Biophysicists in Bochum have discovered a diode for protons: just like the electronic component determines the direction of flow of electric current, the "proton diode" ensures that protons can only pass through a cell membrane in one direction. Water molecules play an important role here as active components of the diode. The researchers led by Prof. Dr. Klaus Gerwert (Chair of Biophysics at the RUB) were able to observe this through a combination of molecular biology, X-ray crystallography, time-resolved FTIR spectroscopy and biomolecular simulations. They report in the current international online edition of the journal Angewandte Chemie.
Protons drive the protein turbines
The proton diode plays an important role in the energy production of cells. Light-driven proton pumps - certain proteins that traverse the cell membrane - eject protons out of the cell, so that excess pressure is generated outside "much like the water pressure at a dam", explains Prof. Gerwert. Elsewhere, the protons push back into the cells to compensate the concentration gradient, and thereby drive the turbines of the cell, proteins known as ATPases. The energy thus released is converted into the universal fuel of the cells, ATP (adenosine triphosphate). "This process is a kind of archaic photosynthesis" explains Prof. Gerwert. "The light energy is ultimately converted into usable energy for the organism".
We used to believe in chance
The details of these processes are the subject of research. In particular, the role of water molecules in proteins has long been unclear. "Previously it was believed that the water molecules blundered into the proteins by chance, and fulfilled no particular function", says Gerwert. Manfred Eigen, born in Bochum in 1967, was awarded the Nobel Prize for chemistry because he was able to explain why water and ice protons are such rapid conductors. The current work shows that proteins also use precisely
|Contact: Prof. Dr. Klaus Gerwert|