Navigation Links
Biochemical 'on-switch' could solve protein purification challenge
Date:10/22/2009

tentially therapeutic protein, each copy of which has a subtilisin sheath attached to it. After harvesting these proteins along with the thousands of others that grow in the cellular interior, they filter the mixture through the strainer, where the protein-sheath pairs are caught and stuck fast to the subtilisin while the rest of the mixture drains away.

At this point, the team flicks their switch. They add a bit of fluoride and the enzyme snips the bond between sheath and protein, releasing the desired protein free of almost all impurities. "The technique can conceivably be used to obtain any protein you like, and the process is repeatable, as the sheaths can be removed for another round of purification," Gallagher says. "For most proteins, the method can achieve greater than 95 percent purity at a single step."


'/>"/>

Contact: Chad Boutin
boutin@nist.gov
301-975-4261
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Developer of advanced computing memory, father of biochemical engineering, and innovative engineering educators win highest engineering honors of 2009
2. UT Southwestern researchers disrupt biochemical system involved in cancer, degenerative disease
3. Margarita Saenz, M.D. is recipient of Genzyme/ACMGF Fellowship in Biochemical Genetics
4. Kelvin Lee winner of Biochemical Engineering Journal Young Investigator Award
5. Newly created cancer stem cells could aid breast cancer research
6. Obesity and lack of exercise could enhance the risk of pancreatic cancer
7. Finding that 1-in-a-billion that could lead to disease
8. 60 second test could help early diagnosis of common brain diseases
9. Auto immune response creates barrier to fertility; could be a step in speciation
10. Paracetamol, one of most used analgesics, could slow down bone growth
11. Drug could improve pregnancy outcomes in wider range of women with insulin resistance
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Biochemical 'on-switch' could solve protein purification challenge
(Date:8/19/2014)... in a number of ways. They have an impressive ... organs. After being struck by lightning, for example, a ... is one major downside to life as a plant: ... which they live and therefore completely at the mercy ... have developed mechanisms that enable them to rapidly adapt ...
(Date:8/19/2014)... treat pain, but its effect on pain following ... recent study reported on the Neural Regeneration ... models of an avulsion injury to the left ... pain) were given electroacupuncture stimulation at bilateral Quchi ... After electroacupuncture therapy, chronic neuropathic pain in the ...
(Date:8/19/2014)... in German . ... the metabolism of plants can switch off individual genes ... the Max Planck Institute for Plant Breeding Research in ... adopt a different approach. They identify small molecules that ... pads and prevent the downstream reactions. In their search ...
Breaking Biology News(10 mins):How steroid hormones enable plants to grow 2Researchers block plant hormone 2Researchers block plant hormone 3
... infancy has revealed that, at just four months old, ... least three different ways, according to brightness, shape, and ... new findings mean that very young infants are much ... had previously thought. The study also has implications ...
... Pacific Northwest National Laboratory scientists have used electrostatic ... spaghetti-like carbon nanotubes. , This configuration can ... blood-sugar monitoring to infectious-agent detection, said Yuehe Lin, ... Energy campus' W.R. Wiley Environmental Molecular Sciences Laboratory. ...
... strengths of a vaccine designed to fight avian influenza will ... Duke study is part of a multicenter trial that will ... to determine whether the vaccine still triggers a strong immune ... sponsored by the National Institute of Allergy and Infectious Diseases ...
Cached Biology News:Infants can organise visual information at just four months 2Infants can organise visual information at just four months 3A biosensor layered like lasagna 2Duke to test bird flu vaccine dosing 2
(Date:8/19/2014)... August 19, 2014 Sales Horizons, a ... its online sales skills training course to ... , MedTech clinical staff spend the majority of their ... standalone medical center, or physician practice – providing support ... why stop there? Sales Horizons believes clinical staff are ...
(Date:8/18/2014)... FREDERICK, Md. , Aug. 18, 2014 ... the maker of advanced consumer medical devices ... Kotak was interviewed by CEOLIVE.TV as ... The entire interview is available at ... interview Dr. Kotak explains to investors the ...
(Date:8/18/2014)... 18, 2014 According to ... Research "Life Science Reagents Market(Products- PCR Reagent Kits, ... In-Vitro Diagnostic Reagents and Others; End Users- Commercial ... Laboratories, and Forensic Laboratories)- Global Industry Analysis, Size, ... the global life science reagents market was valued ...
(Date:8/18/2014)... China is increasingly being recognized ... research industry. Clinicaltrial.gov statistics highlight the popularity of ... population; a high increase in infectious diseases; a ... pool of patients and a track record of ... China also presents challenges for the clinical research ...
Breaking Biology Technology:Sales Horizons Launches a New Online Sales Training Course for MedTech Clinical Staff to Develop Their Sales Skills 2Sales Horizons Launches a New Online Sales Training Course for MedTech Clinical Staff to Develop Their Sales Skills 3BioElectronics EVP Dr. Deepak Kotak Interviewed on CEO Live 2BioElectronics EVP Dr. Deepak Kotak Interviewed on CEO Live 3Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 2Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 3Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 4Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 5China – Land of Opportunities & Challenges for the Clinical Research Industry, New Webinar Hosted by Xtalks 2
... Manages Entire Project Information, CAMBRIDGE, England ... Corporation plc (LSE: AU. or AU.L), a,global ... today,announced that Extract Technology, a leading supplier ... biotech markets, has,implemented its Meridio records management ...
... Columbia researchers has developed a technique that controls the ... a procedure considered impossible for the past two decades. ... team deposited potassium atoms onto the surface of a ... scientists to continuously manipulate the number of electrons on ...
... Calif. Physicists at UC Riverside have made ... potential to change how information in computers can ... of electrons, a property electrons possess that makes ... help in the development of spin-based semiconductor technology ...
Cached Biology Technology:Autonomy Meridio Enables Extract Technology to Enhance Capabilities in Compliance With Manufacturing Regulations 2Autonomy Meridio Enables Extract Technology to Enhance Capabilities in Compliance With Manufacturing Regulations 3Autonomy Meridio Enables Extract Technology to Enhance Capabilities in Compliance With Manufacturing Regulations 4UBC physicists develop 'impossible' technique to study and develop superconductors 2Discovery by UC Riverside physicists could enable development of faster computers 2Discovery by UC Riverside physicists could enable development of faster computers 3
Negative Control, eluted and packaged aseptically in 20l of 10mM Tris-HCL, pH 8.0, 1 mM EDTA, 5 years at either 4°C or -20°C...
AdestaTM Tissue/Cell Preservation Medium Sterile / Non-sterile: Sterility Tested Endotoxin: ...
...
... Clone/PAD: ZMD.440. Immunogen: Synthetic peptide derived ... PDGF-D (platelet derived growth factor D iris-expressed ... which differs from mouse and rat by ... with the ~50 kDa human mouse and ...
Biology Products: