Navigation Links
Boston University reseachers develop faster, cheaper DNA sequencing method
Date:12/20/2009

s that govern the larger world don't necessarily apply. They made a counterintuitive discovery: the longer the DNA strand, the more quickly it found the pore opening.

"That's really surprising," Meller said. "You'd expect that if you have a longer 'spaghetti,' then finding the end would be much harder. At the same time this discovery means that the nanopore system is optimized for the detection of long DNA strands -- tens of thousands basepairs, or even more. This could dramatically speed future genomic sequencing by allowing analysis of a long DNA strand in one swipe, rather than having to assemble results from many short snippets.

"DNA amplification technologies limit DNA molecule length to under a thousand basepairs," Meller added. "Because our method avoids amplification, it not only reduces the cost, time and error rate of DNA replication techniques, but also enables the analysis of very long strands of DNA, much longer than current limitations."

With this knowledge in hand, Meller and his team set out to optimize the effect. They used salt gradients to alter the electrical field around the pores, which increased the rate at which DNA molecules were captured and shortened the lag time between molecules, thus reducing the quantity of DNA needed for accurate measurements. Rather than floating around until they happened upon a nanopore, DNA strands were funneled into the openings.

By boosting capture rates by a few orders of magnitude, and reducing the volume of the sample chamber the researchers reduced the number of DNA molecules required by a factor of 10,000 from about 1 billion sample molecules to 100,000.


'/>"/>

Contact: Mike Seele
mseele@bu.edu
617-353-9766
Boston University College of Engineering
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Grant brings real-world science to Boston classrooms
2. Boston University scientists first to see RNA network in live bacterial cells
3. Boston University engineer to use $2.5 million NIH grant to cells reaction to physical force
4. Boston University biomedical engineers teach bacteria to count
5. Boston College chemistry professor Udayan Mohanty receives a 2009 Guggenheim Fellowship
6. At Boston symposium, NARSAD researchers report on genes and family traits
7. Software developed by Boston College lab delivers speed and accuracy to genome research
8. Singapores BIOPOLIS and FUSIONOPOLIS on stage in Boston this week
9. DHS Begins Collecting 10 Fingerprints From International Visitors at Boston Logan International Airport
10. Boston College profs study oxidative stress subcellular to discover its role in diseases
11. Boston University biomedical engineers find chink in bacterias armor
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Boston University reseachers develop faster, cheaper DNA sequencing method
(Date:8/21/2014)... use a previously unknown process for harvesting energy and ... a Penn State University scientist has discovered. The discovery ... plant growth, harvesting energy from the Sun, and understanding ... and other lakes worldwide. A paper describing the discovery ... edition of the journal Science on 21 ...
(Date:8/21/2014)... aimed at improving the food intake, health and quality ... is among three projects at the University of Waterloo ... Canadian Institutes of Health Research (CIHR). , Professor ... at Waterloo, and a Schlegel research chair in nutrition ... investigate why many Canadians living in long-term care homes ...
(Date:8/21/2014)... water window? It consists of radiations in the 3.3 ... the water in biological tissues. New theoretical findings show ... the water window. These could be the basis of ... the biological samples or to be used in high-precision ... mechanism needed to efficiently generate the harmonic radiationswhich are ...
Breaking Biology News(10 mins):Hot-spring bacteria reveal ability to use far-red light for photosynthesis 2Hot-spring bacteria reveal ability to use far-red light for photosynthesis 3Grants will fund landmark aging research at Waterloo 2Water window imaging opportunity 2
... Spanish . U.S. Department of ... amending crop soils with manure also can help restore ... acres of land with little or no vegetation, once ... of southwestern Missouri, southeastern Kansas, and northeastern Oklahoma. The ...
... Sons, Inc., has announced a new partnership between the British ... Ecology and Evolution . This latest partnership brings the number ... 16. Eleven of these partner titles are ranked in the ... (ISI). BES journals will join other high impact titles ...
... Calif. Assassin bugs, so named because these insects lie ... precision, are found all over the world. Nearly 140 species ... around the mouth, they are also called kissing bugs. ... neglected tropical disease that imposes an economic burden on society. ...
Cached Biology News:Minimizing mining damage with manure 2British Ecological Society partners with Wiley open access journal Ecology and Evolution 2A clearer picture of how assassin bugs evolved 2A clearer picture of how assassin bugs evolved 3
(Date:8/21/2014)... 21, 2014  Decision Resources Group finds that ... and Mexico currently ... newly approved agents, the anticipated label extension of ... launch of emerging biologics will substantially increase the ... emerging IL-5 inhibitors will introduce a new dynamic to ...
(Date:8/21/2014)... Aug. 21, 2014  China Cord Blood Corporation (NYSE: ... to release financial results for the first quarter of ... market close in the US.  The ... ET on Friday, August 29, 2014 to discuss its ... Company,s recent developments, followed by a question and answer ...
(Date:8/21/2014)... 2014 Ontotext S4 , The ... Ontotext . Now the same enterprise hardened text ... and unstructured data is available to start-ups and mid-size ... technology. Organizations that do not have resources to evaluate ... use S4 since there is no need for on ...
(Date:8/21/2014)... August 21, 2014 On Wednesday of last ... to use Redox Signaling molecules, became available for purchase in ... , "The way that RENU 28 works is, if you ... down of the rate of cellular renewal within your body. ... increasing of that rate of cellular renewal. What RENU 28 ...
Breaking Biology Technology:Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 2Asthma Agents Currently Face a Suboptimal Coverage Environment in Brazil and Mexico, and as the Treatment Armamentarium Expands, Competitiveness Will Further Intensify 3China Cord Blood Corporation to Report First Quarter of Fiscal 2015 Financial Results 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 2Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 3Ontotext Releases Text Mining & Semantic Technology Running in the Cloud – Welcome to “S4? 4RENU 28 Skin Care Now Available in Australia and New Zealand 2
... scientist and university professor gets to see his work on the ... Siegel will get to watch his name scroll down the giant ... seems light years away from laboratory benches and lecture halls: Executive ... three-year labor of love for Siegel. From securing funding and hiring ...
... Sept. 9 /PRNewswire-FirstCall/ - ARIUS Research Inc., (TSX:,ARI), ... wave of,antibody therapeutics, today announced that RiskMetrics ISS ... they have no,objection to the transaction, and are ... the proposed plan of arrangement whereby Roche,will acquire ...
... Calif., Sept. 9 ThermoGenesis,Corp. (Nasdaq: KOOL ... that process and store adult stem cells, today ... 2008 more than doubled over the same period,a ... that it has signed,a distribution agreement with Celling ...
Cached Biology Technology:Professor-turned-producer learns the movie biz 2Professor-turned-producer learns the movie biz 3Professor-turned-producer learns the movie biz 4Professor-turned-producer learns the movie biz 5Arius announces leading independent proxy firm, RiskMetrics ISS Canada, recommends shareholders vote for the arrangement involving its acquisition by Roche 2Arius announces leading independent proxy firm, RiskMetrics ISS Canada, recommends shareholders vote for the arrangement involving its acquisition by Roche 3ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 2ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 3ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 4ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 5ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 6ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 7ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 8ThermoGenesis Reports Record Quarterly Revenues of $7.2 Million; Full Year Revenues Increase 31 Percent 9
... exciting new chapter to a ... Agilents 7890A GC gives you ... your lab to the next ... performance, including advanced separation capabilities, ...
... often call for critical, but time-consuming ... refines siRNA transfection protocols resulting in ... can be used to efficiently transfect ... without increased cytotoxicity. From start ...
... CaspaTag™ Pan-Caspase In Situ Assay Kit is ... caspases in cells undergoing apoptosis. The kit ... use in diagnostic or therapeutic procedures. CHEMICON®s ... a novel approach to detect active caspases. ...
... INTERFERin is a new-generation siRNA transfection ... efficiency at 1 nM siRNA in a ... with excellent cell viability. Using low siRNA ... effects observed when transfecting siRNA at higher ...
Biology Products: