Navigation Links
Anti-tumor drugs tested by microfluidic device
Date:10/5/2010

Washington, D.C. (October 5, 2010) -- A prototype device developed in Hong Kong will allow laboratory researchers to non-invasively test drugs for their ability to kill tumors by subjecting cancerous cells with different concentration gradients. The new device is built upon microfluidics -- a set of technologies that allows the control and manipulation of fluids at the sub-millimeter scale -- and is described in the American Institute of Physics' journal Biomicrofluidics.

Microfluidic valves within the device, said Hongkai Wu of Hong Kong University of Science and Technology, accurately meter different solutions and mix them to form a stepwise succession of gradients. Then assays measuring cell apoptosis are applied. The device integrates a previously validated analysis method that quantifies the apoptotic process at the level of single cells in real-time.

For this test, researchers measured the activity of the drug etoposide in HeLa cells. Etoposide is a commercially available anticancer compound commonly used in chemotherapy. HeLa cells, derived from human cervical cancer cells, are a line of cells frequently used in research.

The device allows researchers to study the cytotoxicity of multiple concentrations of a drug in parallel on one chip, saving both time and labor and reducing errors caused by variations in conditions often found in larger-scale testing. Also, microfluidic chambers within the device allow long-term tracking of individual cells through fluorescent microscopic imaging that offers high optical sensitivity.

With some other in vitro tests, like DNA analysis, cells need to be killed in order to be studied. In this analysis method, a change in fluorescence occurs when caspase-3, an indicator of cell apoptosis, is activated. In Wu's test, increasing concentrations of etoposide demonstrated correspondingly higher activation of caspase-3. In a control-group chip without etoposide, caspase-3 was not activated.

Wu also said that, "Unlike conventional methods, the microfluidic device permits quantitative data to be obtained from individual cells; the device requires fewer numbers of cells and allows testing of amounts of reagents reduced by about 4 orders of magnitude. In a conventional 96-well plate study, each well is around 1 centimeter, while in our microchip device, each cell chamber has a dimension of hundreds of microns. Reducing the sample size has significant merit because in most cases, the biomolecules in use are extremely expensive, even in quantities in milligrams or less."

While this report concerns a test of five ladder channels on a single chip, higher throughputs should be possible, Wu stated. "This prototype system should be useful in the areas of biology and bioengineering, especially for discovering apoptosis-inducing agents," Wu concluded.


'/>"/>

Contact: Jason Bardi
jbardi@aip.org
301-209-3091
American Institute of Physics
Source:Eurekalert

Related biology news :

1. Researchers identify new anti-tumor gene
2. Childrens National researchers develop novel anti-tumor vaccine
3. Iowa State, Ames Lab researchers identify structure that allows bacteria to resist drugs
4. White House policy advisor talks about legalized drugs
5. Ingredient in soap points toward new drugs for infection that affects 2 billion
6. Case Western Reserve researcher discovers new anti-pathogenic drugs to treat MRSA
7. Scripps scientists develop test providing new pathway for identifying obesity, diabetes drugs
8. U of M researchers identify 2 FDA approved drugs that may fight HIV
9. Scientists reveal new targets for anti-angiogenesis drugs
10. Drugs encased in nanoparticles travel to tumors on the surface of immune-system cells
11. Molecules delivering drugs as they walk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:4/3/2017)... , April 3, 2017  Data ... precision engineering platform, detected a statistically significant ... product prior to treatment and objective response ... the potential to predict whether cancer patients ... to treatment, as well as to improve ...
(Date:3/30/2017)... The research team of The Hong Kong Polytechnic University (PolyU) ... ground breaking 3D fingerprint minutiae recovery and matching technology, pushing contactless ... use in identification, crime investigation, immigration control, security of access and ... ... A research team led by Dr Ajay Kumar ...
(Date:3/29/2017)... 2017  higi, the health IT company that operates ... America , today announced a Series B investment ... EveryMove. The new investment and acquisition accelerates higi,s strategy ... transform population health activities through the collection and workflow ... higi collects and secures data today on behalf of ...
Breaking Biology News(10 mins):
(Date:4/28/2017)... ... April 28, 2017 , ... ... (CRO) has validated a 0.2 ng/mL lower limit (LLOQ) assay for nicotine and ... ng/mL LLOQ assay, the ultra-low trace nicotine assay meets additional needs of tobacco ...
(Date:4/27/2017)... ... April 27, 2017 , ... This month ... (eConsent) solution that simplifies research studies, accelerates study startup, and improves participant engagement. ... Consent™ is the first and only IRB-integrated eConsent solution . , “Our ...
(Date:4/27/2017)... Washington, USA (PRWEB) , ... April 27, 2017 ... ... for optics and photonics , joined other scientists, researchers, engineers, and industry professionals ... measures to strengthen America's ability to compete in the world photonics industry. , ...
(Date:4/26/2017)... Myrtle Beach, SC (PRWEB) , ... April 26, ... ... for the mind, has teamed up with NASA to showcase the future of ... NASA’s Space Launch System (SLS) rocket and Orion spacecraft and includes a guest ...
Breaking Biology Technology: