"These (proposal) selections truly take advantage of the DOE JGI's massive-scale sequencing and data analysis capabilities," said Eddy Rubin, DOE JGI director. "The projects span the globe and the unexplored branches of the tree of life, and promise to yield a better understanding of the interplay between climate, ecosystem and organism."
The 2012 Community Sequencing Program call invited researchers to submit proposals for projects that advance capabilities in fields such as microbes involved in carbon capture and greenhouse gas emission, and metagenomics -- the characterization of complex collections of microbes from particular environmental niches.
For the BGSU team, that niche includes the hypolimnion, or deepest layer of the water column at the bottom of the lake, which is not mixed by the wind and can produce significant amounts of greenhouse gases during low-oxygen periods caused by microbial nitrate sinks and other factors.
Bullerjahn, McKay and Morris's new project will involve sampling at the site of a NOAA buoy that continuously monitors oxygen and temperature through the water column during summer and fall. They will also use samples taken from the hypolimnion and the lake sediments before and after the onset of low oxygen conditions in the central basin. The RNA and DNA sequencing of microbes from those samples should provide a trove of informatio
|Contact: Jen Sobolewski|
Bowling Green State University