Navigation Links
BGI develops RNA-Seq (Quantification) from as low as 100 Ng total RNA

September 20th, 2011,Shenzhen, China BGI, the world's largest genomics organization, reported that they have achieved optimization RNA-Seq (Quantification) library construction with total RNA inputs as low as 100 ng. This breakthrough enables the application of RNA-Seq (Quantification) technology to experimental designs utilizing samples derived from small numbers of cells, such as those widely used in pharmaceutical research, cancer research, and immunology.

RNA-Seq (Quantification), a version of Next Generation Sequencing (NGS), is used for transcriptome quantification and analyzing the gene expression of certain biological objects in specific conditions. It can be widely applied in biomarker detection, basic medical research, drug discovery, among others. Compared with microarray technology, high-throughput RNA sequencing can provide comprehensive assessment of RNA expression profiles with the advantages of high-throughput data, low background, high sensitivity and repeatability.

However, some tissues or cultures from specialized cells involved in clinical and pharmaceutical research make it difficult to obtain sufficient RNA for RNA-Seq (Quantification), which previously required 1 μg or more total RNA. BGI has optimized the procedures to enable RNA-Seq (Quantification) using as little as 100 ng total RNA sample input to generate high-quality data. "The improvement of RNA-Seq (Quantification) not only can simplify sample preparation, but also make this sequencing service more cost-effective and with rapid turnaround time," said Jiong Zhang, Technical Specialist at BGI.

To ensure the accuracy and quality of data, many evaluations have been conducted at BGI, including the reads quality, assessments of reads randomness, gene coverage, experimental reproducibility, and data accuracy. Results demonstrated that the data generated from 100 ng sample input library was as high-quality as that from traditional 1 μg RNA input library. "I hope our enhanced technique can contribute more to drug discovery and therapeutic application in the future," added Zhang.


Contact: Liu Jia
Beijing Genomics Institute

Related biology news :

1. UConn scientist develops sterile variety of invasive plant
2. BGI develops new strategy to uncover structural variations of human genomes
3. How muscle develops: A dance of cellular skeletons
4. New research provides insight into how OCD develops
5. UBC-led team develops platform to monitor hematopoietic stem cells
6. NJIT professor develops a biologically inspired catalyst, an active yet inert material
7. NC State develops material to remove radioactive contaminants from drinking water
8. Advertising Research Foundation Develops Standards for Neuromarketing Research
9. Syracuse University team develops functionally graded shape memory polymers
10. WebLib develops application for Elseviers SciVerse applications
11. Researcher develops accurate method for detecting dangerous fluoride
Post Your Comments:
(Date:11/2/2015)...  SRI International has been awarded a contract of ... to the National Cancer Institute (NCI) PREVENT Cancer Program ... modern testing and support facilities, and analytical instrumentation to ... studies to evaluate potential cancer prevention drugs. ... Drug Development Program is an NCI-supported pipeline to bring ...
(Date:10/29/2015)... 29, 2015  The J. Craig Venter Institute (JCVI) ... Synthesis and Biosecurity: Lessons Learned and Options for the ... and Human Services guidance for synthetic biology providers has ... --> --> Synthetic biology ... potential to pose unique biosecurity threats. It now is ...
(Date:10/29/2015)... ANN ARBOR, Mich. , Oct. 29, 2015 ... with Eurofins Genomics for U.S. distribution of its ... DNA-seq kit and Rubicon,s new ThruPLEX Plasma-seq ... DNA to enable the preparation of NGS libraries ... in plasma for diagnostic and prognostic applications in ...
Breaking Biology News(10 mins):
(Date:11/24/2015)... Mass. (PRWEB) , ... November 24, 2015 , ... ... to maintain healthy metabolism. But unless it is bound to proteins, copper is ... of Health (NIH), researchers at Worcester Polytechnic Institute (WPI) will conduct a systematic ...
(Date:11/24/2015)... -- Clintrax Global, Inc., a worldwide provider of clinical research services headquartered ... the company has set a new quarterly earnings record in Q3 ... posted for Q3 of 2014 to Q3 of 2015.   ... , with the establishment of an Asia-Pacific ... United Kingdom and Mexico , with ...
(Date:11/24/2015)... ... , ... The Academy of Model Aeronautics (AMA), led by its Executive Council, ... Grand Prix, to represent the First–Person View (FPV) racing community. , FPV racing has ... type of racing and several new model aviation pilots have joined the community because ...
(Date:11/24/2015)... Israel , Nov. 24, 2015  Tikcro Technologies Ltd. (OTCQB: TIKRF) ... on December 29, 2015 at 11:00 a.m. Israel ... Electra Tower, 98 Yigal Allon Street, 36 th Floor, ... of Eric Paneth and Izhak Tamir to the ... Rami Skaliter as external directors; , approval of an amendment to ...
Breaking Biology Technology: