Navigation Links
Antibacterial protein's molecular workings revealed
Date:2/21/2013

bind manganese would be useful tools for determining the importance of manganese binding to calprotectin's functions, Chazin noted.

Thomas Kehl-Fie, Ph.D., a postdoctoral fellow in Skaar's group, used these altered calprotectins to demonstrate that the protein's ability to bind manganese is required for full inhibition of Staphylococcus aureus growth. The investigators also showed that Staph bacteria require manganese for a certain process the bacteria use to protect themselves from reactive oxygen species.

"These altered calprotectin proteins were key to being able to tease apart the importance of the individual metals zinc and manganese to the bacterium as a whole and to metal-dependent processes within the bacteria," Skaar said. "They're really powerful tools."

Skaar explained that calprotectin likely binds two different metals to increase the range of bacteria that it inhibits. The investigators tested the modified calprotectins against a panel of medically important bacterial pathogens.

"Bacteria have different metal needs," Skaar said. "Some bacteria are more sensitive to the zinc-binding properties of calprotectin, and others are more sensitive to the manganese-binding properties."

To fully understand how calprotectin binds manganese, Damo and Chazin with assistance from Gnter Fritz, Ph.D., at the University of Freiburg in Germany produced calprotectin crystals with manganese bound and determined the protein structure. They found that manganese slips into a position where it interacts with six histidine amino acids of calprotectin.

"It's really beautiful; no one's ever seen a protein chelate (bind) manganese like this," Chazin said.

The structure explains why calprotectin is the only S100 family member that binds manganese and has the strongest antimicrobial action, and it may allow researchers to design a calprotectin that only binds manganese (not zinc). Such a tool would be useful for studying why bacteria require m
'/>"/>

Contact: Leigh MacMillan
leigh.macmillan@vanderbilt.edu
615-322-4747
Vanderbilt University Medical Center
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Antibacterial agent used in common soaps found in increasing amounts in freshwater lakes
2. Study quantifies the size of holes antibacterials create in cell walls to kill bacteria
3. Nano-machines for bionic proteins
4. Discovering cell surface proteins behavior
5. FASEB SRC announces conference registration open for: Arf and Rab Family G Proteins
6. FASEB SRC announces conference: Matricellular Proteins in Development, Health, and Disease
7. First special edition updating progress on efforts to map human proteins
8. NYU biologists identify proteins vital to chromosome segregation
9. Cholesterol helps regulate key signaling proteins in the cell
10. Researchers identify proteins that indicate which kidney tumors are most likely to spread
11. Using computational biology for the annotation of proteins
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/13/2015)... 2015 Synaptics Inc. (NASDAQ: SYNA ... today announced sampling of ClearPad ® 4300, ... (TDDI) product targeting smartphones and tablets. This new ... , best-in-class touch controller IP and systems ... in the company,s Japan Design Center. TDDI enables ...
(Date:7/9/2015)... 2015 Research and ... the "Biometrics for Banking; Market & Technology ... to their offering. The adoption for ... growth and the forecast is that by 2020 ... companies involved in delivering biometric systems to the ...
(Date:7/8/2015)... Summary Pancreatic cancer is the 12th ... with a mortality rate of 10.9 deaths per 100,000 ... patients has highlighted a significant need for new and ... by the current market. A highly active pancreatic ... molecule types and mechanisms of action, which provides a ...
Breaking Biology News(10 mins):Synaptics Announces Sampling of Second Generation TDDI Solutions 2Synaptics Announces Sampling of Second Generation TDDI Solutions 3Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 2Global Banking Biometrics Market 2015-2020 - Market & Technology Analysis, Adoption Strategies and Forecasts 3Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3
... scientists have developed a technique to create 3-D images that ... mathematician. This technique uses anisotropic triangles triangles with ... to create 3-D "mesh" computer graphics of more accurate approximations ... shorter amount of time than current techniques. These types of ...
... MOUNT VERNON, WA--In the mild coastal climate of western ... of growing popular fresh-market vegetables in high tunnel production ... vegetable crops, including protection from environmental stresses such as ... obvious benefits, the use of high tunnels for growing ...
... 19, 2013  Organovo Holdings, Inc. (NYSE MKT: ONVO) (" Organovo ... bioprinting technology, has been invited to present at the 25th ... New York December 3-4, 2013. ... Murphy , will be presenting on Wednesday, December 4, at ...
Cached Biology News:UT Dallas computer scientists create 3-D technique 2High tunnel, open-field production systems compared for lettuce, tomato 2Organovo to Present at the 25th Annual Piper Jaffray Healthcare Conference 2
(Date:7/31/2015)... ... ... R-Biopharm is proud to announce the RIDASCREEN® Gliadin Competitive ... been accepted by AOAC International as Official First Action method. The AOAC Official ... R5 Competitive ELISA,” based on a specific monoclonal antibody to potentially celiac toxic ...
(Date:7/30/2015)... ... 30, 2015 , ... The 2015 Market Research Report on the Global ... the global Propanol market with a focus on the Chinese situation. Major companies included ... Oxea, ISU Chemical, Sanofi, Royal Dutch Shell, LG Chem and Zhejiang Xinhua Chemical. , ...
(Date:7/30/2015)...  With over 60,000 customers across the globe, ISN improves the efficiency and effectiveness ... more than 60,000 safe and reliable contractors in over 80 countries. ... ... Over 60,000 Worldwide Customers In ... Headquartered in Dallas, TX , ISN has additional ...
(Date:7/30/2015)... Bedford, NH (PRWEB) , ... July 30, 2015 ... ... efficiency homogenizer for advanced fluid applications and designed for continuous operation up to ... clean-in-place and sterilization-in-place, ensuring safety, efficiency, and preventing toxic contamination. , The Pony™ ...
Breaking Biology Technology:RIDASCREEN® Competitve Gliadin Receives AOAC Official First Action Status 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 2Global Propanol Market Technical Data and Manufacturing Analysis 2020 3ISN Reaches Over 60,000 Worldwide Customers 2ISN Reaches Over 60,000 Worldwide Customers 3GEA Announces the Pony™ NS2006L… a Self-contained, High Pressure Lab Homogenizer for Product Development Ensuring Maximum Efficiency and Safety 2
... 27, 2011 Generex Biotechnology Corporation (OTCBB: GNBT) ( ... out of a blog post on Friday, June 24, ... Company,s common stock fell more than 20% and more ... http://photos.prnewswire.com/prnh/20110106/NY25057LOGO-b) The author of the blog post ...
... subsidiary of Eisai Inc., announced today that it has successfully ... its employees, more than 5 times as much as it ... number equates to over 40 pounds of food donated per ... through collaboration with Philabundance, the area,s largest food bank and ...
... CRANBURY, N.J., June 27, 2011 Amicus Therapeutics (Nasdaq: ... forefront of developing therapies for rare diseases, today announced ... Russell 3000® and small-cap Russell 2000® Indexes. The Company ... Friday, June 24, 2011, when Russell Investments reconstituted its ...
Cached Biology Technology:Generex Provides Clarification of Facts 2Generex Provides Clarification of Facts 3Generex Provides Clarification of Facts 4Generex Provides Clarification of Facts 5Morphotek, Inc. Employees Collect Nearly 9,000 Pounds of Food for Local Families 2Morphotek, Inc. Employees Collect Nearly 9,000 Pounds of Food for Local Families 3Amicus Therapeutics Joins Russell 3000® Index 2
Practical tool for easy, effective sealing of 0.2ml PCR tubes and plates using cap strips....
Caliper offers a wide array of standard LabChip products designed to be used in combination with our AMS 90 and LabChip 3000 microfluidic platforms....
... Time- and temperature-controlled microwave ... Rehydration and Antigen Retrieval of ... offers ease of use, high ... and high performance. When used ...
... discovery system miniaturizes, integrates and automates ... LabChip technology, the LabChip 3000 performs ... flow fashion. LabChip assays are separations-based, ... what is achievable in homogenous, well-based ...
Biology Products: