New Rochelle, NY, February 2, 2009Since the cloning of Dolly the Sheep over a decade ago, somatic cell nuclear transfer (SCNT) has been considered a promising way to generate human, patient-specific stem cells for therapeutic applications. The shortage of human donor eggs has led to efforts to substitute animal oocytes. However, a new study published online ahead of print in the Volume 11, Number 2, 2009 issue of Cloning and Stem Cells, a peer-reviewed journal published by Mary Ann Liebert, Inc., demonstrates that animal oocytes lack the capacity to fully reprogram adult human cells. The paper is available free online at www.liebertpub.com/clo
Robert Lanza, M.D. and colleagues from Advanced Cell Technology (Worcester, MA), Wake Forest University School of Medicine (Winston Salem, NC), Reproductive Medicine Associates of New Jersey (Morristown), Fertility Specialists of Houston (Texas), Stem Cell Source (Houston), and the College of Veterinary Medicine and Biomedical Sciences at Texas A&M University (College Station), compared the reprogramming of human cells using oocytes obtained from cows, rabbits, and humans. They report their findings in a paper entitled, "Reprogramming of Human Somatic Cells Using Human and Animal Oocytes."
The ability to reprogram human cells using oocytes would enable the production of patient-specific stem cells that could then be differentiated to become any type of somatic cell and used for cell or tissue repair or placement therapy. This extensive reprogramming requires that the oocyte turn on, or up-regulate a large number of genes in the donor nucleus.
Although previous reports have documented the formation of cloned embryos using both human and animal eggs, to date, there have been no data indicating to what extent the donor human DNA was reprogrammed. Lanza et al. show for the first time that human oocytes have the capacity to change these pattern
|Contact: Julia Chapman|
Mary Ann Liebert, Inc./Genetic Engineering News