Navigation Links
Biochemical 'on-switch' could solve protein purification challenge
Date:10/22/2009

Drugs based on engineered proteins represent a new frontier for pharmaceutical makers. Even after they discover a protein that may form the basis of the next wonder drug, however, they have to confront a long-standing problem: how to produce large quantities of the protein in a highly pure state. Now, a multi-institutional research team including a biochemist at the National Institute of Standards and Technology (NIST) may have found* a new solution in an enzymatic "food processor" they can activate at will.

The team has found an efficient method of harvesting purified protein molecules by altering an enzyme that soil bacteria use to break down their food. In its natural form, this enzyme would be of little use to drug developers, but the team has modified it so that it can be activated at the desired moment. By creating essentially an "on-switch" for the enzyme's activity, the team has found a way to separate a single, desired protein from the mixture of thousands generated by a living cell, which remains biotechnology's natural protein factory of choice.

Bacteria use the enzyme, called subtilisin, as a sort of food processor: After producing it internally, they release the enzyme into the soil, where it uses a minuscule "blade" to chop up proteins into digestible pieces. Because it could damage the bacterium's interior, the blade has a protective sheath that only comes off once the enzyme has exited the cell.

"The enzyme and sheath are strongly attracted to each other. The enzyme's first act is to cut the sheath away," says NIST's Travis Gallagher. "The method takes advantage of their attraction in order to isolate the protein we want."

The team first creates many "sheathless" copies of the enzyme, which are modified to function only in the presence of a triggering molecule such as fluoride. The modified enzymes are bound to the surface of a strainer. Then the team uses engineered cells to generate mass quantities of a po
'/>"/>

Contact: Chad Boutin
boutin@nist.gov
301-975-4261
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Developer of advanced computing memory, father of biochemical engineering, and innovative engineering educators win highest engineering honors of 2009
2. UT Southwestern researchers disrupt biochemical system involved in cancer, degenerative disease
3. Margarita Saenz, M.D. is recipient of Genzyme/ACMGF Fellowship in Biochemical Genetics
4. Kelvin Lee winner of Biochemical Engineering Journal Young Investigator Award
5. Newly created cancer stem cells could aid breast cancer research
6. Obesity and lack of exercise could enhance the risk of pancreatic cancer
7. Finding that 1-in-a-billion that could lead to disease
8. 60 second test could help early diagnosis of common brain diseases
9. Auto immune response creates barrier to fertility; could be a step in speciation
10. Paracetamol, one of most used analgesics, could slow down bone growth
11. Drug could improve pregnancy outcomes in wider range of women with insulin resistance
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Biochemical 'on-switch' could solve protein purification challenge
(Date:8/6/2015)... SAN JOSE, Calif. , Aug. 6, 2015 ... the leading developer of human interface solutions, today ... and TouchPad technologies on Windows 10. Microsoft leveraged ... fundamental to the operating system. ... and drivers are fully certified with Microsoft,s Precision ...
(Date:8/4/2015)... ) today reported financial and operating results for the second quarter ... quarter contract revenue of $ 85.2 million, a ... of 26 % , Second quarter adjusted ... royalties in the current quarter , Operating cash ... to present another strong financial quarter, with all our divisions achieving ...
(Date:7/31/2015)... 2015 La 10e Conférence internationale sur ... par le BGI du 22 au 25 octobre ... Chine. La conférence célèbre son 10e ... l,ICG est devenue l,une des réunions annuelles mondiales ... et c,est aussi la plus dynamique, la plus ...
Breaking Biology News(10 mins):Synaptics Chosen for Human Interface Co-Development on Windows 10 2AMRI Announces Second Quarter 2015 Results 2AMRI Announces Second Quarter 2015 Results 3AMRI Announces Second Quarter 2015 Results 4AMRI Announces Second Quarter 2015 Results 5AMRI Announces Second Quarter 2015 Results 6AMRI Announces Second Quarter 2015 Results 7AMRI Announces Second Quarter 2015 Results 8AMRI Announces Second Quarter 2015 Results 9AMRI Announces Second Quarter 2015 Results 10AMRI Announces Second Quarter 2015 Results 11AMRI Announces Second Quarter 2015 Results 12AMRI Announces Second Quarter 2015 Results 13AMRI Announces Second Quarter 2015 Results 14AMRI Announces Second Quarter 2015 Results 15AMRI Announces Second Quarter 2015 Results 16AMRI Announces Second Quarter 2015 Results 17AMRI Announces Second Quarter 2015 Results 18AMRI Announces Second Quarter 2015 Results 19AMRI Announces Second Quarter 2015 Results 20AMRI Announces Second Quarter 2015 Results 21AMRI Announces Second Quarter 2015 Results 22AMRI Announces Second Quarter 2015 Results 23AMRI Announces Second Quarter 2015 Results 24La 10e International Conference on Genomics (ICG-10) doit s'ouvrir en octobre 2La 10e International Conference on Genomics (ICG-10) doit s'ouvrir en octobre 3
... Voter Rolls and ... National ID Cards, ... Bulletin Board: BKYI), a leader in,finger-based biometric identification and wireless public ... prepackaged and customized software,solutions, today announced that they have been awarded ...
... the topping-out ceremony for the experimental hall of PETRA III ... Hamburg. On occasion of the structural completion of the building, ... Ole von Beust visited the Helmholtz research centre DESY. Its ... in 2009. PETRA III is a milestone on the ...
... A pasture improvement research program by Dr. Dariusz Malinowski ... an alternative to winter wheat pastures. But these ... parts of the nation, said Malinowski, a Texas Agricultural ... the Mediterranean Basin of southern Europe and northern Africa. ...
Cached Biology News:BIO-key(R) and Tiger IT Awarded a Follow-on Contract for Nationwide Voter / Citizen Registration in Bangladesh 2BIO-key(R) and Tiger IT Awarded a Follow-on Contract for Nationwide Voter / Citizen Registration in Bangladesh 3BIO-key(R) and Tiger IT Awarded a Follow-on Contract for Nationwide Voter / Citizen Registration in Bangladesh 4Brilliant perspectives 2Brilliant perspectives 3Summer-dormant tall fescue grass shows promise for pasture improvements 2Summer-dormant tall fescue grass shows promise for pasture improvements 3
(Date:8/31/2015)... Research and Markets ( http://www.researchandmarkets.com/research/cbgl72/middle_east_and ) has ... Africa Biomedical Sensors Market - Growth, Trends & Forecasts ... The Middle East & Africa ... 2018 at a CAGR of 3.26% over the period ... sensors that are adaptable to the genetic formulation of ...
(Date:8/31/2015)... HAYWARD, Calif. and SHANGHAI ... Inc., a privately-held biopharmaceutical company developing next-generation antibiotics, ... Phase 2 clinical study for its lead drug ... designed to treat drug-resistant bacteria such as MRSA ... safer and better tolerated therapeutic option than currently ...
(Date:8/31/2015)... , ... August 31, 2015 , ... ... the world's most successful food and beverage processors, equipment manufacturers and leaders in ... product inspection and industrial weighing and formulation for manufacturers of bakery and snacks; ...
(Date:8/28/2015)... , August 28, 2015 ... Product (Media, Sera, Cryogenic Storage Systems, Thawing Equipment, Alarms), ... Regenerative Medicine, Clinical Trials), End User - Global Forecast ... is expected to reach USD 3,731.03 Million by 2020 ... CAGR of 11.65% between 2015 and 2020. ...
Breaking Biology Technology:Middle East & Africa Biomedical Sensors Market Report 2015 - Growth, Trends & Forecasts to 2020 2MicuRX Reports Positive Top-Line Results In Phase 2 Clinical Trial For Novel Antibiotic MRX-I In Complicated Skin And Soft Tissue Infections 2METTLER TOLEDO Hosts Food Safety Educational Center at 2015 PROCESS EXPO 2Biopreservation Market Worth 3,731.03 Million USD by 2020 2Biopreservation Market Worth 3,731.03 Million USD by 2020 3Biopreservation Market Worth 3,731.03 Million USD by 2020 4Biopreservation Market Worth 3,731.03 Million USD by 2020 5
... PITTSBURGH, Feb. 2, 2011 Forensic DNA profiling has ... people in the aftermath of mass violence and disaster, ... to identify and analyze the major ethical and policy ... (Logo: http://photos.prnewswire.com/prnh/20020422/CMULOGO) The National ...
... 2, 2011 New England Biolabs has expanded its ... generation sequencing to include NEBNext® Small RNA Sample Prep ... NEBNext Small RNA Sample Prep Set 3 for sequencing ... new and unique protocol, which results in higher yields ...
... N.M., Feb. 2, 2011 Elixir Bio-Tech Inc. (EBT), ... one of the top fifty winners of the FundingPost,s ... EBT is launching its Algae Biomass Feedstock project in ... multi-purpose feedstock with ground-breaking Proprietary and Patent Pending Technology ...
Cached Biology Technology:CMU Receives $1.2 Million NIH Grant To Develop Ethics, Policy Recommendations for Post-Conflict, Post-Disaster DNA ID Practices 2CMU Receives $1.2 Million NIH Grant To Develop Ethics, Policy Recommendations for Post-Conflict, Post-Disaster DNA ID Practices 3CMU Receives $1.2 Million NIH Grant To Develop Ethics, Policy Recommendations for Post-Conflict, Post-Disaster DNA ID Practices 4New England Biolabs Develops Novel Small RNA NGS Protocol: Reagents Improve Yield and Decrease Adaptor-Dimer Formation 2Elixir Bio-Tech Inc. Elected One of Top 50 Emerging Companies by 100 Venture Capital Funds & Angel Investors 2
EDTA, tetrasodium salt, 1 kg. Ethylene diaminetetraacetic acid.Dihydrate. Assay: 99-101% (as is).Risks: 36, 37, 38 ; Safety Precautions: 22, 36, 37. Category: Nucleotides & Enzymes & Biochemicals, Ul...
EDG-1 Western blot control Source: Cell Lysate Application: Western Blotting Control...
...
Kit containing positive control DNA all parts of the HIV-1 genome, HIV-1 negative control DNA, Primers SK38, SK39, SK19...
Biology Products: