Navigation Links
ATP splitting in membrane protein dynamically measured for the first time
Date:7/11/2012

Bochum, 11.7.2012 No. 242

Tracked step for step ATP splitting in membrane protein dynamically measured for the first time RUB researchers report in the Journal of Biological Chemistry

How a transport protein obtains its driving force from the energy storage molecule ATP, has been tracked dynamically by RUB researchers. Using time-resolved infrared spectroscopy, they measured the structural changes in the bacterial membrane protein MsbA and its interaction partner ATP. The researchers led by Prof. Dr. Eckhard Hofmann and Prof. Dr. Klaus Gerwert from the Biophysics Department report on the results in the current issue of the Journal of Biological Chemistry.

Transport proteins are associated with various diseases

ABC transporters are membrane proteins that transport various substances from one side of the cell membrane to the other. The driving force for this is provided by the molecule ATP, a universal energy storage of the cells. ATP has three phosphate groups. If one of these splits off, energy is released. The transporters are of great medical significance as they play a central role in the multi-drug resistance of cancer cells to chemotherapeutic substances and are associated with various inherited diseases like cystic fibrosis. In recent years, researchers have uncovered the 3D structures of several of these transporters at the atomic level. Although the architecture of the nanomachines is known, a detailed understanding of how the splitting of the energy carrier ATP dynamically enables the transport of various substances across biological membranes has so far been lacking.

Protein controls ATP splitting

The Bochum researchers have now dynamically tracked the ATP splitting, called hydrolysis, for the first time in the fat transporter MsbA from the bacterium Escherichia coli. Using fourier transform infrared spectroscopy, they studied the motor domains of MsbA, i.e. the part
'/>"/>

Contact: Prof. Dr. Eckhard Hofmann
eckhard.hofmann@bph.rub.de
49-234-322-4463
Ruhr-University Bochum
Source:Eurekalert

Page: 1 2

Related biology news :

1. Advanced Membrane Technology V Conference
2. Patel recognized with NSF Career Award for computer-modeling research on cell membranes
3. Symposium: Protein-Folding Diseases: Models & Mechanisms
4. TLR1 protein drives immune response to certain food-borne illness in mice
5. The appetite-suppressing effect of proteins explained
6. How a protein meal tells your brain you’re full
7. Strawberries activate protection protein to prevent cardiovascular disease
8. Innate immune system protein provides a new target in war against bacterial infections
9. Single protein promotes resistance to widely used anti-estrogen drugs
10. Fibrous Protein Nanocomposites Conference
11. MARC Travel Awards announced for the 26th Annual Symposium of the Protein Society
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:11/5/2014)... Kelly Redmond has dedicated his career to understanding ... a general audience. , As deputy director and ... at Nevada,s Desert Research Institute, Redmond has more ... application and dissemination of climate data to the ... Meeting in San Francisco on December 15-19, 2014 ...
(Date:11/4/2014)... the right time might actually help boost a species, ... in understanding animal populations, pest control and managing fish ... journal Trends in Ecology and Evolution , a ... kind of positive population effect an overall species experiences ... the size and developmental stage of the creatures that ...
(Date:11/4/2014)... published research study examining only marketing directed at ... food restaurants has found that the majority of ... to such marketing tactics. , Authored by Arizona ... the study is the first to examine the ... exterior of fast food restaurants and its relationship ...
Breaking Biology News(10 mins):Nevada climatologist to be honored at AGU Fall Meeting for lifetime of public outreach 2When less is more: Death in moderation boosts population density in nature 2Fast food marketing for children disproportionately affects certain communities 2Fast food marketing for children disproportionately affects certain communities 3
... pollution involving soot that spreads from China over large ... fostering global warming comes from city traffic and ... with coal briquettes. That,s the conclusion of a study ... which resolves long-standing questions about sources of air pollution ...
... On the Monday following a big football game, fans ... saturated fats and sugars, whereas supporters of the winning ... published in Psychological Science , a journal of ... had shown that sport outcomes influence reckless driving, heart ...
... likely to toss a dented can or a chopped-up piece ... to a new study in the Journal of Consumer ... have changed shape are still recyclable, the likelihood of a ... can be determined by the extent to which it has ...
Cached Biology News:For disappointed sports fans, defeats increase consumption of fat and sugar 2Landfill nation: What makes consumers less likely to recycle? 2
(Date:11/18/2014)... Array Architects is proud of a ... the nation's hospitals and health systems. It is a ... their careers to the advancement of both the healthcare ... respective field, Laura Silvoy has been chosen to share ... Simulation Conference. , Laura will present her Process ...
(Date:11/18/2014)... , Nov. 18, 2014  Great Basin Scientific, Inc. (NASDAQ: ... that the management will host a conference call and webcast ... programs on Thursday, Nov. 20, at 4:30 pm EST time. ... made since the Company,s recent IPO and would like to ... management," said Ryan Ashton , President and Chief Executive ...
(Date:11/17/2014)... Soligenix, Inc. (OTCQB: SNGX) (Soligenix or ... that address unmet medical needs in the areas ... formation of a Medical Advisory Board (MAB) to ... it advances the Phase 3 clinical development of ... T-cell lymphoma (CTCL), a rare class of non-Hodgkin,s ...
(Date:11/15/2014)... Collaborative Consulting, a privately held, employee-based professional ... the Boston Globe’s “Top Places to Work 2014”. ... to receive the honor. , The Boston Globe’s ‘Tops ... their most valuable resource: the people who work for ... based on an array of criteria, including the caliber ...
Breaking Biology Technology:Array Healthcare Simulation Expert Slated to Speak at Winter Simulation Conference 2Great Basin Scientific Announces Conference Call to Provide Update on Corporate Progress 2Soligenix Announces Formation of Cutaneous T-Cell Lymphoma Medical Advisory Board 2Soligenix Announces Formation of Cutaneous T-Cell Lymphoma Medical Advisory Board 3Soligenix Announces Formation of Cutaneous T-Cell Lymphoma Medical Advisory Board 4Soligenix Announces Formation of Cutaneous T-Cell Lymphoma Medical Advisory Board 5Soligenix Announces Formation of Cutaneous T-Cell Lymphoma Medical Advisory Board 6Collaborative Consulting Honored with Boston Globe Top Places to Work 2014 2
... appointed FBI Supervising Special Agent (Ret.) Dr. Philip Hayden as its Vice ... ... Wire EON) August 18, 2008 -- Dr. Hayden comes to 4C ... Philip Hayden has spent years involved in many aspects of law enforcement ...
... UNION CITY, Calif. and SHANGHAI, China, ... a privately-held biopharmaceutical company,developing next-generation antibiotics, ... as its first preclinical development candidate. ... resistant gram-positive,bacteria, including methicillin-resistant Staphylococcus aureus ...
... Aureus Pharma, a leading provider of,knowledge ... the release today of the new software ... rapidly evaluate target, cell and,drug profiles using ... in,Aureus Pharma,s AurSCOPE Target Knowledge databases. AurPROFILER ...
Cached Biology Technology: 4C Controls Announces the Appointment of Dr. Philip Hayden as Vice President, Security Assessment & Threat Analysis 2 4C Controls Announces the Appointment of Dr. Philip Hayden as Vice President, Security Assessment & Threat Analysis 3 4C Controls Announces the Appointment of Dr. Philip Hayden as Vice President, Security Assessment & Threat Analysis 4MicuRx Pharmaceuticals Selects Next-Generation Antibiotic Candidate Targeting MRSA and Expands Operation in China 2Aureus Pharma Releases AurPROFILER(R) - a New and Powerful Pharmacology Profiling Solution 2
... with CyScribe GFX Purification Kit, 24 ... of Cy3- and Cy5-labeled cDNA.Optimized reagents ... labeling and purification.Flexible and optimized protocols ... reactions with either Cy3- or Cy5-labeled ...
NORMAL DONOR DONKEY SERUM...
... Supply, 1. 300 V, 400 mA, ... current modes.Single-unit increments in settings and ... submarine, mini vertical, and standard vertical ... and mini tank blotting applications. Category: ...
B3-1...
Biology Products: