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Biochemical 'on-switch' could solve protein purification challenge
Date:10/22/2009

tentially therapeutic protein, each copy of which has a subtilisin sheath attached to it. After harvesting these proteins along with the thousands of others that grow in the cellular interior, they filter the mixture through the strainer, where the protein-sheath pairs are caught and stuck fast to the subtilisin while the rest of the mixture drains away.

At this point, the team flicks their switch. They add a bit of fluoride and the enzyme snips the bond between sheath and protein, releasing the desired protein free of almost all impurities. "The technique can conceivably be used to obtain any protein you like, and the process is repeatable, as the sheaths can be removed for another round of purification," Gallagher says. "For most proteins, the method can achieve greater than 95 percent purity at a single step."


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Contact: Chad Boutin
boutin@nist.gov
301-975-4261
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

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