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American Chemical Society’s Weekly PressPac -- May 7, 2008
Date:5/12/2008

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American Chemical Society
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American Chemical Society’s Weekly PressPac -- May 7, 2008
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... Brinkmann /Eppendorf SOP, Standard Operating Procedure for Pipettes , , , ... , , , , , ... , , 2 ... , 3 , ...
... , , , Sample ... To extract RNA from washed and pelleted cultured cells, add 200 , ... Isothiocyanate, 25 mM Sodium Citrate, pH 7.0, 1 M b-Mercaptoethanol) , ... To directly extract RNA from cultured cells growing in monolayer, , ...
... , , , , Note: Genomic DNA ... mechanical forces. Use suitable large-bore pipet tips or equipment when , ... , Place a 1 cm tail sample ... may be stored at 20C. To minimize possible cross-contamination, , ...
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Recommend to use at up to 5000X dilutions for cell culture (e.g. Add 1 ul to 1 ml of culture medium.)...
Prepared in distilled water....
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