Navigation Links
A single cell endoscope
Date:12/20/2011

Yang and Luke Lee.

Despite significant advancements in electron and scanning probe microscopy, visible light microscopy remains the workhorse for the study of biological cells. Because cells are optically transparent, they can be noninvasively imaged with visible light in three-dimensions. Also, visible light allows the fluorescent tagging and detection of cellular constituents, such as proteins, nucleic acids and lipids. The one drawback to visible light imaging in biology has been the diffraction barrier, which prevents visible light from resolving structures smaller than half the wavelength of the incident light. Recent breakthroughs in nanophotonics have made it possible to overcome this barrier and bring subcellular components into view with optical imaging systems. However, such systems are complex, expensive and, oddly enough, bulky in size.

"Previously, we had shown that subwavelength dielectric nanowire waveguides can efficiently shuttle ultraviolet and visible light in air and fluidic media," Yang says. "By incorporating one of our nanophotonic components into a simple, low-cost, bench-top fibre-optical set-up, we were able to miniaturize our endoscopic system."

To test their nanowire endoscope as a local light source for subcellular imaging, Yang and his co-authors optically coupled it to an excitation laser then waveguided blue light across the membrane and into the interiors of individual HeLa cells, the most commonly used immortalized human cell line for scientific research.

"The optical output from the endoscope emission was closely confined to the nanowire tip and thereby offered highly directional and localized illumination," Yang says. "The insertion of our tin oxide nanowire into the cell cytoplasm did not induce cell death, apoptosis, significant cellular stress, or membrane rupture. Moreover, illuminating the intracellular environment of HeLa cells with blue light using the nanoprobe did not harm
'/>"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Evolution at warp speed: Hatcheries change salmon genetics after a single generation
2. New Columbia engineering technique diagnoses non-periodic arrhythmias in a single heartbeat
3. Smoking a single cigarette may have immediate effect on young adults
4. Single protein, key to ebola virus infection, could aid in drug design
5. Single, key gene discovery could streamline production of biofuels
6. Rapid evolution within single crop-growing season increases insect pest numbers
7. Single gene controls development of many forms of polycystic disease
8. Single GFP-expressing cell is basis of living laser device
9. Improving health assessments with a single cell
10. Scientists observe single gene activity in living cells
11. Species unique to single island should not be rare there
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A single cell endoscope
(Date:2/5/2015)... 28, 2015 New Market Research ... Segment Forecasts To 2020 has Been Added to GrandViewReseach.com ... is expected to reach USD 5.10 billion by 2020, ... Inc. IR cameras help identify the site of tissue ... witness surging demand in medical imaging applications. They have ...
(Date:2/5/2015)... LA JOLLA, Calif. , Jan. 30, 2015 /PRNewswire/ ... realizes the great power and potential for genomic science ... to aid in disease prevention and treatment.  I was ... a potential new, government-funded precision medicine program. ... on advancing the science of genomics—from the first sequenced ...
(Date:1/22/2015)... , Jan. 9, 2015 Valid S.A. ("Company") (BM&FBOVESPA: VLID3 ... in general the State of Washington,s ... a new enrollment and central issuance system for driver,s licenses ... The project planning and development will start in January ...
Breaking Biology News(10 mins):IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 2IR Camera Market Worth $5.10 Billion By 2020: Grand View Research, Inc. 3J. Craig Venter, Ph.D., Co-Founder and CEO, Human Longevity, Inc. (HLI) Participates in White House Precision Medicine Event 2Valid USA Signs Contract For Washington Driver's License Issuance 2
... University of Toronto (U of T) have found that ... species opts for sexual over asexual reproduction. The ... Aneil Agrawal of the Department of Ecology & Evolutionary ... habitats characterized by uneven concentrations of its own ...
... Inc. (Nasdaq: AWRE ), a leading technology innovator ... a conference call to discuss the company,s operating results for ... 5:00 p.m. Eastern Time. President and CEO Edmund Reiter and ... This call is being webcast by Thomson and can ...
... A team of biomedical engineers from Columbia University, University ... million dollar, five-year Multi University Research Initiative (MURI) grant from ... blast waves on the neural circuitry of the brain. ... improvised explosive devices (IEDs), blast-induced traumatic brain injury (TBI) has ...
Cached Biology News:Aware, Inc. Announces Q3'10 Earnings Conference Call 2Columbia engineer part of team to receive $6.2 million DOD grant for blast research 2
(Date:2/27/2015)... , Feb. 27, 2015 Research and Markets ... "Global Market Report of Trypsin" report to their ... (CAS 9002-07-7) aims at providing comprehensive data on Trypsin globally ... , North America , ... pays close attention to Trypsin This report focuses ...
(Date:2/27/2015)... (PRWEB) February 27, 2015 The ... system (Nexera UC) can sequentially analyze up to ... combined with high-sensitivity detection of targets by mass ... designed to fulfill the measurement requirements of a ... food products, drug delivery and search for disease ...
(Date:2/27/2015)... Feb. 27, 2015  Pfenex Inc. (NYSE MKT: PFNX), ... biosimilar therapeutics, today announced that it will be presenting ... on March 2 nd at 3pm PST. Patrick Lucy, ... panel discussing the current state of the biosimilar industry ... For more information on CALBIO 2015 ...
(Date:2/27/2015)... South San Francisco, CA (PRWEB) February 27, 2015 ... it is joining forces on February 28 with patients ... globally to raise awareness for Rare Disease Day®. ... of rare diseases and calling attention to the special ... support community around them. , “Cytokinetics is proud to ...
Breaking Biology Technology:Global Market Report of Trypsin 2014-2018 2Shimadzu Corporation Launches Nexera UC - The World’s Foremost Unified Chromatography System 2Shimadzu Corporation Launches Nexera UC - The World’s Foremost Unified Chromatography System 3Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 2Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 3Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 4Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 5Cytokinetics Joins Global Movement to Raise Awareness for Rare Diseases 6
... Aug. 14 /Xinhua-PRNewswire-FirstCall/ -- China Sky One,Medical, ... Company,) (Amex: CSY ), a,leading fully ... in the People,s Republic of China ("PRC"), ... spray for the treatment,and prevention of rheumatic ...
... Technology ... ... (Nasdaq: PRXL ), a leading global biopharmaceutical services,organization, today ... leading clinical technology organization. By combining,ClinPhone with Perceptive Informatics, PAREXEL,s ...
... to Host Conference Call at 4:30 p.m. ET Today -, ... ) today reported financial results for its fourth quarter,and fiscal ... fourth quarter of,fiscal 2008 was $4.6 million, or $0.18 per ... per share, for the fourth quarter of fiscal 2007., ...
Cached Biology Technology:China Sky One Medical, Inc. Develops Nasal Spray for Rheumatic Disease 2China Sky One Medical, Inc. Develops Nasal Spray for Rheumatic Disease 3PAREXEL Closes the Acquisition of ClinPhone 2PAREXEL Closes the Acquisition of ClinPhone 3PAREXEL Closes the Acquisition of ClinPhone 4PAREXEL Closes the Acquisition of ClinPhone 5Pharmacyclics Reports Fourth Quarter and Fiscal 2008 Financial Results 2Pharmacyclics Reports Fourth Quarter and Fiscal 2008 Financial Results 3Pharmacyclics Reports Fourth Quarter and Fiscal 2008 Financial Results 4Pharmacyclics Reports Fourth Quarter and Fiscal 2008 Financial Results 5Pharmacyclics Reports Fourth Quarter and Fiscal 2008 Financial Results 6
Polyclonal Antibody to CRTR-1...
Recognizes NSCL1. The molecular weight of the protein is 14,616 Daltons....
Mouse monoclonal [3G131] to Desmoglein 1 ( Abpromise for all tested applications). entrezGeneID: 1828 SwissProtID: Q02413...
Mouse monoclonal [2D7/1] to E.coli LPS ( Abpromise for all tested applications). Antigen: Tissue / cell preparation: Escherichia coli J5 cells....
Biology Products: