Navigation Links
ASU scientists develop universal DNA reader to advance faster, cheaper sequencing efforts
Date:2/11/2010

of the DNA bases. More recently, they demonstrated the ability to thread DNA sequences through the narrow hole of a fundamental building block of nanotechnology, the carbon nanotube.

Lindsay's team relies on the eyes of nanotechnology, scanning tunneling- (STM) and atomic force- (ATM) microscopes, to make their measurements. The microscopes have a delicate electrode tip that is held very close to the DNA sample. In their latest innovation, Lindsay's team made two electrodes, one on the end of microscope probe, and another on the surface, that had their tiny ends chemically modified to attract and catch the DNA between a gap like a pair of chemical tweezers. The gap between these functionalized electrodes had to be adjusted to find the chemical bonding sweet spot, so that when a single chemical base of DNA passed through a tiny, 2.5 nanometer gap between two gold electrodes, it momentarily sticks to the electrodes and a small increase in the current is detected. Any smaller, and the molecules would be able to bind in many configurations, confusing the readout, any bigger and smaller bases would not be detected.

"What we did was to narrow the number of types of bound configurations to just one per DNA base," said Lindsay. "The beauty of the approach is that all the four bases just fit the 2.5 nanometer gap, so it is one size fits all, but only just so!"

At this scale, which is just a few atomic diameters wide, quantum phenomena are at play where the electrons can actually leak from one electrode to the other, tunneling through the DNA bases in the process. Each of the chemical bases of the DNA genetic code, abbreviated A, C, T or G, gives a unique electrical signature as they pass between the gap in the electrodes. By trial and error, and a bit of serendipity, they discovered that just a single chemical modification to both electrodes could distinguish between all 4 DNA bases.

"We've now made a generic DNA sequence reader and
'/>"/>

Contact: Joe Caspermeyer
joseph.caspermeyer@asu.edu
480-727-0369
Arizona State University
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. UK scientists working to help cut ID theft
2. Scientists show that mitochondrial DNA variants are linked to risk factors for type 2 diabetes
3. Comet probes reveal evidence of origin of life, scientists claim
4. Scientists link fragile X tremor/ataxia syndrome to binding protein in RNA
5. Male elephants get photo IDs from scientists
6. Scientists retrace evolution with first atomic structure of an ancient protein
7. Muscle mass: Scientists identify novel mode of transcriptional regulation during myogenesis
8. Carnegie Mellon scientists develop nanogels that enable controlled delivery of carbohydrate drugs
9. Clemson scientists shed light on molecules in living cells
10. Scientists tackle mystery mountain illness
11. T. rex quicker than Becks, say scientists
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
ASU scientists develop universal DNA reader to advance faster, cheaper sequencing efforts
(Date:6/25/2015)... , June 25, 2015  TAKE Solutions Ltd., a ... by the United States Patent and Trademark Office (USPTO) ... process leverages TAKE Solutions, Clinical Accelerators to reduce the ... (when compared to standardization without the accelerators), thus reducing ... At the heart of the patented ...
(Date:6/24/2015)... 24, 2015 Biometry authentication provider KeyLemon ... password solution one face in. ... one face in, entering the expanding biometric authentication ... multifactor-authentication biometric answer to the password problem. With ... partnership with KeyLemon, one face in allows users ...
(Date:6/18/2015)... This report analyzes the worldwide markets for ... Segments, which includes Immunoassay Analyzers and Reagents: Enzyme Immunoassay ... Systems, and Nephelometric Immunoassay Systems. The report provides separate ... , Japan , Europe ... Latin America , and Rest of World. Annual ...
Breaking Biology News(10 mins):TAKE Solutions Awarded Patent By USPTO 2KeyLemon Announces Partnership With Biometric Password Solution onefacein 2Global Immunoassay Systems Market 2Global Immunoassay Systems Market 3Global Immunoassay Systems Market 4Global Immunoassay Systems Market 5Global Immunoassay Systems Market 6Global Immunoassay Systems Market 7Global Immunoassay Systems Market 8Global Immunoassay Systems Market 9Global Immunoassay Systems Market 10Global Immunoassay Systems Market 11Global Immunoassay Systems Market 12Global Immunoassay Systems Market 13Global Immunoassay Systems Market 14Global Immunoassay Systems Market 15Global Immunoassay Systems Market 16Global Immunoassay Systems Market 17Global Immunoassay Systems Market 18Global Immunoassay Systems Market 19Global Immunoassay Systems Market 20Global Immunoassay Systems Market 21Global Immunoassay Systems Market 22Global Immunoassay Systems Market 23Global Immunoassay Systems Market 24Global Immunoassay Systems Market 25Global Immunoassay Systems Market 26Global Immunoassay Systems Market 27Global Immunoassay Systems Market 28Global Immunoassay Systems Market 29
... people with the genetic condition known as phenylketonuria (PKU), diet ... and instead get their daily dose of this key macronutrient ... it they must; deviating from this strict dietary regimen puts ... In the near future, fortunately, a better option may become ...
... are at increased risk for type 2 diabetes, Medical ... study of 50 people newly-diagnosed with schizophrenia or a ... 16 percent had either diabetes or an abnormal rate ... of the MCG Department of Psychiatry and Health Behavior. ...
... CRUZ, CA--A Cancer Genomics Browser developed by researchers at ... way to visualize and analyze data from studies aimed ... roots of the disease. The browser consists ... researchers find patterns in the huge amounts of clinical ...
Cached Biology News:UW-Madison study reveals new options for people with PKU 2UW-Madison study reveals new options for people with PKU 3Researchers discover link between schizophrenia and diabetes 2Cancer Genomics Browser gives cancer researchers a powerful new tool 2Cancer Genomics Browser gives cancer researchers a powerful new tool 3Cancer Genomics Browser gives cancer researchers a powerful new tool 4
(Date:7/6/2015)... July 6, 2015  Emdeon Inc. today ... definitive agreement to acquire Altegra Health, a ... that combine data aggregation and analytics with ... achieve actionable insights and improved management for ... Health,s risk adjustment and quality analytics and ...
(Date:7/6/2015)... , July 6, 2015 ImmunoCellular Therapeutics, Ltd. ... an agreement with Novella Clinical (Novella), to ... patients with newly diagnosed glioblastoma. Novella is a full-service, ... to small to mid-sized oncology companies. The ICT-107 phase ... the US, Europe and ...
(Date:7/3/2015)... ... ... The global failure analysis market is expected to reach $7,147.0 million by 2020, at ... grow at the highest CAGR of 8.46%; its growth is expected to be driven ... institutions for root cause analysis of failure. The market based on the geographic regions ...
(Date:7/2/2015)... ... ... have been used in countless labs for decades. In the past, people had to ... expected, this was a daunting and tiring process looking at endless data tables and charts. ... navigate the complex catalog structure. , Now thanks to FireflySci, scientists are throwing away those ...
Breaking Biology Technology:Emdeon to Acquire Altegra Health 2Emdeon to Acquire Altegra Health 3Emdeon to Acquire Altegra Health 4ImmunoCellular Therapeutics Establishes Agreement with Novella Clinical to Conduct ICT-107 Phase 3 Registration Trial in Glioblastoma 2Failure Analysis Market to Grow at 7.52% CAGR to 2020 2Failure Analysis Market to Grow at 7.52% CAGR to 2020 3Failure Analysis Market to Grow at 7.52% CAGR to 2020 4Failure Analysis Market to Grow at 7.52% CAGR to 2020 5New Cuvette E-Commerce Website Launched by FireflySci 2
... Ahmed Fazil Madison, Wis. - The 2006 ... has launched its first identity theft product, and has ... and three million customers after two years. , ,This probably ... plans to reach positive cash flow, but consider the total ...
... of Fresno in California's San Joaquin Valley is one of ... from grapes to livestock, from almonds to dairy products, and ... San Joaquin Valley these days is biofuel. , ,Like many ... recent visit are happy that demand for ethanol and biodiesel ...
... appears that Wisconsin has failed in its bid to lure a ... a maker of neural stem cell kits for researchers, apparently has ... it was spun out of the University of Georgia . ... likely that the company will remain in Georgia, despite an incentive ...
Cached Biology Technology:Identity theft start up has fast-track growth plans 2Identity theft start up has fast-track growth plans 3Kind approach to leveraging biofuels 2Kind approach to leveraging biofuels 3Georgia stem cell company likely to stay put 2
... ester can be used to ... The covalently bound acridinium NHS ... the presence of hydrogen peroxide. ... be used as a detection ...
... up to 6 strain chambers with precise uniaxial ... design ensures that cells grow over long periods ... This unit allows you to control the frequency ... unique strain settings. The instrument fits in a ...
... a novel procedure for the uniform amplification ... genome DNA from small samples. This method ... and highly reproducible amplification and labeling procedure ... REPLI-g Mini Kit module (QIAGEN) and a ...
... using PULSin may be superior to transfection ... traditional transfection, with PULSin you can study ... time course of proteins in cells. Likewise, ... information than traditional RNA interference experiments. ...
Biology Products: