Navigation Links
Argonne-University of Chicago joint venture bolsters genomic sequencing capabilities
Date:6/9/2008

ARGONNE, Ill. (June 9, 2008) The Institute for Genomics and Systems Biology (IGSB), a joint venture of the U.S. Department of Energy's (DOE) Argonne National Laboratory and the University of Chicago, has acquired two new instruments that provide an enhanced ability to sequence genomes more quickly and broadly.

"Sequencing used to be like locating a golf ball by searching only on the fairway, but not the rough," said IGSB Director Kevin White. "It used to be that only species that could be cultivated, or grown in pure culture, could be sequenced. The capabilities of the new Roche 454 FLEX and Illumina Solexa Genome Sequencer now allow scientists that use the machines to skip the cultivation step. Eliminating that step will save time and speed up the research process, while maintaining accurate sequencing results."

The 454 FLEX is ideally suited for studying microbial communities by de novo sequencing. It provides 400,000 DNA fragments of about 250 base pairs each or 100 million base pairs per run that represent either a significant part of the genome of a single organism or a random snapshot of parts of multiple genomes.

The Solexa Genome Sequencer is targeted at resequencing. Compared to the Roche 454 FLEX, it generates more but shorter reads, creating 40 million reads with a current read length of 18 to 36 base pairs or about 1 billion base pairs per run depending on the application.

The machines were purchased to facilitate research for three Argonne Laboratory-Directed Research and Development projects. A project led by Michael Miller, a terrestrial ecologist, and Folker Meyer, a computational biologist in IGSB, will enhance our understanding of soil CO2 sequestration capability on the microbial level.

In another project, Argonne's soil ecology group is using metagenome sequencing to study the microbial population in chronoseries plots at DOE's Fermi National Accelerator Laboratory. In a third project, Argonne's environmental remediation program is studying the role played by microbial communities in subsurface remediation of inorganic contaminates using metagenome sequencing.

IGSB's sequencing group plays an active role in the design and optimization of experiments using DNA sequencing technology, such as developing and optimizing protocols for DNA isolation from environment as diverse as subsurface soil and plant leaves. The group also works with researchers to develop protocols for DNA extraction and to conduct downstream bioinformatics analyses.

The new machines are also open to other Argonne and University of Chicago researchers who need genetic samples sequenced. In the near future, the sequencing instruments will be available to select peer-reviewed proposals from researchers from other organizations.


'/>"/>

Contact: Angela Hardin
ahardin@anl.gov
630-252-5501
DOE/Argonne National Laboratory
Source:Eurekalert

Related biology news :

1. NIH awards nearly $23M to University of Chicago for translational research
2. UC San Diego begins trading greenhouse gas credits on Chicago Climate Exchange
3. University of Chicago launches research program in catastrophic deformation
4. DHS Begins Collecting 10 Fingerprints From International Visitors at Chicago OHare International Airport
5. Pitt and University of Chicago researchers uncover process behind heart muscle contraction
6. First-ever study: lack of critical lubricant causes wear in joints
7. Monsanto expands sponsorship for Peking-Yale Joint Agrobiotechnology Center
8. Experts from Stevens, Merck, publish joint paper, Biosynthetic Studies of Platensimycin
9. Roger Kornberg to present lecture at the Joint Biophysical Society/IUPAB Meeting
10. Electronic switch opens doors in rheumatoid joints
11. Mechanisms of plant-fungi symbiosis characterized by DOE Joint Genome Institute
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:2/14/2017)... Feb. 14, 2017  Wake Forest Baptist Medical Center ... new chief executive officer (CEO). Freischlag joins the medical ... D. McConnell , M.D., who last year announced that ... Medical Center, after leading it since 2008.   ... of Wake Forest Baptist,s academic health system, which includes ...
(Date:2/13/2017)... 2017 Former 9/11 Commission border counsel and ... Janice Kephart of Identity Strategy Partners, LLP, today ... "Executive Order: Protecting the Nation From Foreign Terrorist ... "As President Trump,s ,Travel Ban, Executive Order gains ... banned the travel ban, it is important that our ...
(Date:2/9/2017)... LONDON , Feb. 9, 2017 The ... in-depth analysis of the biomass boiler market globally in ... sales of biomass boilers. The market for biomass boilers ... product type, end-user, application, and country/region. The market based ... agriculture & forest residues, biogas & energy crops, urban ...
Breaking Biology News(10 mins):
(Date:2/24/2017)... 24, 2017  OncoSec Medical Incorporated ("OncoSec") (NASDAQ: ONCS), ... a Key Opinion Leader event to highlight new clinical ... poster presentation at the upcoming 2017 ASCO-SITC Immuno-Oncology Symposium ... will be held in-person and via live webcast on ... AM PST at the Lotte New York Palace Hotel ...
(Date:2/23/2017)... - The Fight Against Cancer Innovation Trust (FACIT) and ... to report that Fusion Pharmaceuticals Inc. (Fusion) has closed ... Innovation – JJDC, Inc. (JJDC) as the lead investor. ... Partners, and Genesys Capital, as well as founding investor ... ...
(Date:2/23/2017)... Feb. 23, 2017  Imanis Life Sciences announced ... of oncolytic vaccinia viruses for virotherapy research. These ... of Genelux,s proprietary, vaccinia virus-based technology platform for ... enter into a partnership with Genelux to offer ... viruses for use in research," said Dr. ...
(Date:2/23/2017)... ... February 23, 2017 , ... ... as insulin, cortisol, CRP, adiponectin, uric acid, and/or other biomarkers or SNPs of ... Assay from Salimetrics’ SalivaLab , the relationship between insulin and other relevant ...
Breaking Biology Technology: