Navigation Links
Berkeley Lab researchers get a detailed look at a DNA repair protein in action
Date:10/23/2013

ciences Division.

Hura is the lead author of a paper describing this research in the Proceedings of the National Academy of Sciences (PNAS). The article is titled "DNA conformations in mismatch repair probed in solution by X-ray scattering from gold nanocrystals." The corresponding author is John Tainer, who holds joint appointments with Berkeley Lab's Life Sciences Division and the Scripps Research Institute. (See below for complete list of co-authors)

"It is a common belief that DNA is a passive component in protein interactions that involve DNA metabolism, but many proteins actually make use of DNA structural features, such as rigidity and conformation for important biological processes," Tainer says. "The view of DNA as a passive element is at least in part due to a paucity of robust tools for examining dynamic DNA conformational states during multistep reactions."

For this study, Tainer, Hura and their colleagues were able to capitalize on the high quality X-ray beams at ALS beamline 12.3.1, also known as SIBYLS, which stands for Structurally Integrated Biology for Life Sciences. Maintained by Berkeley Lab's Life Sciences Division under the direction of Tainer, the SIBYLS experimental station is optimized for SAXS imaging, which provides global information on the conformations adopted by a population of macromolecules in almost any solution condition.

"Because X-rays scatter predominantly from electrons, the use of gold nanocrystals provides extremely high contrast relative to organic molecules critical for biology," Tainer says. "This is important because we can follow specific biological molecules in complex reactions and along pathways to understand how their changes in shape and assembly control the biological outcomes."

The SAXS study at the SIBYLS beamline validated what has been dubbed the "beads-on-a-string" model of DNA repair, in which MutS proteins are the beads and DNA is the string. In solution
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Berkeley Lab scientists generate electricity from viruses
2. Berkeley Lab scientists help define the healthy human microbiome
3. Waves of Berkeley Lab responders deploy omics to track Deepwater Horizon cleanup microbes
4. UC Berkeley chemists installing first carbon dioxide sensor network in Oakland
5. UC Berkeley survey shows college campuses can make good bird havens
6. Berkeley Lab scientists help develop promising therapy for Huntingtons disease
7. UC Berkeley, UCSF and Stanford join forces to help commercialize university innovations
8. Study by UC Santa Barbara researchers suggests that bacteria communicate by touch
9. UC Santa Barbara researchers discover genetic link between visual pathways of hydras and humans
10. Researchers attempt to solve problems of antibiotic resistance and bee deaths in one
11. UNH researchers find African farmers need better climate change data to improve farming practices
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Berkeley Lab researchers get a detailed look at a DNA repair protein in action
(Date:4/17/2014)... Our eyes not ... with a continuous stream of information about our own movements. ... a car the world glides by us and leaves ... our brain calculates self-motion from this "optic flow". This way, ... during our own movements. Together with biologists from the University ...
(Date:4/17/2014)... to produce hydrogen sulfide in order to properly multiply ... from the Center for Craniofacial Molecular Biology at the ... Songtao Shi, principal investigator on the project, said the ... the flow of calcium ions. The essential ions activate ... or the creation of new bone tissue, and keeps ...
(Date:4/17/2014)... Singh Thursday as a "Champion of Change" for ... in the residential, commercial and industrial sectors. , ... and Computer Engineering and director of Clemson,s Center ... leading the charge across the country to create ... driving policy changes at the local level to ...
Breaking Biology News(10 mins):How vision makes sure that little fish do not get carried away 2How vision makes sure that little fish do not get carried away 3How vision makes sure that little fish do not get carried away 4White House honors Clemson professor as 'Champion of Change' for solar deployment 2
... international team of scientists led by King,s College London has ... (short-sightedness). Myopia is a major cause of ... cure. These findings, published today in the journal Nature ... lead to finding better treatments or ways of preventing the ...
... (NGS) technology has revolutionized biomedical research, making complete ... for a wide variety of research applications. Bioinformatics ... critical bottleneck for many researchers and organizations wishing ... DNA Sequencing Informatics provides a thorough introduction ...
... throughout Vienna it was impossible not to notice that the ... around the courtyards of the inner city nor woke tired ... no longer there. Thankfully, they gradually reappeared and a ... levels. But the sudden crash in numbers was alarming ...
Cached Biology News:New genes for short-sightedness identified 2Forensic pathology: tracing the origin of the Usutu Virus 2
(Date:1/14/2014)... WASHINGTON , Jan. 14, 2014  3D Communications, a leading provider ... scientific, regulatory, business, and media events in the United ... its former associate Virginia Cox , JD, is returning to ... Virginia Cox re-joins 3D after more than two years ...
(Date:1/14/2014)... , Jan. 14, 2014 The largest international ... medicinal plants and therapeutic derivatives thereof has endorsed ... and researchers about the challenges of adulterated herb ... ) The Society for Medicinal ...
(Date:1/14/2014)... Canada (PRWEB) January 14, 2014 In ... its motives and methods in product development and promotion ... the industry. This mistrust, fueled by concerns about the ... fed by reports of spectacular fines to the world’s ...
(Date:1/14/2014)... ®, provider of RapidResponse ®, a cloud-based supply chain ... S&OP ) service, is proud to be a gold sponsor ... at the Hilton San Diego Bayfront, January 30 – 31, ... , Director of Supply Chain at Amgen, as she presents ...
Breaking Biology Technology:Former FDA Associate Commissioner Returns To 3D Communications 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 2World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 3World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 4World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 5World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 6World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 7World's Largest Group of Medicinal Plant Researchers Endorses ABC-AHP-NCNPR Botanical Adulterants Program 8The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2
... New sol-gel inks developed by researchers at the University ... three-dimensional structures of metal oxides with nanoscale features. , ... nanoscale opens a new avenue to functional devices. Potential ... The researchers describe the new inks in a paper ...
... Calif., Oct. 11 CompareNetworks,Media Group, the leading ... industries, today announced the launch of Acuity Medical,Education, ... CompareNetworks. With the arrival of two key new ... Liza Frenzik as Special,Projects Director, the CompareNetworks team ...
... Fisher,Scientific Inc. (NYSE: TMO ), the world ... construct an $11 million, state-of-the-art BioCenter,for the manufacture ... the site of its existing bioprocess production business ... a response to the increasing demand for,disposable bioprocessing ...
Cached Biology Technology:Sol-gel inks produce complex shapes with nanoscale features 2CompareNetworks Media Group Delivers CME/CE Through Acuity Medical Education 2Thermo Fisher Scientific to Expand Manufacturing Operation for Single-Use Bioprocessing Containers 2Thermo Fisher Scientific to Expand Manufacturing Operation for Single-Use Bioprocessing Containers 3Thermo Fisher Scientific to Expand Manufacturing Operation for Single-Use Bioprocessing Containers 4
iEMS Thermal Microplate Holder...
Improve inventory management with stainless steel red cell canisters; ensure product safety with CO2 or LN2 backup systems. A complete solution for your blood banking needs....
Improve inventory management with stainless steel red cell canisters; ensure product safety with CO2 or LN2 backup systems. A complete solution for your blood banking needs....
... RNase H- Reverse Transcriptase (RT) is an ... RT. It is a DNA polymerase that ... RNA DNA or an RNA:DNA hybrid. This ... of point mutations rather than a deletion ...
Biology Products: