Navigation Links
Anti-tumor drugs tested by microfluidic device
Date:10/5/2010

Washington, D.C. (October 5, 2010) -- A prototype device developed in Hong Kong will allow laboratory researchers to non-invasively test drugs for their ability to kill tumors by subjecting cancerous cells with different concentration gradients. The new device is built upon microfluidics -- a set of technologies that allows the control and manipulation of fluids at the sub-millimeter scale -- and is described in the American Institute of Physics' journal Biomicrofluidics.

Microfluidic valves within the device, said Hongkai Wu of Hong Kong University of Science and Technology, accurately meter different solutions and mix them to form a stepwise succession of gradients. Then assays measuring cell apoptosis are applied. The device integrates a previously validated analysis method that quantifies the apoptotic process at the level of single cells in real-time.

For this test, researchers measured the activity of the drug etoposide in HeLa cells. Etoposide is a commercially available anticancer compound commonly used in chemotherapy. HeLa cells, derived from human cervical cancer cells, are a line of cells frequently used in research.

The device allows researchers to study the cytotoxicity of multiple concentrations of a drug in parallel on one chip, saving both time and labor and reducing errors caused by variations in conditions often found in larger-scale testing. Also, microfluidic chambers within the device allow long-term tracking of individual cells through fluorescent microscopic imaging that offers high optical sensitivity.

With some other in vitro tests, like DNA analysis, cells need to be killed in order to be studied. In this analysis method, a change in fluorescence occurs when caspase-3, an indicator of cell apoptosis, is activated. In Wu's test, increasing concentrations of etoposide demonstrated correspondingly higher activation of caspase-3. In a control-group chip without etoposide, caspase-3 was not activ
'/>"/>

Contact: Jason Bardi
jbardi@aip.org
301-209-3091
American Institute of Physics
Source:Eurekalert

Page: 1 2

Related biology news :

1. Researchers identify new anti-tumor gene
2. Childrens National researchers develop novel anti-tumor vaccine
3. Iowa State, Ames Lab researchers identify structure that allows bacteria to resist drugs
4. White House policy advisor talks about legalized drugs
5. Ingredient in soap points toward new drugs for infection that affects 2 billion
6. Case Western Reserve researcher discovers new anti-pathogenic drugs to treat MRSA
7. Scripps scientists develop test providing new pathway for identifying obesity, diabetes drugs
8. U of M researchers identify 2 FDA approved drugs that may fight HIV
9. Scientists reveal new targets for anti-angiogenesis drugs
10. Drugs encased in nanoparticles travel to tumors on the surface of immune-system cells
11. Molecules delivering drugs as they walk
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/22/2014)... to dust mites (and chances are you are), help ... University of Iowa have developed a vaccine that can ... response. In animal tests, the nano-sized vaccine package lowered ... the allergens, according to the paper, published in the ... Journal . One big reason why it works, the ...
(Date:7/22/2014)... Schaeffer Center for Health Policy and Economics have ... increased incarceration rates for schizophrenic individuals. , The ... in mental health actually save money, when other ... plans require an extra approval step before tests ... step called prior authorization is intended ...
(Date:7/22/2014)... probiotic that prevents obesity could be on the ... the gut inhibit weight gain, insulin resistance and ... mice, Vanderbilt University investigators have discovered., "Of course ... said senior investigator Sean Davies, Ph.D., assistant professor ... the negative consequences of obesity in mice, even ...
Breaking Biology News(10 mins):Researchers create vaccine for dust-mite allergies 2Are state Medicaid policies sentencing people with mental illnesses to prison? 2Vanderbilt study shows therapeutic bacteria prevent obesity in mice 2
... or proliferation, of jellyfish have shown a substantial, visible ... stinging waters for tourists, even choked intake lines for ... a perception that the world,s oceans are experiencing increases ... warming and overharvesting of fish. Now, a new ...
... An international survey led by Simon Fraser University,s Gerontology ... Stockholm Environment Institute (SEI) is tracking attitudes about the ... involves a consortium of organizations, will provide a snapshot ... issues as climate change. The survey aims ...
... from severe pulmonary diseases including emphysema, lung cancer or ... best chance for survival. The surgical procedure, however, faces ... and rejection of transplanted tissue by the recipient,s immune ... Lung Center (Principal Investigator for this project) in collaboration ...
Cached Biology News:Global experts question claims about jellyfish populations 2Survey tracks '55+' attitudes about the environment 2Lungs clothed in fresh cells offer new hope for transplant patients 2Lungs clothed in fresh cells offer new hope for transplant patients 3
(Date:7/22/2014)... Hussey Copper , a leader in ... copper announces Pullman Regional Hospital,s conversion of more than ... touch surface, at a nominal cost.  This reasonably priced ... other bacteria within 2 hours of exposure 1 and ... infectious diseases in its facilities. Pullman Regional ...
(Date:7/22/2014)... WA (PRWEB) July 22, 2014 Quorum Review ... new service for large clinical trials called Quick Step™ ... is designed to address the research industry’s need for seamless ... The service is available for studies planning 50 or more ... a combined planned North American site count of 80 or ...
(Date:7/22/2014)... Biofeedback – When a behavioral problem ... medication to best treat it can become a matter ... electroencephalography (EEG) can help predict which medicine will offer ... adverse drug events. , The article “Medication Prediction ... issue of the journal Biofeedback offers evidence that quantitative ...
(Date:7/21/2014)... IL (PRWEB) July 21, 2014 ... and simple programming, the new Cole-Parmer® Digital ... is easy to operate for automated process ... for hands-free dispensing. The batch count function counts ... volume or totalizer tracks amount dispensed across multiple ...
Breaking Biology Technology:Pullman Regional Hospital changes more than 1,100 touch points to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 2Pullman Regional Hospital changes more than 1,100 touch points to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 3Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 2Quorum Review IRB Launches New Quick Step™ Service for Large Clinical Trials 3Analyzing Brain Patterns with EEG Can Help Predict Effective Use of Medication 2Analyzing Brain Patterns with EEG Can Help Predict Effective Use of Medication 3New Easy-View Cole-Parmer Digital Gear Pump Drive Makes Performance Monitoring Effortless 2
... of Wisconsin-Madison , justified or otherwise, is that its ... It has world-class scientists and well-developed mechanisms for turning ... of that commercialization takes place within a half-hour drive ... check the figures for per capita wages, unemployment and ...
... the fourth in a series of articles on developing start-up companies ... Madison, Wis. - In my last column for Stage 3, ... In the meantime, I received a thoughtful response about the ... column, dated November 17, 2006, discussed naming the entity. ...
... David Wittwer as CEO of TDS Telecom , , Madison, ... era of leadership at TDS Telecom , as Dave Wittwer ... the company announced. , ,Wittwer, 45, will become president and CEO ... TDS Metrocom . , ,He will be taking ...
Cached Biology Technology:Entrepreneurship grant will build strong foundation 2Entrepreneurship grant will build strong foundation 3Entrepreneurship grant will build strong foundation 4Early Stage, Step 4: Cautionary trademark tales 2Early Stage, Step 4: Cautionary trademark tales 3
Thermal printer paper is used with the thermal printer for the VersaFluor fluorometry systems. Supplied as 10 rolls....
... extraction kit, 100 preps Storage: The kit is ... longer storage, keep all contents of the kit ... purification kit utilizes a silica-gel based membrane which ... in the presence of specialized binding buffers. Nucleotides, ...
The Econo gradient pump rack is a preassembled rack that is used to hold the components of the Econo gradient pump kits....
... Spacer Leu DAP FLISP Assay with ... Em=530 nm) • Accurately measure serine ... or permeabilization • B-Bridge's FLISP Kits ... serine proteases (FLISPs) to measure chymotrypsin-like ...
Biology Products: