Navigation Links
Army study: DNA vaccine and duck eggs protect against hantavirus disease
Date:5/23/2012

Army scientists and industry collaborators have successfully protected laboratory animals from lethal hantavirus disease using a novel approach that combines DNA vaccines and duck eggs. The work appears in a recent edition of the online scientific journal PLoS ONE, published by the Public Library of Science.

According to first author Jay W. Hooper of the U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), this is the first time that the DNA vaccine/duck egg system has been shown to produce an antiviral product capable of protecting against hantavirus disease.

Hantavirus causes a condition known as hantavirus pulmonary syndrome (HPS), which has a case fatality rate of 35-40 percent. Currently there are no vaccines, prophylactics, or therapeutics to prevent or treat this highly pathogenic disease.

In this study, the research team used a hamster model of Andes virus, which is the predominant cause of HPS in South America and the only hantavirus known to be transmitted person-to-person. Infection of Syrian hamsters with Andes virus, as demonstrated in earlier studies at USAMRIID, results in a disease that closely mimics human HPS in incubation time, symptoms of respiratory distress, and disease pathology. This makes it an ideal system for evaluating the feasibility of postexposure protection strategies.

Collaborating with Aldevron of Fargo, N.D. and the Universidad del Desarrollo in Santiago, Chile, Hooper and his team first evaluated a natural product, human polyclonal antibody, which was obtained as fresh frozen plasma (FFP) from a patient who survived HPS. Their results indicate that FFP shows promise as a post-exposure preventive treatment for HPS.

The team then vaccinated ducks with a DNA vaccine against Andes virus. This vaccine, initially developed and tested at USAMRIID, uses genetic material, or DNA, that encodes a specific hantavirus gene to elicit an immune response in the recipient.

Next, they purified an antibody called IgY from the yolks of the duck eggs. This purified IgY, as well as a similar version produced in duck eggs, was capable of neutralizing Andes virus when tested in cell culture. More importantly, it also protected Syrian hamsters from lethal HPSeven when administered as a single injection several days after the hamsters had been exposed to a lethal dose of virus.

The work demonstrates the feasibility of using DNA vaccine technology, coupled with the duck/egg system, to manufacture a product that could supplement or replace FFP. Furthermore, the new approach can be scaled as needed and eliminates the necessity of using blood products from HPS survivors, which may be in limited supply.

According to Hooper, another advantage of this technique is that duck IgY naturally loses a part of the antibody that has been associated with "serum sickness" when animal antibodies have been used in humans, making the product potentially less reactogenic.

"This antiviral product, if fully developed and manufactured, has the potential to be used in future outbreak situations," Hooper said. "It also could be used to treat health care workers and others who have close contact with HPS patients."

In addition, the authors suggest, the flexibility of the DNA vaccine/duck egg system could be applied to the production of antibodies against other infectious agents and toxins.


'/>"/>
Contact: Caree Vander Linden
caree.vanderlinden@us.army.mil
301-619-2285
US Army Medical Research Institute of Infectious Diseases
Source:Eurekalert

Related biology news :

1. Study: urban black bears live fast, die young
2. Study: Bird diversity lessens human exposure to West Nile Virus
3. Study: Tropical wetlands hold more carbon than temperate marshes
4. Study: Wildlife need more complex travel plans
5. Study: Elderly Women can increase strength but still risk falls
6. UNC study: Text messaging may help children fight off obesity
7. Study: Did early climate impact divert a new glacial age?
8. Study: Excessive use of antiviral drugs could aid deadly flu
9. UNC study: Tinkering with the circadian clock can suppress cancer growth
10. Study: Fluid buildup in lungs is part of the damage done by the flu
11. Study: Health undervalued in reproductive rights debate
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:3/15/2016)... 15, 2016 Yissum Research Development Company ... company of the Hebrew University, announced today the formation ... technology of various human biological indicators. Neteera Technologies has ... from private investors. ... detection of electromagnetic emissions from sweat ducts, enables reliable ...
(Date:3/10/2016)... PUNE, India , March 10, 2016 ... to a new market research report "Identity and Access ... SSO, & Audit, Compliance, and Governance), by Organization Size, ... Forecast to 2020", published by MarketsandMarkets, The market is ... to USD 12.78 Billion by 2020, at a Compound ...
(Date:3/8/2016)... N.C. , March 8, 2016   ... sensor technology, today announced it has secured $11M ... by GII Tech, a new venture fund being ... with additional participation from existing investors TDF Ventures ... the funds to continue its triple-digit growth and ...
Breaking Biology News(10 mins):
(Date:5/26/2016)... ... May 26, 2016 , ... ... are used in leading laboratories all over the globe. Their cute firefly logo ... to manufacturing awesome cuvettes, FireflySci makes spectrophotometer calibration standards that never require recalibration. ...
(Date:5/25/2016)... ... May 25, 2016 , ... Thailand’s ... at BIO 2016 in San Francisco. Located at booth number 7301, representatives from ... answer questions and discuss the Thai biotechnology and life sciences sector. , ...
(Date:5/25/2016)... Phoenix, AZ (PRWEB) , ... May 25, 2016 ... ... Arizona, Dr. Michael Fitzmaurice recently became double board-certified in surgery and surgery of ... 31, 2015. Dr. Fitzmaurice is no stranger to going above and beyond in ...
(Date:5/25/2016)... ... 25, 2016 , ... Scientists at the University of Athens say they have ... be hampering the research that could lead to one good one. Surviving Mesothelioma has ... now. , The team evaluated 98 mesothelioma patients who got a ...
Breaking Biology Technology: