Navigation Links
Bioengineers at University of Pennsylvania devise nanoscale system to measure cellular forces
Date:8/27/2007

University of Pennsylvania researchers have designed a nanoscale system to observe and measure how individual cells react to external forces.

By combining microfabricated cantilevers and magnetic nanowire technology to create independent, nanoscale sensors, the study showed that cells respond to outside forces and demonstrated a dynamic biological relationship between cells and their environment.

The study also revealed that cells sense force at a single adhesion point that leads not to a local response but to a remote response from the cells internal forces, akin to tickling the cells elbow and watching the knee kick.

The cell senses the force that we apply and adjusts its own internal forces to compensate, Chris Chen, an associate professor in the Department of Bioengineering in the School of Engineering and Applied Science at Penn, said. This suggests that either the cells cytoskeleton dictates the reaction or the cell organizes a biochemical response. In either instance, cells are adapting at the microscale.

The findings prove useful to more than just an understanding of the mechanics of single cells. Physical forces play a strong role in how whole tissue grows and functions. Using the Penn system, researchers could monitor for differences in how forces are sensed or generated in normal and diseased cells. This could lead to new therapeutic drug targets and to methods for modifying how cells interact with each other.

To study the cells biomechanical response to forces, Chen and his team applied force to each cell using microfabricated arrays of magnetic posts containing cobalt nanowires interspersed amongst an array of non-magnetic posts. In the magnetic field, the posts with nanowires applied an external force to cells cultured on the tops of the posts. Nonmagnetic posts acted as sensors in which traction forces in each cell were measured. Recording the traction forces in response to such force stimulation revealed two responses: a sudden loss in contractility that occurred within the first minute of stimulation or a gradual decay in contractility over several minutes.

For both types of responses, the subcellular distribution of loss in traction forces was not confined to locations near the actuated micropost or uniformly across the whole cell but instead occurred at discrete locations along the cell periphery. Together, these data suggest that cells actively adjust their internal tension to mechanical forces arising in their microenvironment and reveal an important dynamic biological relationship between external and internal forces.

Mechanical forces contribute to many cellular functions, including changes in gene expression, proliferation and differentiation.

Applying shear or tensile stresses to cells in culture, for example, can induce changes in adhesion regulation, intracellular signaling and cell function much like internal forces do. The similarities in cellular responses to external and internal forces have led to the suggestion that both types of forces may use shared mechanotransduction pathways to convert mechanical stimuli into biochemical signals. While externally applied and internally generated forces may act independently on cells, the University of Pennsylvania team postulated and then showed that they are coupled.


'/>"/>

Contact: Jordan Reese
jreese@pobox.upenn.edu
215-573-6604
University of Pennsylvania
Source:Eurekalert

Related biology news :

1. Bioengineers create stable networks of blood vessels
2. Rice bioengineers pioneer techniques for knee repair
3. University of Manchester makes made-to-measure skin and bones a reality using inkjet printers
4. New protein discovered by Hebrew University researchers
5. Next Generation Body Scanner Launched By The University Of Manchester
6. Roundup®highly lethal to amphibians, finds University of Pittsburgh researcher
7. Green catalyst destroys pesticides and munitions toxins, finds Carnegie Mellon University
8. University of Nevada, Reno research team discovers hormone that causes malaria mosquito to urinate
9. Carnegie Mellon University research reveals how cells process large genes
10. University of Delaware researchers develop cancer nanobomb
11. University of Arizona plant scientists to unravel maize genome
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/31/2016)... , March 31, 2016  Genomics firm Nabsys ... founding CEO, Barrett Bready , M.D., who returned ... of the original technical leadership team, including Chief Technology ... of Product Development, Steve Nurnberg and Vice President of ... to the company. Dr. Bready served as ...
(Date:3/22/2016)... 2016 According to ... for Consumer Industry by Type (Image, Motion, Pressure, ... & IT, Entertainment, Home Appliances, & Wearable ... 2022", published by MarketsandMarkets, the market for ... USD 26.76 Billion by 2022, at a ...
(Date:3/18/2016)... 2016 --> --> ... Manned & Unmanned Vehicles, Physical infrastructure and Perimeter Surveillance & ... the border security market and the continuing migration crisis in ... Europe has led visiongain to publish this unique ... --> defence & security companies in the border ...
Breaking Biology News(10 mins):
(Date:5/25/2016)... ... May 25, 2016 , ... Founder of ... board-certified in surgery and surgery of the hand by the National Board of ... to going above and beyond in his pursuit of providing the most comprehensive, ...
(Date:5/25/2016)... ... May 25, 2016 , ... Lady had been battling arthritis since the ... in her left knee. Lady’s owner Hannah sought the help of Dr Jeff Christiansen ... to repair her cruciate ligament and help with the pain of Lady’s arthritis. Dr ...
(Date:5/24/2016)... ... May 24, 2016 , ... Last week, Callan Capital, an integrated wealth management ... The Future of San Diego Life Science event at the Estancia La Jolla Resort ... the event with speakers Dr. Rich Heyman, former CEO of Aragon and Seragon, and ...
(Date:5/23/2016)... Zimmer Biomet Holdings, Inc. (NYSE and SIX: ZBH), ... Board of Directors has approved the payment of a quarterly ... The cash dividend of $0.24 per share ... stockholders of record as of the close of business on ... approval of the Board of Directors and may be adjusted ...
Breaking Biology Technology: