Navigation Links
Blossom end rot plummets in Purdue-developed transgenic tomato
Date:5/21/2012

WEST LAFAYETTE, Ind. - The brown tissue that signals blossom end rot in tomatoes is a major problem for large producers and home gardeners, but a Purdue University researcher has unknowingly had the answer to significantly lowering occurrences of the disease sitting on a shelf for 20 years.

In the early 1990s, horticulture professor Avtar Handa developed a transgenic tomato with a thicker juice that yields 10 percent more tomato paste than parental, non-engineered tomatoes. He said large commercial producers were interested but weren't ready to bring a transgenic tomato on the market, especially with the regulatory approval process that was required. So the research findings were published and the seeds stored away.

About two years ago, researchers at the University of California-Davis asked Handa for seeds from this tomato line. They were particularly interested in Handa's observations about how the tomatoes stored calcium, as several fruit diseases are thought to be caused by calcium deficiency. In particular, blossom end rot results in dark, softened spots on the end of tomatoes.

"Calcium is difficult to move from the soil into the plant," Handa said. "It is a major problem in tomatoes and many other fruit crops."

Handa shared his seeds with Elizabeth Mitcham, a post-harvest pomologist, and Sergio Tonetto de Freitas, formerly a doctoral student and now a postdoctoral researcher, both at UC Davis. They found that Handa's tomato plants essentially allow more calcium to be free and mobile in tomato cells, significantly reducing the occurrence of blossom end rot. According to the findings, published in the early online version of The Plant Journal, about 80 percent of wild-type tomatoes will suffer from blossom end rot in conditions conducive to the disease. Under similar conditions, only 30 percent of Handa's transgenic tomatoes develop blossom end rot.

"It's a significant decrease - more than twofold," Mitcham said.

Non-engineered tomatoes produce high levels of an enzyme called pectin methylesterases, which creates free carboxylic acids in fruit cell walls. These acids bind calcium and immobilize it in the fruit, Handa said.

"If you have a lot of pectin methylesterase activity, much of the calcium in the cells becomes bound to the cell wall," Mitcham said. "That calcium is then unavailable to protect the cell membrane and prevent these physiological disorders."

Handa's strategy for producing thicker juices involved silencing pectin methylesterase production in a transgenic tomato, greatly reducing the binding sites for calcium within the fruit cell walls. That allowed the calcium to be used in other parts of the tomato's cells.

"Freed-up calcium from cell walls likely overcomes the underlying cause of blossom end rot," Handa said.

Mitcham will continue to study the mechanisms that cause blossom end rot in tomatoes, as well as how pectin methylesterases and calcium may play roles in other plant diseases thought to be caused by calcium deficiencies, including in apples, lettuce, peppers and watermelons.

Handa said this development, and the fact transgenic plants have become more common, might get tomato producers interested in the tomato genotype he developed more than 20 years ago.

"We're coming to a time when people are starting to use genetically modified crops," Handa said. "The technology is matured and dependable and ready to be used now."


'/>"/>

Contact: Brian Wallheimer
bwallhei@purdue.edu
765-496-2050
Purdue University
Source:Eurekalert  

Related biology news :

1. What genes help blossoms last longer?
2. Hybrid string blossom thinner tested in peach orchards
3. String blossom thinner proves effective across stages of bloom development
4. Blossom end rot: Transport protein identified
5. Risks involved with transgenic fish
6. Transgenic songbirds provide new tool to understand the brain
7. Yerkes researchers create first transgenic prairie voles
8. UCSF transgenic mouse mimics Parkinsons earliest symptoms
9. Transgenic corn suppresses European corn borer, saves farmers billions
10. Gene transfer from transgenic crops: A more realistic picture
11. Mutants with heterozygote disadvantage can prevent spread of transgenic animals
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Blossom end rot plummets in Purdue-developed transgenic tomato
(Date:4/5/2017)... SEATTLE , April 5, 2017  The Allen ... the Allen Cell Explorer: a one-of-a-kind portal and dynamic ... large-scale 3D imaging data, the first application of deep ... edited human stem cell lines and a growing suite ... the platform for these and future publicly available resources ...
(Date:4/3/2017)... WASHINGTON , April 3, 2017 /PRNewswire-USNewswire/ ... single-cell precision engineering platform, detected a statistically ... cell product prior to treatment and objective ... highlight the potential to predict whether cancer ... prior to treatment, as well as to ...
(Date:3/29/2017)... the health IT company that operates the largest health ... today announced a Series B investment from BlueCross BlueShield ... investment and acquisition accelerates higi,s strategy to create the ... activities through the collection and workflow integration of ambient ... secures data today on behalf of over 36 million ...
Breaking Biology News(10 mins):
(Date:6/22/2017)... ... June 22, 2017 , ... Charm Sciences, Inc. is pleased to announce ... determined to be appropriate as a screening test at dairies and farms for raw ... system, and the Charm EZ Lite system. These systems are a combination incubator and ...
(Date:6/20/2017)... ... 20, 2017 , ... HorizonScan is providing food and ingredient ... likely threat to their products at the annual IFT conference in Las Vegas, ... expo attracts over 20,000 attendees representing food science professionals from over 90 countries ...
(Date:6/20/2017)... ... 20, 2017 , ... Do More with OHAUS , With the launch of ... in the weighing industry, to extending its expertise across the entire laboratory to a ... and more, allowing for its customers to 'Do More' in the lab. ...
(Date:6/19/2017)... ... June 19, 2017 , ... EDETEK, Inc., ... today that it is launching two new additions of its award-winning cloud-based platform ... capabilities at the DIA 2017 Annual Meeting in Chicago, IL, June 19-22, 2017. ...
Breaking Biology Technology: