Navigation Links
Blossom end rot plummets in Purdue-developed transgenic tomato
Date:5/21/2012

WEST LAFAYETTE, Ind. - The brown tissue that signals blossom end rot in tomatoes is a major problem for large producers and home gardeners, but a Purdue University researcher has unknowingly had the answer to significantly lowering occurrences of the disease sitting on a shelf for 20 years.

In the early 1990s, horticulture professor Avtar Handa developed a transgenic tomato with a thicker juice that yields 10 percent more tomato paste than parental, non-engineered tomatoes. He said large commercial producers were interested but weren't ready to bring a transgenic tomato on the market, especially with the regulatory approval process that was required. So the research findings were published and the seeds stored away.

About two years ago, researchers at the University of California-Davis asked Handa for seeds from this tomato line. They were particularly interested in Handa's observations about how the tomatoes stored calcium, as several fruit diseases are thought to be caused by calcium deficiency. In particular, blossom end rot results in dark, softened spots on the end of tomatoes.

"Calcium is difficult to move from the soil into the plant," Handa said. "It is a major problem in tomatoes and many other fruit crops."

Handa shared his seeds with Elizabeth Mitcham, a post-harvest pomologist, and Sergio Tonetto de Freitas, formerly a doctoral student and now a postdoctoral researcher, both at UC Davis. They found that Handa's tomato plants essentially allow more calcium to be free and mobile in tomato cells, significantly reducing the occurrence of blossom end rot. According to the findings, published in the early online version of The Plant Journal, about 80 percent of wild-type tomatoes will suffer from blossom end rot in conditions conducive to the disease. Under similar conditions, only 30 percent of Handa's transgenic tomatoes develop blossom end rot.

"It's a significant decrease - more than twofold," Mitcham said.

Non-engineered tomatoes produce high levels of an enzyme called pectin methylesterases, which creates free carboxylic acids in fruit cell walls. These acids bind calcium and immobilize it in the fruit, Handa said.

"If you have a lot of pectin methylesterase activity, much of the calcium in the cells becomes bound to the cell wall," Mitcham said. "That calcium is then unavailable to protect the cell membrane and prevent these physiological disorders."

Handa's strategy for producing thicker juices involved silencing pectin methylesterase production in a transgenic tomato, greatly reducing the binding sites for calcium within the fruit cell walls. That allowed the calcium to be used in other parts of the tomato's cells.

"Freed-up calcium from cell walls likely overcomes the underlying cause of blossom end rot," Handa said.

Mitcham will continue to study the mechanisms that cause blossom end rot in tomatoes, as well as how pectin methylesterases and calcium may play roles in other plant diseases thought to be caused by calcium deficiencies, including in apples, lettuce, peppers and watermelons.

Handa said this development, and the fact transgenic plants have become more common, might get tomato producers interested in the tomato genotype he developed more than 20 years ago.

"We're coming to a time when people are starting to use genetically modified crops," Handa said. "The technology is matured and dependable and ready to be used now."


'/>"/>

Contact: Brian Wallheimer
bwallhei@purdue.edu
765-496-2050
Purdue University
Source:Eurekalert  

Related biology news :

1. What genes help blossoms last longer?
2. Hybrid string blossom thinner tested in peach orchards
3. String blossom thinner proves effective across stages of bloom development
4. Blossom end rot: Transport protein identified
5. Risks involved with transgenic fish
6. Transgenic songbirds provide new tool to understand the brain
7. Yerkes researchers create first transgenic prairie voles
8. UCSF transgenic mouse mimics Parkinsons earliest symptoms
9. Transgenic corn suppresses European corn borer, saves farmers billions
10. Gene transfer from transgenic crops: A more realistic picture
11. Mutants with heterozygote disadvantage can prevent spread of transgenic animals
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Blossom end rot plummets in Purdue-developed transgenic tomato
(Date:5/24/2016)... Ampronix facilitates superior patient care by providing unparalleled technology to leaders of the medical ... premium product recently added to the range of products distributed by Ampronix. ... ... ... Ampronix News ...
(Date:5/12/2016)... , May 12, 2016 WearablesResearch.com ... just published the overview results from the Q1 wave ... the recent wave was consumers, receptivity to a program ... data with a health insurance company. "We ... to share," says Michael LaColla , CEO of ...
(Date:4/28/2016)... , April 28, 2016 Infosys ... (NYSE: INFY ), and Samsung SDS, a global ... that will provide end customers with a more secure, fast ...      (Logo: http://photos.prnewswire.com/prnh/20130122/589162 ) , ... but it also plays a fundamental part in enabling and ...
Breaking Biology News(10 mins):
(Date:6/24/2016)... TOKYO , June 24, 2016  Regular discussions on ... to take place between the two entities said Poloz. ... in Ottawa , he pointed to the ... and the federal government. ... Poloz said, "Both institutions have common economic goals, why not ...
(Date:6/23/2016)... ... 2016 , ... UAS LifeSciences, one of the leading manufacturers ... Probiotics, into Target stores nationwide. The company, which has been manufacturing high quality ... list of well-respected retailers. This list includes such fine stores as Whole Foods, ...
(Date:6/23/2016)... ... June 23, 2016 , ... Charm Sciences, Inc. is ... has received AOAC Research Institute approval 061601. , “This is another AOAC-RI approval ... Bob Salter, Vice President of Regulatory and Industrial Affairs. “The Peel Plate methods ...
(Date:6/23/2016)... ... , ... STACS DNA Inc., the sample tracking software company, today announced that ... joined STACS DNA as a Field Application Specialist. , “I am thrilled that ... of STACS DNA. “In further expanding our capacity as a scientific integrator, Hays brings ...
Breaking Biology Technology: