Navigation Links
Biochemical 'on-switch' could solve protein purification challenge
Date:10/22/2009

Drugs based on engineered proteins represent a new frontier for pharmaceutical makers. Even after they discover a protein that may form the basis of the next wonder drug, however, they have to confront a long-standing problem: how to produce large quantities of the protein in a highly pure state. Now, a multi-institutional research team including a biochemist at the National Institute of Standards and Technology (NIST) may have found* a new solution in an enzymatic "food processor" they can activate at will.

The team has found an efficient method of harvesting purified protein molecules by altering an enzyme that soil bacteria use to break down their food. In its natural form, this enzyme would be of little use to drug developers, but the team has modified it so that it can be activated at the desired moment. By creating essentially an "on-switch" for the enzyme's activity, the team has found a way to separate a single, desired protein from the mixture of thousands generated by a living cell, which remains biotechnology's natural protein factory of choice.

Bacteria use the enzyme, called subtilisin, as a sort of food processor: After producing it internally, they release the enzyme into the soil, where it uses a minuscule "blade" to chop up proteins into digestible pieces. Because it could damage the bacterium's interior, the blade has a protective sheath that only comes off once the enzyme has exited the cell.

"The enzyme and sheath are strongly attracted to each other. The enzyme's first act is to cut the sheath away," says NIST's Travis Gallagher. "The method takes advantage of their attraction in order to isolate the protein we want."

The team first creates many "sheathless" copies of the enzyme, which are modified to function only in the presence of a triggering molecule such as fluoride. The modified enzymes are bound to the surface of a strainer. Then the team uses engineered cells to generate mass quantities of a po
'/>"/>

Contact: Chad Boutin
boutin@nist.gov
301-975-4261
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Developer of advanced computing memory, father of biochemical engineering, and innovative engineering educators win highest engineering honors of 2009
2. UT Southwestern researchers disrupt biochemical system involved in cancer, degenerative disease
3. Margarita Saenz, M.D. is recipient of Genzyme/ACMGF Fellowship in Biochemical Genetics
4. Kelvin Lee winner of Biochemical Engineering Journal Young Investigator Award
5. Newly created cancer stem cells could aid breast cancer research
6. Obesity and lack of exercise could enhance the risk of pancreatic cancer
7. Finding that 1-in-a-billion that could lead to disease
8. 60 second test could help early diagnosis of common brain diseases
9. Auto immune response creates barrier to fertility; could be a step in speciation
10. Paracetamol, one of most used analgesics, could slow down bone growth
11. Drug could improve pregnancy outcomes in wider range of women with insulin resistance
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Biochemical 'on-switch' could solve protein purification challenge
(Date:11/5/2014)... -Individuals show great diversity in their ability to identify ... greatly differ in their perceptual evaluation of odors, with ... , Sex differences in olfactory detection may ... be connected to one,s perception of smell, which is ... olfactory superiority has been suggested to be cognitive or ...
(Date:11/4/2014)... (November 4, 2014) — Think about the way our ... do neighboring cells know that they are supposed to ... do these tissues find the correct place and alignment? ... these crucial questions. , In a new study, UM ... communicate with their surrounding neighbors, at the head-trunk region. ...
(Date:11/4/2014)...   Neurotechnology , a provider of high-precision ... version of its fingerprint matching algorithm has achieved ... evaluation organized by NIST. The MINEX test measures ... 378 fingerprint standard template format. MINEX compliance is ... the United States and ...
Breaking Biology News(10 mins):The female nose always knows: Do women have more olfactory neurons? 2The inside story: How the brain and skull stay together 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 2Neurotechnology Places Second in Ongoing MINEX Ranking for Fingerprint Matching Algorithms 3
... WEST LAFAYETTE, Ind. - Honeybee populations have been in ... have identified one of the factors that cause bee ... dead in and around hives from several apiaries over ... insecticides, which are commonly used to coat corn and ...
... University of Illinois researchers have shown that by ... of metal nanoantennas, these nano-scale structures allow for ... particles. "Nanoantennas are extremely popular right ... optical fields in small areas," explained Kimani Toussaint, ...
... Calif. Scientists have long seen evidence of social behavior among ... the sea. Dolphins frolic together, lions live in packs, and ... the insects. And, right under our feet, it appears that ... the soil. Until recently, it was unknown how the worms ...
Cached Biology News:Researchers: Honeybee deaths linked to seed insecticide exposure 2Researchers: Honeybee deaths linked to seed insecticide exposure 3Optical nanoantennas enable efficient multipurpose particle manipulation 2Worm seeks worm: Caltech researchers find chemical cues driving aggregation in nematodes 2Worm seeks worm: Caltech researchers find chemical cues driving aggregation in nematodes 3
(Date:11/21/2014)... The California Healthcare Institute ... representing leading California academic institutions, biotechnology, medical device, ... today released a report revealing that California ... The trend shows a 4 percent increase between ... Industry Report indicates that, while biopharmaceutical and ...
(Date:11/21/2014)... (PRWEB) November 21, 2014 2014 ... Ferrocyanide Industry” is a professional and in-depth research ... Sodium ferrocyanide information, like its definition, classification, ... industry overview. This report covers the international market ... global (such as the US, Europe, Asia, China, ...
(Date:11/21/2014)... ON , Nov. 20, 2014 /PRNewswire/ - BIOREM Inc. (TSXV: BRM) ... and nine month periods ended September 30, 2014.  Biorem,s complete 2014 ... ( www.sedar.com ). Financial Summary:Three-months ended September ... except per share data) , 2014 , ... 2,282 , 5,281 , 6,715 ...
(Date:11/18/2014)... Diego, CA (PRWEB) November 17, 2014 Athena ... of Salk , Dr. Beverly Emerson, a professor in the ... of genes. Her most recent discovery points to an “off ... Dr. Emerson’s career and how her local research is making ... will present her findings and then join host Cheryl K. ...
Breaking Biology Technology:California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 2California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 3California Healthcare Institute and PwC Report Boom in California’s Biomedical Industry 4Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 2Sodium Ferrocyanide Market: Analysis for Global & China Regions Development Trends Available at DeepResearchReports.com 3Biorem Reports Third Quarter Results 2Biorem Reports Third Quarter Results 3
... ... Executive Officer of Merrimack Pharmaceuticals, will provide a company overview at the Jefferies 2010 ... ... announced that it will present at the Jefferies 2010 Global Life Sciences Conference in ...
... A team of Duke University chemists has perfected a simple ... conductors are small enough to be transparent, making them ideal ... displays. "Imagine a foldable iPad," said Benjamin Wiley, an ... findings online this week in Advanced Materials . ...
... CHAPEL HILL, N.C. , June 1 Companies invest hundreds of ... these new products to market.   , , ... on these vast investments, successful companies must maximize product uptake across all customer groups: ... win formulary access and improve product placement.   , ...
Cached Biology Technology:Merrimack Pharmaceuticals to Present at the Jefferies 2010 Global Life Sciences Conference 2Copper nanowires enable bendable displays and solar cells 2Maximize Market Research to Improve Formulary Access Within Managed Markets Sector 2Maximize Market Research to Improve Formulary Access Within Managed Markets Sector 3
...
... high-density serum biomarker chip to profile the ... antibody chip containing 120 human cancer serum ... biomarker discovery scientists to study panels of ... therapy and the likelihood of recurrence of ...
... Ventilates and removes chemical fumes, ... exhaust. Bonded charcoal filters remove ... ensure safe exhaust flow speed ... access opening. Includes air speed ...
Sigmas E-TOXATE (Limulus Amebocyte Lysate) test kits are intended for the detection and semiquantitation,of endotoxins for research purposes....
Biology Products: