Navigation Links
ASU researchers develop new device to help image key proteins at room temperature
Date:12/20/2013

zing the sample to avoid damage, and so it reveals the structure which actually occurs in a cell at room temperature, not the frozen structure," Spence added. "The 50 femtosecond pulses (120 per second) 'outrun' radiation damage, giving a clear picture of the structure before it is vaporized by the beam."

The femtosecond crystallography technique could enable researchers to view molecular dynamics at a time-scale never observed before. Spence said the method basically operates by collecting the scattering for the image so quickly that images are obtained before the sample is destroyed by the x-ray beam.

By 'outrunning' radiation-damage processes in this way, the researchers can record the time-evolution of molecular processes at room temperature, he said.

Spence said ASU played a crucial role in the project described in Science, through the invention by Uwe Weierstall (an ASU physics professor) of an entirely new device for sample delivery suited to this class of proteins.

The lipic cubic phase (LCP) injector that Weierstall developed replaces the continuous stream of liquid (which sends a continuously refreshed stream of proteins across the pulsed x-ray beam) with a slowly moving viscous stream of 'lipid cubic phase solution,' which has the consistency of automobile grease.

"We call it our 'toothpaste jet,'" Spence said.

He added that the LCP solves three problems associated with previous SFX work, and made this new work possible:

  • The viscosity slows the flow rate so the crystals emerge at about the same rate as the x-ray pulses come along, hence no protein is wasted. This is important for the study of human protein, which is more costly than diamond on a per gram basis.
  • The "hit rate" is very high. Nearly all x-ray pulses hit protein particles.
  • Most important, LCP is itself a growth medium for protein nanocrystals.

"A big problem with the SFX work we have been
'/>"/>

Contact: Skip Derra
skip.derra@asu.edu
480-965-4823
Arizona State University
Source:Eurekalert

Page: 1 2 3

Related biology news :

1. With sinus study, Saint Louis University researchers find that harmless members of microbiome spark immune reaction
2. New anti-HIV drug target identified by University of Minnesota researchers
3. MU researchers develop advanced 3-dimensional force microscope
4. Researchers explain why some wound infections become chronic
5. Stanford researchers take a step toward developing a universal flu vaccine
6. Researchers engineer a hybrid 5 times more effective in delivering genetic material into cells
7. New organization brings together top researchers to sequence genomes of invertebrates
8. Bonefish spawning behavior in the Bahamas surprises researchers, should aid conservation
9. Where water is limited, researchers determine how much water is enough
10. Researchers at Penn help develop a dynamic model of tissue failure
11. Leibniz Prizes 2014: DFG honors 11 outstanding researchers
Post Your Comments:
*Name:
*Comment:
*Email:
(Date:9/15/2014)... lowland tapir populations may continue to drop in French ... the Office National de la Chasse et de la ... Global reviewed data retrieved from camera traps in the ... compared this data to current harvest rates in the ... in French Guiana," said Matthias Tobler, a scientist with ...
(Date:9/15/2014)... INDIANAPOLIS -- Adding a common epilepsy drug to a ... fewer side effects. Moreover, the combination can reduce the ... to a team of pain researchers at Indiana University. ... patients with neuropathic pain, a difficult-to-treat condition often felt ... tissue damage. , "There is a huge unmet need ...
(Date:9/15/2014)... a vital source of energy for both plants and ... way into the cell could lead to the design ... in the amount of fruits and vegetables farmers are ... one of these "pathways" into the cell by piecing ... strand of spider silk. , To determine the size, ...
Breaking Biology News(10 mins):IU study: Combining epilepsy drug, morphine can result in less pain, lower opioid doses 2X-rays unlock a protein's SWEET side 2X-rays unlock a protein's SWEET side 3
... diabetes during pregnancy is directly controlled by serotonin, a ... a neurotransmitter, and is influenced by the amount of ... to new findings of an international team led by ... to simple dietary solutions and possible therapeutics for the ...
... rich? Smithsonian researchers have new evidence that the answer ... to a study published in the journal, Nature ., ... not grow and survive well under their mothers or other ... fellow at the University of WisconsinMilwaukee and the Smithsonian Tropical ...
... available in Spanish . , At a time ... Service (ARS)-funded scientists have contributed to a limited but growing ... cognitive function. Cognitive function is measured by the ... use available information for activities of daily life. Alzheimer,s disease, ...
Cached Biology News:Gestational diabetes linked to serotonin and dietary protein 2Gestational diabetes linked to serotonin and dietary protein 3Biodiversity's holy grail is in the soil 2Vitamin D and mental agility in elders 2
(Date:9/11/2014)... stem cells (hiPSCs), researchers have gained new insight ... altered patterns of neuronal signaling associated with this ... from the hiPSCs of healthy individuals and of ... these stem cells to release neurotransmitters, such as ... are linked to various disorders. By discovering a ...
(Date:9/11/2014)... at Chalmers University of Technology are first to show ... atom. They can thereby demonstrate phenomena from quantum physics ... results will be published in the journal Science ... well known and has been studied extensively in the ... kind of interaction with sound waves has been a ...
(Date:9/11/2014)... paper published this week in Science , the ... Manchester,s Nobel laureate Sir Andre Geim report a material ... applied fields, similar to sailboats driven diagonally to the ... chicken wire made from carbon but with a ... state by placing it on top of boron nitride, ...
(Date:9/11/2014)... Fla. , Sept. 11, 2014 /PRNewswire/ ... anti-infective pharmaceuticals, announced today that the United ... a ,Notice of Allowance, for patent application ... Uses,.  The ,486 patent application relates to ... through gene-silencing and can be designed to ...
Breaking Biology Technology:Stem cells help researchers understand how schizophrenic brains function 2The sound of an atom has been captured 2New species of electrons can lead to better computing 2Lakewood-Amedex Receives Notice Of Allowance From The United States Patent and Trademark Office (USPTO) For A New Patent Covering Its nanoRNA Gene-Silencing Technology 2Lakewood-Amedex Receives Notice Of Allowance From The United States Patent and Trademark Office (USPTO) For A New Patent Covering Its nanoRNA Gene-Silencing Technology 3
... Sector, TROY, Mich., Nov. 10 Automation ... data from its,Fourth Annual Technology Industry Report: Driving ... on Wednesday, Nov. 12 at the,Troy Marriott. The ... regarding Automation Alley,s rapidly growing presence in the,life ...
... -- China Medicine,Corporation (OTC Bulletin Board: CHME) ... and distributor of prescription and over the ... supplements, and medical devices and medical formulations, ... call at 9:00 a.m. Eastern Time,on Thursday, ...
... The West Michigan Science,& Technology Initiative and ... a new medical device company, TransCorp, Inc. TransCorp ... Technology offices in,Grand Valley State University,s Cook-DeVos Center ... and commercialize novel surgical instruments,and implants. Other participants ...
Cached Biology Technology:Automation Alley Releases Report on State of Southeast Michigan's Technology Economy 2Automation Alley Releases Report on State of Southeast Michigan's Technology Economy 3China Medicine Announces Conference Call to Discuss Third Quarter 2008 Results 2West Michigan's Newest Medical Device Company Receives Support from the West Michigan Science & Technology Initiative and EDF Ventures 2
RayBio Human Chemokine Antibody Array 1.1 (8) with Accessory, detects 38 chemokine factors (for lysate) Class: Antibody Array Products Product Group: Antibody Array...
... The CultureWell cell culture chambered ... cultured cells for staining and ... medical-grade silicone gaskets with standard ... sterile and ready to use. ...
Adhesive seal-tab, for HybriWell™ hybridization sealing system *set of 400*...
...
Biology Products: