Navigation Links
Backing out of the nanotunnel
Date:1/10/2012

increasingly being employed to investigate the secondary structures of RNA and DNA," Gerland points out. "Passage through narrow nanopores causes the sequence to unfold, and the dynamics of translocation provide insights into the structural features of the molecules, without the need to modify them by adding a fluorescent label. The technique is relatively new, and its potential has not yet been fully explored."

In the new study, he and his collaborators used a new experimental procedure, which allowed them to quantitatively describe the passage of simply structured polynucleotide sequences through nanopores, and develop a theoretical model that accounts for their findings. This level of understanding has not been achieved previously, because complicating factors such as interactions between the protein nanopore and the polynucleotide have had a significant influence on the measurements and made it difficult to predict the behavior of the test molecules.

Thanks to a clever change in experimental design, the impact of these factors has now been minimized. The trick is to perform the measurements on molecules as they translocate through the pore in reverse. First, the polynucleotide of interest is forced through the conical orifice from one side under the influence of an electrical potential. This causes its secondary structure to unfold and, as it emerges, the molecule refolds. An anchor at the end of the polynucleotide chain prevents it from passing completely through the pore onto the other side. For the return journey the potential is reversed, so that the process of unfolding now begins at the narrow end of the pore, and at this point the analysis is initiated.

"In contrast to the situation during forward translocation, no significant interactions appear to take place during the reverse trip," says Simmel.

On the basis of their experimental measurements, the researchers went on to construct a theoretical model that enabled
'/>"/>

Contact: Dr. Katrhin Bilgeri
kathrin.bilgeri@lmu.de
49-892-180-6938
Ludwig-Maximilians-Universitt Mnchen
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. No more free rides for piggy-backing viruses
2. Regional partnership to develop algal biofuels gets backing of San Diego leaders
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/17/2014)... full of wild animals and plant life, but there,s ... churning away in the soil, decomposing organic matter and ... role these fungi play in ecological systems, their identities ... scientists has generated a genetic map of more than ... was published this week in the Proceedings of ...
(Date:4/17/2014)... International researchers, led by the Australian National University ... sea-level changes and deep-sea temperature variability over the ... scientists better understand the climate surrounding ice ages ... help determine the relationship between carbon dioxide levels, ... ANU, the University of Southampton (UoS) and the ...
(Date:4/17/2014)... about domestication derive from Charles Darwin, whose ideas ... practices during the 19th century, a period when ... It is from Darwin that we inherit the ... from wild species and total human control over ... in this industrial setting has been applied too ...
Breaking Biology News(10 mins):Stanford biologists help solve fungal mysteries 2Stanford biologists help solve fungal mysteries 3Ancient sea-levels give new clues on ice ages 2The story of animal domestication retold 2The story of animal domestication retold 3The story of animal domestication retold 4
... the MUHC have discovered a gene that controls the ... a gene has been discovered for this disease. The ... Academy of Science (PNAS) this week provides a new ... and may contribute to public health efforts aimed at ...
... A major cause of hospital-acquired infections can persist ... found in healthcare settings, including bed linens, computer ... presented today at the 105th General Meeting of ... aureus (MRSA) is a major cause of hospital-acquired ...
... A major cause of hospital-acquired infections can persist ... found in healthcare settings, including bed linens, computer ... presented today at the 105th General Meeting of ... aureus (MRSA) is a major cause of hospital-acquired ...
Cached Biology News:Scientists discover gene that controls speed of tuberculosis development 2
(Date:1/14/2014)... (PRWEB) January 14, 2014 In recent ... motives and methods in product development and promotion has ... industry. This mistrust, fueled by concerns about the insidious ... by reports of spectacular fines to the world’s biggest ...
(Date:1/14/2014)... January 14, 2014 Global Record ... innovative information technology solutions for patients, physicians, the ... stakeholders, announced today the signing of a three-year ... and Drug Administration (FDA). This initiative is ...
(Date:1/14/2014)... Bellingham, Washington, USA, and Cardiff, UK (PRWEB) January 13, ... and photonics technology development leader with more than 20 ... international society for optics and photonics . Hainsey will ... “We are delighted to have Dr. Hainsey join SPIE ...
(Date:1/14/2014)... 2014 During the 1600’s through the 1800’s ... Doctor’s Plague.” In this time period, doctors did not know ... times, to the death of vulnerable patients. In the same ... they may be unwittingly transmitting herpes viruses to their patients. ...
Breaking Biology Technology:The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Global Record Systems Announces Research Collaboration Agreement with FDA to Create a Novel “Big Data” Paradigm for Collection of Patient Safety and Outcomes Information 2Photonics R&D Leader Bob Hainsey Joins SPIE Technical Staff 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 3
... Healthcare Quality has been chosen to lead an effort ... participation will not necessarily be based on the level of ... pilot project to develop a practical method of collecting performance ... that is useful to both physicians and consumers. While the ...
... keynote at the Fusion2006 CEO-CIO Symposium , WTN caught up ... and when it's a good idea. Read part one . ... of distributing the risk of development to other organizations. But doesn't ... , Koulopoulos Koulopoulos: Probably the second most ...
... more than 100 people have been infected with the H5N1 ... the fact that the virus does not spread easily from ... biomedical puzzle. , ,Now, a study of cells in the ... cells of the system that makes it difficult for the ...
Cached Biology Technology:Healthcare quality initiative shuns high-tech for now 2IT outsourcing according to Tom Koulopoulos, part 2 2IT outsourcing according to Tom Koulopoulos, part 2 3IT outsourcing according to Tom Koulopoulos, part 2 4UW shows why avian flu hasn't spread among humans yet 2UW shows why avian flu hasn't spread among humans yet 3
Cyno macaque plasma and serum - various quantities available...
3'-O-Methylguanosine 5'-Triphosphate, Sodium, 1 umol. Category: Nucleotides & Enzymes & Biochemicals, Nucleotides, Additional Nucleotide Products....
EPH electrode, cathode, 1. Category: Electrophoresis Systems & Standards & Reagents, IEF & SDS-PAGE & 2-D Electrophoresis, Multiphor II Kits & Components and Accessories....
Rat Normal Multiple Tissue Array 1 slide...
Biology Products: