Navigation Links
Antibacterial protein's molecular workings revealed
Date:2/21/2013

bind manganese would be useful tools for determining the importance of manganese binding to calprotectin's functions, Chazin noted.

Thomas Kehl-Fie, Ph.D., a postdoctoral fellow in Skaar's group, used these altered calprotectins to demonstrate that the protein's ability to bind manganese is required for full inhibition of Staphylococcus aureus growth. The investigators also showed that Staph bacteria require manganese for a certain process the bacteria use to protect themselves from reactive oxygen species.

"These altered calprotectin proteins were key to being able to tease apart the importance of the individual metals zinc and manganese to the bacterium as a whole and to metal-dependent processes within the bacteria," Skaar said. "They're really powerful tools."

Skaar explained that calprotectin likely binds two different metals to increase the range of bacteria that it inhibits. The investigators tested the modified calprotectins against a panel of medically important bacterial pathogens.

"Bacteria have different metal needs," Skaar said. "Some bacteria are more sensitive to the zinc-binding properties of calprotectin, and others are more sensitive to the manganese-binding properties."

To fully understand how calprotectin binds manganese, Damo and Chazin with assistance from Gnter Fritz, Ph.D., at the University of Freiburg in Germany produced calprotectin crystals with manganese bound and determined the protein structure. They found that manganese slips into a position where it interacts with six histidine amino acids of calprotectin.

"It's really beautiful; no one's ever seen a protein chelate (bind) manganese like this," Chazin said.

The structure explains why calprotectin is the only S100 family member that binds manganese and has the strongest antimicrobial action, and it may allow researchers to design a calprotectin that only binds manganese (not zinc). Such a tool would be useful for studying why bacteria require m
'/>"/>

Contact: Leigh MacMillan
leigh.macmillan@vanderbilt.edu
615-322-4747
Vanderbilt University Medical Center
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Antibacterial agent used in common soaps found in increasing amounts in freshwater lakes
2. Study quantifies the size of holes antibacterials create in cell walls to kill bacteria
3. Nano-machines for bionic proteins
4. Discovering cell surface proteins behavior
5. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
6. FASEB SRC announces conference: Matricellular Proteins in Development, Health, and Disease
7. First special edition updating progress on efforts to map human proteins
8. NYU biologists identify proteins vital to chromosome segregation
9. Cholesterol helps regulate key signaling proteins in the cell
10. Researchers identify proteins that indicate which kidney tumors are most likely to spread
11. Using computational biology for the annotation of proteins
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:12/10/2014)... You,ve been here before: you desperately need to sign into ... key or the answer to your secret question. What,s your ... Today, Hoyos Labs , a digital infrastructure security ... an end to the frustration that comes with usernames, passwords ... a user,s smartphone to acquire his or her biometrics to ...
(Date:12/10/2014)... 08, 2014 Research and Markets ... the "Biometrics Market in Japan 2014-2018" ... http://photos.prnewswire.com/prnh/20130307/600769 The integration of ... rural banking and upgradation of the driver,s license ... the market. Besides the aforementioned projects, biometrics is ...
(Date:12/4/2014)... GARDENS, Fla. , Dec. 3, 2014 /PRNewswire/ ... solutions, today announced that its DigitalPersona ® ... fingerprint readers have been deployed throughout First Bank ... , South Carolina and ... in Southern Pines, North Carolina ...
Breaking Biology News(10 mins):The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 2The Password is Finally Dead: Launch of 1U Mobile App Eliminates Need for All Usernames and Passwords 3Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3
... survival strategy of the parasite that causes sleeping sickness could ... found that the parasite, which can transform itself into either ... these. One of these types ensures infection in the bloodstream ... by the tsetse fly and spread to another person or ...
... is available in German . It would ... would have similar genetic blueprints. However, scientists from the Max ... with an international research team have now decoded, for the ... ( Arabidopsis lyrata ), a close relative of the thale ...
... release is available in Spanish . ... Agriculture (USDA) scientists and colleagues can improve polymerase chain ... PCR-based tests are prized tools for diagnosing plant ... among other economic harm. But the test,s ability to ...
Cached Biology News:Evolution can cause a rapid reduction in genome size 2Evolution can cause a rapid reduction in genome size 3New technique improves sensitivity of PCR pathogen detection 2
(Date:12/19/2014)... CA (PRWEB) December 18, 2014 With ... limited time special on Sartorius Biohit products . ... on mLINE pipettes, Picus Electronic Pipette Trade-in Program, and ... Health and Safety officers around the world. They are ... pipetting. Other features include:, ,     Full volume ...
(Date:12/19/2014)... Dec. 18, 2014   ISN has released ... Performance Indicators publication series. With data aggregated ... reported in 2013 within its online contractor management platform, ... key performance indicator (KPI) statistics. The ... publication is available in two industry specific ...
(Date:12/17/2014)... 2014 Based on the revenues ... imaging market is dominated by four major players ... market. These players include GE Healthcare (U.K.), Siemens ... , Full Report Copy at http://www.marketsandmarkets.com/Market-Reports/surgical-imaging-market-210534462.html ... can be attributed to its strong product portfolio, ...
(Date:12/17/2014)... N.J. (PRWEB) December 17, 2014 ... (Euronext: IPN; ADR: IPSEY), today announced ... (referred to as Somatuline®) was approved by the ... treatment of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) in adult ... advanced or metastatic disease to improve progression-free survival ...
Breaking Biology Technology:Major Pipette Distributor Pipette.com Announces Sartorius Year End Specials 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 2ISN Releases Third Edition of US Contractor Health & Safety Performance Indicators Publication Series 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 2Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 3Global Refurbished Surgical Imaging Market by Product & Application is Estimated to Reach $1.25 Billion by 2019 - New Research Report by MarketsandMarkets 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 8
... Development of Nano-Materials for Angiogenesis Applications , ... OmegaGenesis announced today that they have entered into a ... nano-materials used for Angiogenesis applications. , , ... variety of medical applications ranging from tissue reactivation and healing ...
... 3 Combination Rule Trial for NDA Submission , ... N.J., Dec. 15 QRxPharma Limited (ASX: QRX and ... the development and commercialization of therapies for pain and ... a comparative study to evaluate the efficacy and safety ...
... N.Y., Dec. 15 Allscripts,announced today that ... multispecialty group practice, has selected Allscripts,Practice Management ... for their 190 physicians. , ... ) , ...
Cached Biology Technology:OmegaGenesis Announces Angiogenesis Application Collaboration 2OmegaGenesis Announces Angiogenesis Application Collaboration 3QRxPharma Initiates Comparative Study for MoxDuoIR(TM) Dual-Opioid(TM) Pain Therapy 2QRxPharma Initiates Comparative Study for MoxDuoIR(TM) Dual-Opioid(TM) Pain Therapy 3Community Care Physicians Selects Allscripts Practice Management System for 190 Physicians in New York Capitol Region 2Community Care Physicians Selects Allscripts Practice Management System for 190 Physicians in New York Capitol Region 3Community Care Physicians Selects Allscripts Practice Management System for 190 Physicians in New York Capitol Region 4Community Care Physicians Selects Allscripts Practice Management System for 190 Physicians in New York Capitol Region 5
Anti-DDDDK-Tag (Enterokinase Cleavage Site), Agarose Conjugate Research Focus: tag Storage: 4C Shipping Temperature: 4C...
Goat polyclonal to XAGE1 ( Abpromise for all tested applications). Antigen: Synthetic peptide: CGFGFRRQGEDNT, corresponding to C terminal amino acids 149-160 of Human XAGE1 Entrez Gene ID: 9...
Sheep polyclonal to Staphylococcus DNase ( Abpromise for all tested applications). Antigen: Full length native protein (purified)....
Mouse monoclonal [MN-8C4] to zona radiata ( Abpromise for all tested applications). Antigen: Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from ...
Biology Products: