Navigation Links
Basic guides to PCR, labeling neurons featured in Cold Spring Harbor Protocols
Date:4/1/2009

COLD SPRING HARBOR, N.Y. (Wednesday, April 1, 2009) Along with new cutting-edge methods, Cold Spring Harbor Protocols is home to an in-depth library of basic laboratory methods. The April issue (www.cshprotocols.org/TOCs/toc4_09.dtl) features two of these standard techniques.

From molecular biology researchers to law enforcement forensics laboratories, polymerase chain reaction (PCR) is the commonly used method for nucleic acid amplification. But PCR is often difficult to optimize, and failure to do so can lead to undefined and unwanted products, or a complete lack of amplification altogether. To help avoid these issues, Kenneth Roux from Florida State University (http://bio.fsu.edu/~roux/) provides "Optimization and Troubleshooting in PCR." The article addresses various optimization strategies including touchdown PCR and hot-start PCR. Magnesium concentration, buffer pH, and cycling conditions are also considered. The article is freely accessible on the website for "Cold Spring Harbor Protocols" (http://cshprotocols.cshlp.org/cgi/content/full/2009/4/pdb.ip66).

The loading of individual cells with fluorescent probes via patch pipettes is regularly performed in neuroscience research laboratories. This method allows for combined electrophysiological and optical measurements at a quantitative level. Arthur Konnerth and colleagues from the Institut fr Physiologie der Ludwig-Maximilians-Universitt Mnchen (http://www.imprs-ls.de/professors/konnerth.htm) have written "Dye Loading with Patch Pipettes," available in the April issue. This patch-clamp methodology has been successful for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A wide range of dyes can be loaded using this method, including probes for morphological reconstruction, ion-sensitive indicator dyes for monitoring second-messenger cascades, and dye-labeled proteins for fluorescence resonance energy transfer (FRET), fluorescence correlation spectroscopy (FCS), and fluorescence recovery after photobleaching (FRAP) studies. The most widespread application of this technique has been for Ca2+ imaging. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/4/pdb.prot5201).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Getting down to cancer basics
2. Basic research critical to Americas economic recovery
3. NIST guides genetic genealogy labs toward improved accuracy
4. Polarized light guides cholera-carrying midges that contaminate water supplies
5. Scientists discover a molecular scaffold that guides connections between brain cells
6. U. of I. librarian launches comprehensive Web database of field guides
7. Odd protein interaction guides development of olfactory system
8. American College of Medical Genetics responds to new FDA labeling decision for warfarin
9. ORNL, St. Jude track neurons to predict and prevent disease
10. UCLA stem cells scientists make electrically active motor neurons from iPS cells
11. Researchers generate functional neurons from somatic cells
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/27/2017)... CENTRE, N.Y. , March 27, 2017 /PRNewswire-USNewswire/ ... Healthcare Information and Management Systems Society (HIMSS) Analytics ... Outpatient EMR Adoption Model sm . In addition, ... 12% of U.S. hospitals using an electronic medical ... CHS for its high level of EMR usage ...
(Date:3/24/2017)... -- Research and Markets has announced the addition of ... - Industry Forecast to 2025" report to their offering. ... The Global Biometric Vehicle ... around 15.1% over the next decade to reach approximately $1,580 million ... estimates and forecasts for all the given segments on global as ...
(Date:3/23/2017)... Research and Markets has announced the addition of the "Global ... 2025" report to their offering. ... The Global Vehicle Anti-Theft System Market is ... next decade to reach approximately $14.21 billion by 2025. ... all the given segments on global as well as regional levels ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... BioMedGPS announces expanded coverage of ... newest module, US Hemostats & Sealants. , SmartTRAK’s US Market for Hemostats and ... synthetic sealants and biologic sealants used in surgical applications. BioMedGPS estimates the market ...
(Date:10/11/2017)... ... October 11, 2017 , ... The CRISPR-Cas9 system ... experiments and avoiding the use of exogenous expression plasmids. The simplicity of programming ... systematic gain-of-function studies. , This complement to loss-of-function studies, such as with ...
(Date:10/11/2017)... , Oct. 11, 2017  VMS BioMarketing, a leading provider ... nationwide oncology Clinical Nurse Educator (CNE) network, which will launch ... for communication among health care professionals to enhance the patient ... office staff, and other health care professionals to help women ... cancer. ...
(Date:10/11/2017)... ... October 11, 2017 , ... Singh Biotechnology today announced ... to SBT-100, its novel anti-STAT3 (Signal Transducer and Activator of Transcription 3) B ... to cross the cell membrane and bind intracellular STAT3 and inhibit its function. ...
Breaking Biology Technology: