Navigation Links
Analysis of Lake Washington microbes shows the power of metagenomic approaches
Date:8/17/2008

y of microbes on Earth. To find species of interest, the researchers sequenced microbial communities from Lake Washington sediment samples, Chistoserdova said, because lake sediment is known to be a site of high methane consumption. However, these sediment samples contained over 5,000 species of microbes performing a complex, interconnected array of biochemical tasks.

To enrich the samples for the microbes of interest, the researchers adapted a technique called stable isotope probing. This is the first time the technique has been used on a microbial community, Chistoserdova said. The researchers used five different single-carbon compounds labeled with a heavy isotope of carbon, and fed each compound to a separate sediment sample. The microbes that could consume the compound incorporated the labeled carbon into their DNA, Chistoserdova said, while organisms that couldn't use the compound did not incorporate the label. The labeled DNA was then separated out and sequenced. In this way, microbial "subsamples" were produced that were highly enriched for organisms that could metabolize methane, methanol, methylated amines, formaldehyde and formate.

The functionally enriched samples contained far fewer microbes than the total sample, Chistoserdova said. The sample that was fed methylated amines was simple enough that the group was able to extract the entire genome of a novel microbe, Methylotenera mobilis, that normally comprises less than half a percent of the community, but appears to be a first responder to methylated amines in the environment. The researchers were able to construct much of M. mobilis' biochemistry, and predict that it is also involved in nitrogen cycling, demonstrating the utility of metagenomic analysis.

The DOE JGI performed the sequencing and assembly of these complex metagenomic data sets. The complexity of the community's sequence samples created new challenges for genome assembly. "It is very important for metagenomic
'/>"/>

Contact: David Gilbert
degilbert@lbl.gov
925-296-5643
DOE/Joint Genome Institute
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. Ginkgo SRMs: Tools for product analysis/quality
2. DNA analysis shows true dispersal of protozoa
3. Interacting protein theory awaits test from new neutron analysis tools
4. Analysis of breast and colon cancer genes finds many areas of differences between tumors
5. Tumor genome analysis unveils new insights into lung cancer
6. Powerful integration of lipid metabolic profiling with gene expression analysis
7. Human clones: New U.N. analysis lays out worlds choices
8. More comprehensive analysis of Klamath River basin needed to aid decision makers
9. Analysis calls for medical device information to better serve patients and doctors
10. Avian origins: new analysis confirms ancient beginnings
11. DOE JGI releases a new version of its metagenome data management and analysis system
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/30/2014)... the way for a breakthrough that may prevent ... to poisonous chemicalsparticularly those in pesticides and chemical ... issue of the journal ChemBioChem outlines ... commonly used in pesticides and warfare agents. The ... Engineering Associate Professor of Chemical and Biological Engineering ...
(Date:7/30/2014)... truffle uses reversible epigenetic processes to regulate its genes, ... - a picture of the genome regulation taking place ... journal Genome Biology and illustrates how the ... ,jumping genes,. The authors say this may shed light ... , Black truffles (Tuber melanosporum), also known as Prigord ...
(Date:7/30/2014)... Red Queen in Lewis Carroll,s Through the Looking ... National University of Singapore improved a 35-year-old ecology ... decades to millions of years. , The new ... incorporates the "Red Queen Effect," an evolutionary hypothesis ... which suggests that organisms must constantly increase their ...
Breaking Biology News(10 mins):Engineering a protein to prevent brain damage from toxic agents 2How black truffles deal with the jumpers in their genome 2Classic Lewis Carroll character inspires new ecological model 2Classic Lewis Carroll character inspires new ecological model 3
... virus previously used as a marker in tracing human ... State. Results of this study also suggest that some ... than previously thought. , "The most genetically divergent human ... researcher at Penn State's Center for Infectious Disease Dynamics. ...
... Baptist Medical Center and colleagues at four other medical ... identify genes that may contribute to early atherosclerosis. ... and early adult life who have a genetic predisposition ... for heart disease and stroke sooner and more aggressively," ...
... In a new twist on the slogan “milk does ... staple as a contrast agent in gastrointestinal imaging exams—with ... the annual meeting of the Radiological Society of North ... bowel distension and enhancement as we see with the ...
Cached Biology News:Viral marker of human migration suspect 2Scientists work to identify genes that contribute to early heart attack risk 2Got inexpensive contrast agent? Milk plays new role in imaging 2
(Date:7/31/2014)... Sales Horizons, a leader in sales ... training course – Mastering the Complex Sale ... Drawn from proven sales training programs used by thousands ... Sale online sales training course is available to every ... contact with customers, like technical support, engineers, clinical support, ...
(Date:7/30/2014)... 2014 A study presented at the ... efficacy of CSL Behring,s C1 Inhibitor (C1-INH) concentrate ... highly sensitized patients. C1-INH is a human protein ... The study shows that post-transplant treatment ... levels of complement components 3 and 4, suggesting ...
(Date:7/30/2014)... 30, 2014 Sales Horizons, a leader ... blended sales training programs for companies engaged in complex ... used by thousands of salespeople over 25 years in ... program can be customized to address the unique sales ... blended sales training program consists of two parts: ...
(Date:7/30/2014)... Vycom announces the addition of Thermax ... meet Factory Mutual (FM) 4910 fire propagation and smoke ... benches, process tools, fume hoods, furniture and cabinetry. These ... semiconductor wafers used in electronic chip manufacturing. , ... with excellent workability and superior aesthetics. It is available ...
Breaking Biology Technology:Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 2Sales Horizons Offers Mastering the Complex Sale - a Comprehensive, Online Sales Skills Training Course 3Study Suggests C1-INH May Aid in Prevention of Antibody-Mediated Rejection Following Kidney Transplant 2Study Suggests C1-INH May Aid in Prevention of Antibody-Mediated Rejection Following Kidney Transplant 3Sales Horizons Launches New Blended Sales Training Programs to Companies Engaged in Complex B2B Sales 2Sales Horizons Launches New Blended Sales Training Programs to Companies Engaged in Complex B2B Sales 3Vycom Announces Flametec® Thermax PVC (FM4910 Listed) For a Variety of Clean Room Applications with Excellent Workability and Aesthetics 2
... of articles on developing start-up companies in the technology or ... - You've started your new company, named it, and perhaps ... need additional funding, what other choices do you have? Before ... , ,Most of us recognize that we must have ...
... fast becoming one of the most sought-after spokesmen on behalf ... chief information officers and others will have plenty of prospective ... staff openings. , ,Tim Jump, who will speak at ... in Milwaukee, is a pre-engineering teacher by trade, and ...
... CDW Corp . has signed a definitive merger agreement ... equity firm Madison Dearborn Partners , which has endorsed ... a conference call with investors, CDW chief executive officer John ... growth strategies, including a planned expansion of the Berbee platform. ...
Cached Biology Technology:Early Stage: Step 11 - Other forms of finance 2Early Stage: Step 11 - Other forms of finance 3Early Stage: Step 11 - Other forms of finance 4Jump starting technology education 2Jump starting technology education 3CDW buyer supports Berbee growth plans 2
Rabbit anti-NFkB p105/p50 (Ser337) Class: Antibody Product Group: Signalling molecules and phospho-specific Antibody...
Anti-Kusabira Orange Monoclonal Antibody Description: 100 g Research Focus: tag Storage: -20C Shipping Temperature: 4C...
CD69/CD3 50 tests...
Mouse monoclonal [CIG 3A11/5] to Cig2 (rating: *****) ( Abpromise for all tested applications)....
Biology Products: