Navigation Links
Biochemical 'on-switch' could solve protein purification challenge
Date:10/22/2009

Drugs based on engineered proteins represent a new frontier for pharmaceutical makers. Even after they discover a protein that may form the basis of the next wonder drug, however, they have to confront a long-standing problem: how to produce large quantities of the protein in a highly pure state. Now, a multi-institutional research team including a biochemist at the National Institute of Standards and Technology (NIST) may have found* a new solution in an enzymatic "food processor" they can activate at will.

The team has found an efficient method of harvesting purified protein molecules by altering an enzyme that soil bacteria use to break down their food. In its natural form, this enzyme would be of little use to drug developers, but the team has modified it so that it can be activated at the desired moment. By creating essentially an "on-switch" for the enzyme's activity, the team has found a way to separate a single, desired protein from the mixture of thousands generated by a living cell, which remains biotechnology's natural protein factory of choice.

Bacteria use the enzyme, called subtilisin, as a sort of food processor: After producing it internally, they release the enzyme into the soil, where it uses a minuscule "blade" to chop up proteins into digestible pieces. Because it could damage the bacterium's interior, the blade has a protective sheath that only comes off once the enzyme has exited the cell.

"The enzyme and sheath are strongly attracted to each other. The enzyme's first act is to cut the sheath away," says NIST's Travis Gallagher. "The method takes advantage of their attraction in order to isolate the protein we want."

The team first creates many "sheathless" copies of the enzyme, which are modified to function only in the presence of a triggering molecule such as fluoride. The modified enzymes are bound to the surface of a strainer. Then the team uses engineered cells to generate mass quantities of a po
'/>"/>

Contact: Chad Boutin
boutin@nist.gov
301-975-4261
National Institute of Standards and Technology (NIST)
Source:Eurekalert  

Page: 1 2

Related biology news :

1. Developer of advanced computing memory, father of biochemical engineering, and innovative engineering educators win highest engineering honors of 2009
2. UT Southwestern researchers disrupt biochemical system involved in cancer, degenerative disease
3. Margarita Saenz, M.D. is recipient of Genzyme/ACMGF Fellowship in Biochemical Genetics
4. Kelvin Lee winner of Biochemical Engineering Journal Young Investigator Award
5. Newly created cancer stem cells could aid breast cancer research
6. Obesity and lack of exercise could enhance the risk of pancreatic cancer
7. Finding that 1-in-a-billion that could lead to disease
8. 60 second test could help early diagnosis of common brain diseases
9. Auto immune response creates barrier to fertility; could be a step in speciation
10. Paracetamol, one of most used analgesics, could slow down bone growth
11. Drug could improve pregnancy outcomes in wider range of women with insulin resistance
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Biochemical 'on-switch' could solve protein purification challenge
(Date:8/21/2014)... study of American singles found that during sex ... orgasm rates. On average, men experience orgasm 85.1 ... making little difference. For women, however, orgasm occurrence ... 62.9 percent of the time during sex with ... with women,s sexual orientation, with lesbian women experiencing ...
(Date:8/21/2014)... to ignore, but they are the ultimate source of ... are increasingly dependent on algae, too, to suck up ... to the bottom of the ocean. Now, by using ... have evidence showing that viruses infecting those algae are ... when all else stays essentially the same, and this ...
(Date:8/21/2014)... landmark study aimed at improving the food intake, health ... care homes is among three projects at the University ... from the Canadian Institutes of Health Research (CIHR). ... Health Sciences at Waterloo, and a Schlegel research chair ... years to investigate why many Canadians living in long-term ...
Breaking Biology News(10 mins):Orgasm rates for single women less predictable than men's, vary by sexual orientation 2Orgasm rates for single women less predictable than men's, vary by sexual orientation 3Orgasm rates for single women less predictable than men's, vary by sexual orientation 4Viruses take down massive algal blooms, with big implications for climate 2Grants will fund landmark aging research at Waterloo 2
... Cleveland -- The use of padded headgear and gloves ... the head, according to newly published research from Cleveland ... Clinic,s Lutheran Hospital, the researchers replicated hook punches to ... pendulum. The impacts were measured under five padding configurations: ...
... This release is available in German . ... a draft version of the genome from a small fragment ... southern Siberia. The DNA sequences showed that this individual came ... become known as Denisovans. Together with their sister group the ...
... are you really eating when you,re eating chicken? Do different ... twins have similar fingerprints? How does sleep affect your memory? ... from 33 local schools will answer these and other intriguing ... the University of California, Riverside, Feb. 7-9. The fair will ...
Cached Biology News:Padded headgear, boxing gloves may offer some protection for fighters 2Entire genome of extinct human decoded from fossil 2Fair to bring future scientists and engineers to UC Riverside 2
(Date:8/20/2014)... Grade school history lessons often have it that American ... whooping cough, chicken pox, influenza and tuberculosis brought to ... while estimates vary, about 20 million people lived in ... percent of them were killed by European diseases., But ... Arizona State University and Johannes Krause of the University ...
(Date:8/20/2014)... , Aug. 20, 2014  Decision Resources Group finds that the ... Russia , India and ... in 2013, roughly equal to the size of the ... BRIC market will experience much faster growth as a result of ... Other key findings from Decision Resources Group,s coverage of ...
(Date:8/20/2014)... 20, 2014 /PRNewswire-iReach/ -- A case study from ... the school,s bioengineering department and the Intel® Internet ... Software Academic Program, UCSD,s research focuses on exploring ... body.  Photo - http://photos.prnewswire.com/prnh/20140819/137540 ... work of Dr. Todd P. Coleman , ...
(Date:8/19/2014)... One of the major limitations ... inadequate vascular supply. Nutrient and gas transport throughout ... critical for successful regeneration and integration into the ... in vascularization and promote angiogenesis in a variety ... design and architecture, and enhancing scaffolds with stem ...
Breaking Biology Technology:Unusual discovery leads to fascinating tuberculosis theory 2Unusual discovery leads to fascinating tuberculosis theory 3BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 2BRIC Vascular Access Device Market Valued at $1.6 Billion in 2013, Similar to the U.S. Market 3Intel and University of San Diego Bioengineering Department Release Case Study on Health Sciences Research 2Vascularization in Tissue Engineering Highlighted at 12th New Jersey Symposium on Biomaterials Science 2
... to countless,complaints from conventional lubricant users about being messy ... which has proven popular in the United States, is ... that took women,s ideas about what,a lubricant should be ... Lubricant Mousse has all the benefits of a,lubricant without ...
... for clean water stretches even the resources of the world,s ... technology and novel ideas to find solutions. , Mark Shannon ... of world leaders in water resource research to address this ... issue of Nature. , "As dire as the growing problems ...
... MANASQUAN, N.J., March 21 BioSpace, the world,s leading,online ... will host,a Life Science Career Fair in King of ... take place at the Radisson Valley Forge from,2pm to ... science professionals from across Pennsylvania, New York, New,Jersey and ...
Cached Biology Technology:Seduce with Mousse - Replens Introduces Intimate Options: First Personal Lubricant Mousse in Canada 2Countering an approaching water crisis 2BioSpace to Host Life Science Career Fair in Pennsylvania 2
Anti-Mouse C-10, Biotin (Polyclonal) (rabbit IgG)...
... ADAMTS16 ( Abpromise for all ... Synthetic peptide based on the ... the amino acid sequence is proprietary) ... 170690 SWISS Protein ...
Purified Mouse anti-Hu Actopaxin (pS8) Storage Temperature: Refrigerate(2 to 8C)...
Hamartin/TSC1 (1B2) Mouse mAb Ship: Hot Store: -20 C...
Biology Products: