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Argonne-University of Chicago joint venture bolsters genomic sequencing capabilities
Date:6/9/2008

ARGONNE, Ill. (June 9, 2008) The Institute for Genomics and Systems Biology (IGSB), a joint venture of the U.S. Department of Energy's (DOE) Argonne National Laboratory and the University of Chicago, has acquired two new instruments that provide an enhanced ability to sequence genomes more quickly and broadly.

"Sequencing used to be like locating a golf ball by searching only on the fairway, but not the rough," said IGSB Director Kevin White. "It used to be that only species that could be cultivated, or grown in pure culture, could be sequenced. The capabilities of the new Roche 454 FLEX and Illumina Solexa Genome Sequencer now allow scientists that use the machines to skip the cultivation step. Eliminating that step will save time and speed up the research process, while maintaining accurate sequencing results."

The 454 FLEX is ideally suited for studying microbial communities by de novo sequencing. It provides 400,000 DNA fragments of about 250 base pairs each or 100 million base pairs per run that represent either a significant part of the genome of a single organism or a random snapshot of parts of multiple genomes.

The Solexa Genome Sequencer is targeted at resequencing. Compared to the Roche 454 FLEX, it generates more but shorter reads, creating 40 million reads with a current read length of 18 to 36 base pairs or about 1 billion base pairs per run depending on the application.

The machines were purchased to facilitate research for three Argonne Laboratory-Directed Research and Development projects. A project led by Michael Miller, a terrestrial ecologist, and Folker Meyer, a computational biologist in IGSB, will enhance our understanding of soil CO2 sequestration capability on the microbial level.

In another project, Argonne's soil ecology group is using metagenome sequencing to study the microbial population in chronoseries plots at DOE's Fermi National Accelerator Laboratory. In a third
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Contact: Angela Hardin
ahardin@anl.gov
630-252-5501
DOE/Argonne National Laboratory
Source:Eurekalert

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