Navigation Links
BGI develops RNA-Seq (Quantification) from as low as 100 Ng total RNA
Date:9/20/2011

September 20th, 2011,Shenzhen, China BGI, the world's largest genomics organization, reported that they have achieved optimization RNA-Seq (Quantification) library construction with total RNA inputs as low as 100 ng. This breakthrough enables the application of RNA-Seq (Quantification) technology to experimental designs utilizing samples derived from small numbers of cells, such as those widely used in pharmaceutical research, cancer research, and immunology.

RNA-Seq (Quantification), a version of Next Generation Sequencing (NGS), is used for transcriptome quantification and analyzing the gene expression of certain biological objects in specific conditions. It can be widely applied in biomarker detection, basic medical research, drug discovery, among others. Compared with microarray technology, high-throughput RNA sequencing can provide comprehensive assessment of RNA expression profiles with the advantages of high-throughput data, low background, high sensitivity and repeatability.

However, some tissues or cultures from specialized cells involved in clinical and pharmaceutical research make it difficult to obtain sufficient RNA for RNA-Seq (Quantification), which previously required 1 μg or more total RNA. BGI has optimized the procedures to enable RNA-Seq (Quantification) using as little as 100 ng total RNA sample input to generate high-quality data. "The improvement of RNA-Seq (Quantification) not only can simplify sample preparation, but also make this sequencing service more cost-effective and with rapid turnaround time," said Jiong Zhang, Technical Specialist at BGI.

To ensure the accuracy and quality of data, many evaluations have been conducted at BGI, including the reads quality, assessments of reads randomness, gene coverage, experimental reproducibility, and data accuracy. Results demonstrated that the data generated from 100 ng sample input library was as high-quality as that from traditional 1 μg RNA in
'/>"/>

Contact: Liu Jia
liujia@genomics.cn
Beijing Genomics Institute
Source:Eurekalert

Page: 1 2

Related biology news :

1. UConn scientist develops sterile variety of invasive plant
2. BGI develops new strategy to uncover structural variations of human genomes
3. How muscle develops: A dance of cellular skeletons
4. New research provides insight into how OCD develops
5. UBC-led team develops platform to monitor hematopoietic stem cells
6. NJIT professor develops a biologically inspired catalyst, an active yet inert material
7. NC State develops material to remove radioactive contaminants from drinking water
8. Advertising Research Foundation Develops Standards for Neuromarketing Research
9. Syracuse University team develops functionally graded shape memory polymers
10. WebLib develops application for Elseviers SciVerse applications
11. Researcher develops accurate method for detecting dangerous fluoride
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:8/27/2014)... Outstanding basic research, a growing focus on ... patient care have earned the Herbert Irving Comprehensive ... (CUMC) and NewYork-Presbyterian Hospital an $18 million, five-year ... Institute (NCI). The grant renews the center,s status ... Centers in New York City and one of ...
(Date:8/27/2014)... has shown that piglets can be weaned later with ... outcome of the study at the University,s Roseworthy campus, ... is an important finding for pig producers. It allows ... production. , "Sows don,t usually start their oestrous cycles ... piglets have been weaned," says Ms Alice Weaver, PhD ...
(Date:8/26/2014)... in St. Louis have developed algorithms to identify weak ... or breaking. The technology, which needs to be refined ... help pinpoint minor strains and tiny injuries in the ... research is available online Aug. 27 in the ... research at the nexus of the physical and life ...
Breaking Biology News(10 mins):Herbert Irving Comprehensive Cancer Center awarded $18 million grant 2Herbert Irving Comprehensive Cancer Center awarded $18 million grant 3Herbert Irving Comprehensive Cancer Center awarded $18 million grant 4Piglet weaning age no bar to litter frequency 2New technology may identify tiny strains in body tissues before injuries occur 2New technology may identify tiny strains in body tissues before injuries occur 3
... at The Scripps Research Institute and collaborating institutions has identified ... a target for obesity and diabetes drugs. In a ... , the scientists describe a protein called TLE3 that acts ... fat cell formation and turn off those that keep fat ...
... With the Easter holiday season coming up soon, egg consumption ... farms producing the maximum number of eggs they can? "Parasites ... production by five percent or more," says Bradley A. Mullens ... California, Riverside. "One way to boost egg production is to ...
... among a group of researchers investigating how genetic differences ... interact and change over time. They say ... can help society to address issues including food security, ... nature in a crowded world. Biologists from the ...
Cached Biology News:Scripps Research scientists find 'dual switch' regulates fat formation 2Scripps Research scientists find 'dual switch' regulates fat formation 3UC Riverside entomologists propose pesticide-free method to increase egg production 2Genetic differences influence the structure of communities 2
(Date:8/27/2014)... Remcom announces an update to XFdtd® ... the biomedical industry. The central biomedical addition in ... to the Bio-Pro version of the software. XFdtd ... calculate the biological effects of electromagnetic fields. In ... versions to expand engineers’ post-processing options. , The Biological ...
(Date:8/26/2014)... August 26, 2014 New research ... wearable technology, and inspiration for the International Year ... year’s brighter, busier SPIE Optics + Photonics ... More than 4,400 international scientists, engineers, researchers, developers, ... exhibition, and industry sessions sponsored by SPIE, ...
(Date:8/26/2014)... Israel , August 26, 2014 ... supports NHS hospitals with surgery validation using its Stratasys Objet24 ... - Performing surgery on 3D printed models prior to operations ... provides an effective aid for both patient and surgeon ... SSYS ), a global leader of 3D printing and additive ...
(Date:8/26/2014)... The global market for molecular diagnostics ... according to a new study by Grand View Research, ... the subsequent introduction of advanced cancer diagnostic technologies are ... the next six years. Moreover, the growing global base ... coupled with disease triggering lifestyle habits such as smoking ...
Breaking Biology Technology:Remcom Announces Enhanced Biological Thermal Sensor and Other Biomedical Features in XFdtd EM Simulation Software Update 2Remcom Announces Enhanced Biological Thermal Sensor and Other Biomedical Features in XFdtd EM Simulation Software Update 3Brighter, Busier SPIE Optics + Photonics Draws International Crowd to San Diego 2Brighter, Busier SPIE Optics + Photonics Draws International Crowd to San Diego 3Brighter, Busier SPIE Optics + Photonics Draws International Crowd to San Diego 4Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 2Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 3Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 4Twelve UK Hospitals Enjoy Reduced Surgical Procedure Times and Decline in Costs by Implementing Stratasys 3D Printing Solutions 5World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 2World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 3World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 4World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 5World Molecular Diagnostics Market Is Expected To Grow To $8,020.1 Million From 2014 To 2020: Grand View Research, Inc 6
... , April 22 As evidence of ... lifetime of physical activity, Smith & Nephew ( SNN, ... unprecedented testing on its VERILAST technology for knee replacement ... the company,s LEGION™ knee replacement, VERILAST technology produced an ...
... YORK and SCOTTSDALE, Ariz. , April ... NBS ), an international biopharmaceutical,company with operations in the ... wholly owned subsidiary of IR BioSciences Holdings, Inc.,(OTC Bulletin ... a,Collaborative Research Agreement, focused on the development of an ...
... ... Follow-on Biologics in Chicago. , ... Chicago (Vocus) April 22, 2010 -- Therapeutic Proteins Inc. (TPI) ... manufacturing facility constructed to meet U.S. Food and Drug Administration (FDA) requirements. The Chicago ...
Cached Biology Technology:Smith & Nephew Receives FDA 510k Clearance for a 30-year Knee 2Smith & Nephew Receives FDA 510k Clearance for a 30-year Knee 3Smith & Nephew Receives FDA 510k Clearance for a 30-year Knee 4Smith & Nephew Receives FDA 510k Clearance for a 30-year Knee 5NeoStem, Inc. and ImmuneRegen BioSciences(R) Announce a Collaborative Agreement to Advance Stem Cell Technology 2NeoStem, Inc. and ImmuneRegen BioSciences(R) Announce a Collaborative Agreement to Advance Stem Cell Technology 3NeoStem, Inc. and ImmuneRegen BioSciences(R) Announce a Collaborative Agreement to Advance Stem Cell Technology 4NeoStem, Inc. and ImmuneRegen BioSciences(R) Announce a Collaborative Agreement to Advance Stem Cell Technology 5NeoStem, Inc. and ImmuneRegen BioSciences(R) Announce a Collaborative Agreement to Advance Stem Cell Technology 6NeoStem, Inc. and ImmuneRegen BioSciences(R) Announce a Collaborative Agreement to Advance Stem Cell Technology 7Therapeutic Proteins Inc. Completes First Independent, Dedicated Biosimilars Manufacturing Facility in U.S. 2Therapeutic Proteins Inc. Completes First Independent, Dedicated Biosimilars Manufacturing Facility in U.S. 3Therapeutic Proteins Inc. Completes First Independent, Dedicated Biosimilars Manufacturing Facility in U.S. 4
Request Info...
... Nuclease catalyzes the degradation of single-stranded DNA ... products. While the nuclease prefers ssDNA over ... the enzyme degrades double-stranded DNA from both ... used for transcript mapping studies (3,4), for ...
... fosmid (40kb), and large plasmid insert (10-20kb) ... BAC vector. On command 20-50 fold copy ... DNA purification. Available with blue-white screening (pEZ ... the highest insert stability. Kits include electrocompetent ...
...
Biology Products: