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BGI releases a complete de novo E. coli O104 genome and details of their detection kit
Date:6/7/2011

P site). Diagnostic results can be obtained within 2𔃁 hours after receiving the sample, and thus will be extremely useful for epidemic surveillance and detection of this bacterium.

BGI has assessed the specificity and sensitivity of this kit and protocol through computational analyses of 4,547 strains (from 2,183 species) using publicly available whole-genome sequences, and through experimental analyses of 323 DNA samples (from 93 species, including 55 E. coli strains that have different phenotypes and the current infectious strain). The findings demonstrated that the kit and protocol have high specificity: no bacterial strain other than E. coli O104 had positive amplification results of both target regions. Sensitivity testing indicated that the kit and protocol could detect this bacterium using a DNA concentration as low as ~1 picogram (10-12 g) in the PCR. Additional validation tests on more patient isolates will be carried out within the week.


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Contact: Bicheng Yang
yangbicheng@genomics.cn
Beijing Genomics Institute at Shenzhen
Source:Eurekalert

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