Navigation Links
Basic guides to PCR, labeling neurons featured in Cold Spring Harbor Protocols
Date:4/1/2009

COLD SPRING HARBOR, N.Y. (Wednesday, April 1, 2009) Along with new cutting-edge methods, Cold Spring Harbor Protocols is home to an in-depth library of basic laboratory methods. The April issue (www.cshprotocols.org/TOCs/toc4_09.dtl) features two of these standard techniques.

From molecular biology researchers to law enforcement forensics laboratories, polymerase chain reaction (PCR) is the commonly used method for nucleic acid amplification. But PCR is often difficult to optimize, and failure to do so can lead to undefined and unwanted products, or a complete lack of amplification altogether. To help avoid these issues, Kenneth Roux from Florida State University (http://bio.fsu.edu/~roux/) provides "Optimization and Troubleshooting in PCR." The article addresses various optimization strategies including touchdown PCR and hot-start PCR. Magnesium concentration, buffer pH, and cycling conditions are also considered. The article is freely accessible on the website for "Cold Spring Harbor Protocols" (http://cshprotocols.cshlp.org/cgi/content/full/2009/4/pdb.ip66).

The loading of individual cells with fluorescent probes via patch pipettes is regularly performed in neuroscience research laboratories. This method allows for combined electrophysiological and optical measurements at a quantitative level. Arthur Konnerth and colleagues from the Institut fr Physiologie der Ludwig-Maximilians-Universitt Mnchen (http://www.imprs-ls.de/professors/konnerth.htm) have written "Dye Loading with Patch Pipettes," available in the April issue. This patch-clamp methodology has been successful for single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations. A wide range of dyes can be loaded using this method, including probes for morphological reconstruction, ion-sensitive indicator dyes for monitoring second-messenger cascades, and dye-labeled proteins for fluorescence resonance energy transfer (FRET), fluorescence correlation spectroscopy (FCS), and fluorescence recovery after photobleaching (FRAP) studies. The most widespread application of this technique has been for Ca2+ imaging. This method is freely accessible on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/4/pdb.prot5201).


'/>"/>

Contact: David Crotty
crotty@cshl.edu
516-422-4007
Cold Spring Harbor Laboratory
Source:Eurekalert

Related biology news :

1. Getting down to cancer basics
2. Basic research critical to Americas economic recovery
3. NIST guides genetic genealogy labs toward improved accuracy
4. Polarized light guides cholera-carrying midges that contaminate water supplies
5. Scientists discover a molecular scaffold that guides connections between brain cells
6. U. of I. librarian launches comprehensive Web database of field guides
7. Odd protein interaction guides development of olfactory system
8. American College of Medical Genetics responds to new FDA labeling decision for warfarin
9. ORNL, St. Jude track neurons to predict and prevent disease
10. UCLA stem cells scientists make electrically active motor neurons from iPS cells
11. Researchers generate functional neurons from somatic cells
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/14/2017)... 2017  Wake Forest Baptist Medical Center today announced Julie ... executive officer (CEO). Freischlag joins the medical center on ... , M.D., who last year announced that he would ... after leading it since 2008.   As ... Forest Baptist,s academic health system, which includes Wake Forest ...
(Date:2/13/2017)... 13, 2017 Former 9/11 Commission border counsel ... Janice Kephart of Identity Strategy Partners, LLP, ... Trump,s "Executive Order: Protecting the Nation From Foreign ... "As President Trump,s ,Travel Ban, Executive Order ... essentially banned the travel ban, it is important that ...
(Date:2/9/2017)... , Feb. 9, 2017 The biomass boiler ... of the biomass boiler market globally in terms of ... biomass boilers. The market for biomass boilers has been ... end-user, application, and country/region. The market based on feedstock ... forest residues, biogas & energy crops, urban residues, and ...
Breaking Biology News(10 mins):
(Date:3/29/2017)... March 29, 2017 QIAGEN N.V. (NASDAQ: QGEN ... the U.S. launch of its ipsogen ® JAK2 RGQ PCR ... and Drug Administration as a qualitative in vitro diagnostic test for the detection ... ... processed on QIAGEN,s Rotor-Gene ® Q MDx system, which is a member ...
(Date:3/29/2017)... ... March 29, 2017 , ... (March 29, 2017) ... has been approved as an active member of the Mexican Direct Selling ... distributers and consumers in relationship marketing. This professional organization fosters loyal and fair ...
(Date:3/29/2017)... ... 29, 2017 , ... ACEA Biosciences, a privately owned biotechnology ... award to noteworthy scientists who will be presenting research using ACEA Biosciences’ technology ... awards are being given to two postdoctoral fellows studying pathogen capture by neutrophils, ...
(Date:3/29/2017)... , March 29, 2017 /PRNewswire/ - The University ... partners Nordion, a business of Sterigenics International, and General ... has been submitted to the U.S. Nuclear Regulatory Commission ... U.S. production of molybdenum-99 (Mo-99). Once operational, production from ... of U.S. demand for Mo-99, which currently must be ...
Breaking Biology Technology: