Navigation Links
Boston University biomedical engineers teach bacteria to count
Date:5/28/2009

hat the organism is no longer in the environment or your body."

Collins' team designed two separate synthetic gene networks not found naturally in E. coli bacteria. Each uses a different method to make the bacteria count.

The first, the Riboregulated Transcriptional Cascade (RTC) synthetic gene network, counts by starting and stopping transcription and translation the process by which a gene's instructions are executed of a series of genes every time an event occurs. The researchers programmed the system so that after the third interruption, the network translates and transcribes the gene for a fluorescing protein, which is visible to researchers.

The second synthetic gene network, called DNA Invertase Cascade (DIC), works in an entirely different way. At the first event, such as the presence of a chemical, the first gene manufactures a protein that cannibalistically snips its own gene out of the network, flips it over and sticks it back in. Once the gene is backwards, it can no longer be transcribed but an extra snippet of DNA the researchers attached to its tail acts as a bookmark, showing protein-making machinery where to resume work. Each successive flip-over counts another event, and the fluorescing protein is activated after the third one.

In both methods, researchers can move the genetic parts in these counters around to fit their needs. The network might be extended to count to higher numbers, or additional genes for fluorescing proteins might be added, for example, to glow red when the bacteria have counted to two, and green at three. The network's counting can be linked to any periodic signal from the outside world the bacteria can detect, or to an internal event, such as a protein only expressed at one point during each cell division.

Each counter has strengths. The RTC can count quickly and works best when events happen every 20 to 30 minutes. The DIC takes longer to execute its flipping action, so works b
'/>"/>

Contact: Ronald Rosenberg
ronrosen@bu.edu
617-358-1240
Boston University Medical Center
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Boston College chemistry professor Udayan Mohanty receives a 2009 Guggenheim Fellowship
2. At Boston symposium, NARSAD researchers report on genes and family traits
3. Software developed by Boston College lab delivers speed and accuracy to genome research
4. Singapores BIOPOLIS and FUSIONOPOLIS on stage in Boston this week
5. DHS Begins Collecting 10 Fingerprints From International Visitors at Boston Logan International Airport
6. Boston College profs study oxidative stress subcellular to discover its role in diseases
7. Boston University biomedical engineers find chink in bacterias armor
8. University of Florida study provides insight into evolution of first flowers
9. University of Liverpool awarded $3.3 million for genomics hub
10. University awarded £1.7M to develop nanotechnology for use in health care
11. Hebrew University researchers neutralize tumor growth in embryonic stem cell therapy
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/6/2015)... BOSTON and TELTOW, Germany ... the 2015 Siggraph Conference, SensoMotoric Instruments (SMI) ... augmented reality , based on Epson,s Moverio ... eye tracking platform. With this new solution, unprecedented quality ... multi-modal, hands-free interaction with context-sensitive displays. For the first ...
(Date:8/4/2015)... Research and ... addition of the "Digital Forensics Market ... (Computer Forensics, Network Forensics, Mobile Device Forensics, ... and Region - Global Forecast to 2020" ... By Component (Hardware, Software, and Service), Sub-Segment ...
(Date:7/31/2015)... de julho de 2015 A 10 a. ... será realizada pela BGI de 22-25 de outubro de 2015, ... A conferência está celebrando seu 10 o. aniversário ... se tornou uma das reuniões mais influentes do mundo ... mais dinâmicos, entusiastas e prazerosos. A ...
Breaking Biology News(10 mins):SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3Digital Forensics Market - The Market Represents a Compound Annual Growth Rate (CAGR) of 11.3% From 2015 to 2020 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3
... grains with solubles (DDGS) to growing pigs, many questions are ... "Previous research shows that while the amount of energy ... lower digestibility of energy in DDGS than in corn," said ... Department of Animal Sciences. "Our goal was to find out ...
... University of Pennsylvania biologists studying human reproduction ... factor to the maternal age-associated increase in aneuploidy, ... during reproductive cell division. Using naturally aging ... of reproductive life is most likely caused by ...
... who develop life-threatening bacterial infections, or septicemia, with insulin ... infection, if results of a new preliminary study can ... online ahead of print in Diabetes Care ... oxidative stress in study participants who had been injected ...
Cached Biology News:Swine researchers seek answers to fiber's low digestibility 2Why the biological clock? Penn study says aging reduces centromere cohesion, disrupts reproduction 2Insulin may reduce several inflammatory factors induced by bacterial infection 2
(Date:8/26/2015)... FRANCISCO , Aug. 26, 2015  Platform ... that it has received a $1.5M Phase II ... (NIH) to further develop its therapeutic agent to ... clinical need in end stage renal disease (ESRD) ... the National Institute of Diabetes and Digestive and ...
(Date:8/26/2015)... and BEIJING , August 26, 2015 ... Impressive Acheivements  DiaCardio wins 1 st place  ... The competition, led in Israel by venture ... startups from countries including the US, China , ... Latin America .  Impressive achievement for ...
(Date:8/26/2015)... -- After litigating and negotiating patent infringement claims ... United States patent RE43,651 (the ,651 ... the United States without any admission or concession ... As a result of the parties, settlement, the US District ... dismissed the case without prejudice. Under the ...
(Date:8/26/2015)... , ... August 26, 2015 , ... PRC Clinical, ... develop new business relationships with local sponsors in southern California. The Clinical Trial Management ... drug development processes and regulatory pathways., , Stem Cell ...
Breaking Biology Technology:Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 2Symic Biomedical Awarded $1.5MM NIH Phase II SBIR Grant to Develop its Proteoglycan Mimetic Therapeutics to Reduce Vascular Access Failures in ESRD 3Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 2Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 3Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 4Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 5Israeli Startup Takes First Place at Shengjing Global Innovation Awards 2015 6P2i Settles United States Litigation 2PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 2PRC Clinical To Attend Major Industry Conferences And Meet Sponsor Companies In The San Diego Biotech Hub 3
... - N30 Pharma (N30), a,biopharmaceutical company focused on ... target endogenous,s-nitrosothiols, today announced a licensing agreement with ... use of a s-nitrosothiol drug,candidate. Financial terms of ... extremely pleased to announce this agreement. NitroMed is ...
... 29 IDM Pharma, Inc.,(Nasdaq: IDMI ), a ... announced today that Timothy P. Walbert,president and chief executive ... Conference in San Diego on Thursday, November 1, 2007,at ... Mar. A webcast of the presentation will be ...
... Monitor Offers Both Built-in ... Quality Controls, INDIANAPOLIS, Oct. 29 Roche ... controls and optional, external,liquid controls to help non-waived ... results for patients taking,blood thinner medication. The ...
Cached Biology Technology:IDM Pharma to Present at BIOCOM Investor Conference 2IDM Pharma to Present at BIOCOM Investor Conference 3Roche Diagnostics Launches CoaguChek XS Plus System to Provide New PT/INR Point-of-Care Solution for Non-Waived Anticoagulation Services 2Roche Diagnostics Launches CoaguChek XS Plus System to Provide New PT/INR Point-of-Care Solution for Non-Waived Anticoagulation Services 3Roche Diagnostics Launches CoaguChek XS Plus System to Provide New PT/INR Point-of-Care Solution for Non-Waived Anticoagulation Services 4
... quick turnaround sequencing of individual research samples, ... copy plasmids. Agencourt's world-class, high performance sequencing ... per day. The key benefits of ... 24 48 hour sample turnaround, industry ...
A component of the Acridinium protein labeling kit....
... Life Sciences can provide custom microplate coating using advanced automated ... variety of plate formats. We can also accommodate virtually any ... of 0.5-320 L):, , ... 96 well microplate, 384 well ...
... SureLINK HRP Conjugation Kits are ... protein conjugates that are consistent and ... required to conjugate your protein or ... optimized storage buffer. Activated HRP ...
Biology Products: