Navigation Links
Bacterial reporters that get the scoop
Date:3/17/2014

ling dogma in synthetic biology, which is to design genetic systems that drive cell behavior from scratch, said Wyss Institute Senior Staff Scientist Jeff Way, Ph.D., a coauthor on the paper. On the other hand, "Nature has a tried-and-true blueprint for memory systems if you know where to look," Way said. "Why not just accept Nature as it is, and develop the system from there?"

The genetic switch the team inserted in E. coli came from lambda phage, a virus that commonly attacks this bacterium.

After invading E. coli, lambda typically lays low, living in a stealth mode called lysogeny in which its DNA simply hangs out in the E. coli's genome. But when the bacterium's DNA is damaged and only then the switch flips, instructing the virus to enter a mode called lysis in which it multiplies inside the cell and breaks through its membrane in a kind of microbial explosion.

"This is a very stable system in Nature," said lead author Jonathan Kotula, Ph.D., a Postdoctoral Fellow at HMS who is also affiliated with the Wyss Institute. "We knew the lambda switch would be a great candidate for the memory element, and we simply tweaked it to meet our needs."

The cells with the engineered lambda switch would not become lytic under any conditions. Kotula and the rest of Silver's team used standard molecular genetic tools to rig the switch such that it turned on only in the presence of an inactive form of the antibiotic tetracycline.

In laboratory experiments, the switch turned on within a few hours of exposure to the antibiotic and stayed in this 'ON' state inside E. coli for a week or more, even as the bacteria grew and divided. In short, the cells "remembered" that they had seen that molecule in the gut.

"It was truly shocking how cleanly the experiments worked," said Jordan Kerns, Ph.D., a Wyss Institute Postdoctoral Fellow.

But to function as a living diagnostic, the engineered
'/>"/>

Contact: Kristen Kusek
kristen.kusek@wyss.harvard.edu
617-432-8266
Wyss Institute for Biologically Inspired Engineering at Harvard
Source:Eurekalert  

Page: 1 2 3 4

Related biology news :

1. Spread of antibiotic resistance understood by unravelling bacterial secretion system
2. Bacterial fibers critical to human and avian infection
3. £4 million to tackle spread of bacterial infections
4. Puzzling question in bacterial immune system answered
5. Little but lethal -- small RNAs coordinate bacterial attack on epithelial cells
6. Gut microbes affect MicroRNA response to bacterial infection
7. In surprise finding, blood clots absorb bacterial toxin
8. New method of DNA editing allows synthetic biologists to unlock secrets of a bacterial genome
9. Structure of bacterial nanowire protein hints at secrets of conduction
10. Evidence of 3.5 billion-year-old bacterial ecosystems found in Australia
11. Changing the conversation -- polymers disrupt bacterial communication
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
Bacterial reporters that get the scoop
(Date:10/15/2014)... a non-traditional biophysics textbook and it describes how life ... of discovery into biological systems and provides an understanding ... is about how our genes make proteins that control ... cells in an organism. It quantifies the principles of ... be found on both large and small scales. The ...
(Date:10/15/2014)... Fred Alt will be awarded the 44th Rosenstiel Award ... for his pioneering research exploring the mechanisms of genomic ... cancer cells. Alt is the second alumnus to win ... Nobel Prize in 2003. , Alt is the Charles ... at Harvard Medical School and an investigator at the ...
(Date:10/14/2014)... have sequenced the house fly genome for the first ... from an insect that thrives in pathogen-rich dung piles ... in the journal Genome Biology , will increase ... how flies quickly adapt to resist insecticides, which could ... (Musca domestica) carry and transmit more than 100 human ...
Breaking Biology News(10 mins):New book about life as seen from physics 2Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2House fly genome reveals expanded immune system 2
... of American Societies for Experimental Biology (FASEB) announces ... Conference (SRC): Matricellular Proteins in Development, Health, and ... extracellular matrix (ECM) are the basis for multi-cellular ... in all metazoan organisms. Structural ECM components such ...
... Societies for Experimental Biology (FASEB) announces the opening ... Mechanisms in Plant Development Conference. The 2013 ... and unique scientific conference where the intersections of ... through formal talks, poster sessions, and intensive scientific ...
... Two wasp species , Calymmochilus dispar and ... on ant-eating spiders in a study published in the ... spider, Zodarion styliferum , belongs to the largest genus ... and North Africa, significantly with at least 35 species reported ...
Cached Biology News:FASEB SRC announces conference: Matricellular Proteins in Development, Health, and Disease 2Survival of the fittest: Predator wasps breed at the expense of spider juveniles 2
(Date:10/19/2014)... NextCODE Health, which enables clinicians and researchers to ... today announced the launch of its new genomic data analysis ... American Society of Human Genetics (ASHG) annual meeting in ... and apply for free beta access, visit www.nextcode.com . ... be viewed here . The Exchange: ...
(Date:10/19/2014)... October 19, 2014 The report "Chocolate, ... Share, Trade, Prices, Geography Trend and Forecast (2011 - ... category, and geography and studies the major market drivers, ... America, Europe, and Asia. , The global chocolate market ... $98.3 billion in 2016 at an estimated CAGR of ...
(Date:10/18/2014)... October 19, 2014 The “Human ... Acting, Intermediate Acting, Premixed), Modern Human Insulin (Rapid ... Levemir, NovoRapid, Novomix, Tresiba, Others) - Forecast to ... drivers, restraints, challenges, opportunities, current market trends, and ... with the estimates and forecasts of the revenue ...
(Date:10/18/2014)... The Asia-Pacific hardware encryption display market report defines and ... forecast of revenue. This market was valued $5,154.0 million ... by 2018, at a CAGR of 58.4% from 2013 ... Asia-Pacific hardware encryption market report, to get an idea ... glimpse of the segmentation of the market, and is ...
Breaking Biology Technology:The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 2The NextCODE Exchange: The first global, real-time system for sharing full-resolution genomic data 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 2Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 3Chocolate Market Projected To Reach $98.3 Billion by 2016 - New Report by MarketsandMarkets 4Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 2Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 3Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 4Medical Human Insulin Market Is Expected to Reach $42 billion by 2019 - New Research Report by MarketsandMarkets 5The Asia-Pacific hardware encryption display market is expected to reach $51,362.6 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific hardware encryption display market is expected to reach $51,362.6 million by 2018 - New Report by MicroMarket Monitor 3
... busy at the BIO Chicago industry show this week. Rather ... pen or kangaroo lapel pin, he met with more than ... mostly in prearranged sessions designed to help attendees make the ... of NeoClone , which manufactures monoclonal antibodies. It was ...
... some prominent customers led to a new high in ... of 2006, the company reported Wednesday. , ,Specific figures ... quarter by exceeding expectations in license sales, services business, ... established in the fourth quarter of 2005. The company ...
... representatives from Japan, the second-largest biotech market in ... BIO convention, you might not think they would ... a show of Wisconsin's international partnerships in biotechnology, ... at the Japan international session. And speaker Tsuney ...
Cached Biology Technology:Partner program is highlight of BIO for Wisconsin firm 2Japanese delegates highlight Wisconsin at BIO Chicago 2Japanese delegates highlight Wisconsin at BIO Chicago 3
... Vector NTI Advance 9.1 ... and highly integrated application ... and molecular biology data ... readily available for export. ...
... 50mM Tris-HCl, pH 7.5, 0.1mM ... 1mM benzamidine, 0.1% 2-mercaptoethanol and ... Phosphatase 2A1) partially purified enzyme ... QSepharose,amino-hexyl agarose, Superdex 200, and ...
Whole blood is collected from mice (Swiss Webster, 8-10 weeks old,either sex) and processed within 45 minutes of collection. The complement is then filtered and pooled on ice, bottled and stored at -...
... vitro cell labeling mix, 92.5 ... solution stabilized with 0.1% 2-mercaptoethanol ... 3,4-dicarboxylic acid. *Amino acid mixture ... containing l-[35S]Methionine and l-[35S]Cysteine. * ...
Biology Products: