Fluorescent labeling and imaging has proved to be a powerful tool for scientists in the past, and we are hoping that Spinach too will be a tool that helps accelerate scientific discovery," says Dr. Paige.


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Contact: Andrew Klein
ank2017@med.cornell.edu
212-821-0560
New York- Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College
Source:Eurekalert

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