Navigation Links
Better tissue healing with disappearing hydrogels
Date:6/6/2014

When stem cells are used to regenerate bone tissue, many wind up migrating away from the repair site, which disrupts the healing process. But a technique employed by a University of Rochester research team keeps the stem cells in place, resulting in faster and better tissue regeneration. The key, as explained in a paper published in Acta Biomaterialia, is encasing the stem cells in polymers that attract water and disappear when their work is done.

The technique is similar to what has already been used to repair other types of tissue, including cartilage, but had never been tried on bone.

"Our success opens the door for manyand more complicatedtypes of bone repair," said Assistant Professor of Biomedical Engineering Danielle Benoit. "For example, we should now be able to pinpoint repairs within the periosteumor outer membrane of bone material."

The polymers used by Benoit and her teams are called hydrogels because they hold water, which is necessary to keep the stem cells alive. The hydrogels, which mimic the natural tissues of the body, are specially designed to have an additional feature that's vital to the repair process; they degrade and disappear before the body interprets them as foreign bodies and begins a defense response that could compromise the healing process.

Because stem cells have the unique ability to develop into many different types of cells, they are an important part of the mechanism for repairing body tissue. At present, unadulterated therapeutic stem cells are injected into the bone tissue that needs to be repaired. Benoit believed hydrogels would allow the stem cells to finish the job of initiating repairs, then leave before overstaying their welcome.

The research team tested the hypothesis by transplanting cells onto the surface of mouse bone grafts and studying the cell behavior both in vivoinside the animaland in vitrooutside the body. They started by removing all living cells from donor bone fragments, so that the tissue regeneration could be accomplished only by the stem cells.

In order to track the progress of the research, the stem cells were genetically modified to include genes that give off fluorescence signals. The bone material was then coated with the hydrogels, which contained the fluorescently labeled stem cells, and implanted into the defect of the damaged mouse bone. At that point, the researchers began monitoring the repair process with longitudinal fluorescence to determine if there would be an appreciable loss of stem cells in the in vivo samples, as compared to the static, in vitro, environments. They found that there was no measurable difference between the concentrations of stem cells in the various samples, despite the fact that the in vivo sample was part of a dynamic environmentwhich included enzymes and blood flowmaking it easier for the stem cells to migrate away from the target site. That means virtually all the stem cells stayed in place to complete their work in generating new bone tissue.

"Some types of tissue repair take more time to heal than do others," said Benoit. "What we needed was a way to control how long the hydrogels remained at the site."

Benoit and her team were able to manipulate the time it took for hydrogels to dissolve by modifying groups of atomscalled degradable groupswithin the polymer molecules. By introducing different degradable groups to the polymer chains, the researchers were able to alter how long it took for the hydrogels to degrade.

Benoit believes degradable hydrogels show promise in many research areas. For example, it may be possible to initiate tissue regeneration after heart attacks without having a patient undergo difficult, invasive surgery, but a great deal of additional research is required.


'/>"/>

Contact: Peter Iglinski
peter.iglinski@rochester.edu
585-273-4726
University of Rochester
Source:Eurekalert  

Related biology news :

1. Toward a better drug against malaria
2. New research provides better understanding of endometriosis
3. Building a better blood vessel
4. Engineering a better way to rebuild bone inside the body
5. Imaging scientists develop a better tool for tracking MS
6. New insights into premature ejaculation could lead to better diagnosis and treatment
7. Better science for better fisheries management
8. Making better medicines with a handful of chemical building blocks
9. Silly Putty material inspires better batteries
10. Understanding the 1918 flu pandemic can aid in better infectious disease response
11. Green-energy community projects need better government backing
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Better tissue healing with disappearing hydrogels
(Date:6/22/2016)...  The American College of Medical Genetics and Genomics was ... as one of the fastest-growing trade shows during the Fastest ... in Las Vegas . ... in each of the following categories: net square feet of ... attendees. The 2015 ACMG Annual Meeting was ranked 23 out ...
(Date:6/22/2016)... 22, 2016 On Monday, the Department of ... to share solutions for the Biometric Exit Program. The ... Border Protection (CBP), explains that CBP intends to add ... the United States , in order to ... imposters. Logo - http://photos.prnewswire.com/prnh/20160622/382209LOGO ...
(Date:6/20/2016)... , June 20, 2016 Securus Technologies, ... technology solutions for public safety, investigation, corrections and ... prisons involved, it has secured the final acceptance ... facilities for Managed Access Systems (MAS) installed. Furthermore, ... facilities to be installed by October, 2016. MAS ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 2016 , ... Mosio, a leader in clinical research patient ... and Retention Tips.” Partnering with experienced clinical research professionals, Mosio revisits the hurdle ... and strategies for clinical researchers. , “The landscape of how patients receive and ...
(Date:6/23/2016)... , June 23, 2016 Houston ... with the Cy-Fair Sports Association to serve as ... the agreement, Houston Methodist Willowbrook will provide sponsorship ... and connectivity with association coaches, volunteers, athletes and ... with the Cy-Fair Sports Association and to bring ...
(Date:6/23/2016)... ... June 23, 2016 , ... Supplyframe, the Industry Network ... Supplyframe Design Lab . Located in Pasadena, Calif., the Design Lab’s mission is ... projects are designed, built and brought to market. , The Design Lab is ...
(Date:6/23/2016)... 23, 2016 Andrew ... http://doi.org/10.17925/OHR.2016.12.01.22 Published recently in ... journal from touchONCOLOGY, Andrew D Zelenetz , ... cancer care is placing an increasing burden on ... biologic therapies. With the patents on many biologics ...
Breaking Biology Technology: