Navigation Links
ATP splitting in membrane protein dynamically measured for the first time
Date:7/11/2012

Bochum, 11.7.2012 No. 242

Tracked step for step ATP splitting in membrane protein dynamically measured for the first time RUB researchers report in the Journal of Biological Chemistry

How a transport protein obtains its driving force from the energy storage molecule ATP, has been tracked dynamically by RUB researchers. Using time-resolved infrared spectroscopy, they measured the structural changes in the bacterial membrane protein MsbA and its interaction partner ATP. The researchers led by Prof. Dr. Eckhard Hofmann and Prof. Dr. Klaus Gerwert from the Biophysics Department report on the results in the current issue of the Journal of Biological Chemistry.

Transport proteins are associated with various diseases

ABC transporters are membrane proteins that transport various substances from one side of the cell membrane to the other. The driving force for this is provided by the molecule ATP, a universal energy storage of the cells. ATP has three phosphate groups. If one of these splits off, energy is released. The transporters are of great medical significance as they play a central role in the multi-drug resistance of cancer cells to chemotherapeutic substances and are associated with various inherited diseases like cystic fibrosis. In recent years, researchers have uncovered the 3D structures of several of these transporters at the atomic level. Although the architecture of the nanomachines is known, a detailed understanding of how the splitting of the energy carrier ATP dynamically enables the transport of various substances across biological membranes has so far been lacking.

Protein controls ATP splitting

The Bochum researchers have now dynamically tracked the ATP splitting, called hydrolysis, for the first time in the fat transporter MsbA from the bacterium Escherichia coli. Using fourier transform infrared spectroscopy, they studied the motor domains of MsbA, i.e. the part of the protein where the ATP splitting takes place. Using this method, researchers can track minute changes in the protein in the range of nanoseconds. Simultaneously, the method also records changes in the molecules the protein interacts with - in this case ATP.

Phosphate signals reveal what happens during the splitting

The big challenge presented by the data analysis is to assign the signals in the measured spectrum to specific molecules or molecular groups. If this is successful, you can see which groups of molecules are structurally changed and when. The biophysicists marked the phosphate groups of the ATP molecule, so that they left characteristic signals in the spectrum. In this way they tracked, how ATP bound to the transport protein, how one of its three phosphate groups split off and was released into the environment without first latching back on to the protein. "Our data also provides important clues as to how the protein moves during ATP hydrolysis. This lays the foundation for the study of the whole membrane protein, which we are going to tackle next", says Professor Hofmann. The investigations were supported by the Protein Research Department at the RUB and funds of the collaborative research centre SFB 642 "GTP and ATP dependent membrane processes", whose speaker is Prof. Dr. Klaus Gerwert.


'/>"/>
Contact: Prof. Dr. Eckhard Hofmann
eckhard.hofmann@bph.rub.de
49-234-322-4463
Ruhr-University Bochum
Source:Eurekalert

Related biology news :

1. Advanced Membrane Technology V Conference
2. Patel recognized with NSF Career Award for computer-modeling research on cell membranes
3. Symposium: Protein-Folding Diseases: Models & Mechanisms
4. TLR1 protein drives immune response to certain food-borne illness in mice
5. The appetite-suppressing effect of proteins explained
6. How a protein meal tells your brain you’re full
7. Strawberries activate protection protein to prevent cardiovascular disease
8. Innate immune system protein provides a new target in war against bacterial infections
9. Single protein promotes resistance to widely used anti-estrogen drugs
10. Fibrous Protein Nanocomposites Conference
11. MARC Travel Awards announced for the 26th Annual Symposium of the Protein Society
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:7/20/2017)... -- Delta (NYSE: DAL ) customers now can use fingerprints ... Washington National Airport (DCA). ... Delta launches biometrics to board aircraft at Reagan Washington National ... Delta,s biometric boarding pass experience that launched ... into the boarding process to allow eligible Delta SkyMiles Members who are ...
(Date:6/30/2017)... Today, American Trucking Associations announced Seeing ... and eye tracking software, became the newest member ... "Artificial intelligence and advanced sensing algorithms ... driver,s attentiveness levels while on the road.  Drivers ... fatigue and prevent potential accidents, which could lead ...
(Date:6/23/2017)... ITHACA, N.Y. , June 23, 2017 ... a leader in dairy research, today announced a new ... help reduce the chances that the global milk supply ... this dairy project, Cornell University has become the newest ... Food Supply Chain, a food safety initiative that includes ...
Breaking Biology News(10 mins):
(Date:7/28/2017)... ... July 28, 2017 , ... ... provider, announces BiobankPro®, a workflow based enterprise solution for next-generation biobanking. BiobankPro ... freezer centered, BiobankPro® offers a solution that is specimen centered. , “Your ...
(Date:7/27/2017)... ... July 27, 2017 , ... ... a Top 100 Agency award by Medical Marketing & Media magazine ... , According to its website, MM&M “is the media brand of record for ...
(Date:7/27/2017)... , ... July 27, 2017 , ... ... sexual characteristics, but it is also synthesized in female at a much lower ... women with hirsutism, virilization, oligomenorrhea, and possible testosterone deficiency in women. , ...
(Date:7/27/2017)... ... July 27, 2017 , ... Cynvenio Biosystems, Inc. ... today announced that its LiquidBiopsy rare cell and cell-free isolation platform has been ... Seoul, Severance Hospital is one of the oldest and largest university hospitals in ...
Breaking Biology Technology: