Navigation Links
A single atom controls motility required for bacterial infection
Date:1/4/2010

d twitching motility, and without it Pseudomonas, a common cause of hospital-acquired pneumonia would never be able to move from the lung tissue into the bloodstream, where the infection becomes lethal."

The researchers knew that inside the cell lie the tiny little motors called ATPases that drive the extension and retraction of the pili. One of these ATPases is the extension motor, which sticks the bacteria's leg out. The other ATPase is the retraction motor, which pulls it back in. But what wasn't clear was how the two motors were coordinated so that pushing and pulling didn't occur at the same time. That is what Redinbo and Wolfgang set out to discover.

First, they resolved the crystal structure of the Pseudomonas PilY1 protein, which other research had shown was necessary for the creation of pili. They made large amounts of the protein, coaxed it out of solution so that it formed a crystal, and then put the crystal under intense x-ray beams through a process called x-ray diffraction that resulted in a series of spots. Based on the spots, the researchers calculated what the protein looked like. When they studied the structure, one particular site the binding site of a calcium atom looked like it could be important for the function of the protein. So the researchers began to tinker with the site, looking to see if the changes they made affected the protein's behavior.

When they changed the protein so it could no longer bind calcium, the bacteria couldn't make any legs. When they fooled the protein into thinking it was forever bound to calcium, the bacteria made legs but couldn't retract them, essentially becoming paralyzed. The results suggested that the protein has to bind calcium to make legs, but it also has to be able to let go of the calcium to pull the legs back in.

"We found it pretty remarkable that the binding of a single atom to a protein that is outside the cell is sufficient to tell these motors that are inside t
'/>"/>

Contact: Tom Hughes
tahughes@unch.unc.edu
919-966-6047
University of North Carolina School of Medicine
Source:Eurekalert  

Page: 1 2 3

Related biology news :

1. Model for the assembly of advanced, single-molecule-based electronic components developed at Pitt
2. Single-largest biodiversity survey says primary rainforest is irreplaceable
3. New technique captures chemical reactions in a single living cell at unprecedented resolution
4. Gene study supports single main migration across Bering Strait
5. Baumann lab identifies elusive telomere RNA subunit in single cell model
6. Ebola virus disarmed by excising a single gene
7. Blue-eyed humans have a single, common ancestor
8. Rice scientists make breakthrough in single-molecule sensing
9. Single-celled bacterium works 24-7
10. RevGenUK, a single-stop shop for use in functional genomics
11. Researchers observe spontaneous ratcheting of single ribosome molecules
Post Your Comments:
*Name:
*Comment:
*Email:
Related Image:
A single atom controls motility required for bacterial infection
(Date:7/7/2015)... NXT-ID, Inc. (NASDAQ: NXTD ) ("NXT-ID" or the ... market announces a revised version of one of its first 30 ... on CNBC in New York , ... San Francisco metro areas.    , ... to replace all the cards in your wallet and keep your ...
(Date:7/2/2015)... Fingerprint Cards has received an order for its ... distributor World Peace Industrial Group (WPI), part of WPG Holdings. ... . Deliveries are planned to take place during Q3 ... manufacturers in Asia . The order value ... of approximately 2,200 MSEK for 2015. Jörgen Lantto, ...
(Date:6/29/2015)... Research and Markets( http://www.researchandmarkets.com/research/zj7h4f/latin_america ... America Biomedical Sensors Market - Growth, Trends & Forecasts ... Latin America Biomedical Sensors market is estimated at $0.65 ... the period 2014-2020 The near future will ... formulation of each individual. These sensors can be set ...
Breaking Biology News(10 mins):Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 2Wocket Smart Wallet Commercials Airing on CNBC to Showcase This Summer's Must- Have Tech Product 3FPC Receives Order for Touch Fingerprint Sensors of 268 MSEK 2Latin America Biomedical Sensors Market Report 2015-2020 2
... ,Western scrub jays summon others to screech over the body ... University of California, Davis. The birds, cacophonous "funerals" can last ... suggested that other animals, including elephants, chimpanzees and birds in ... Teresa Iglesias, the UC Davis graduate student who carried out ...
... Researchers have created a new type of miniature pump activated ... powered by fermentation., The micropump contains bakers yeast and sugar ... patch is placed on the skin, the body heat and ... generating a small amount of carbon dioxide gas. The gas ...
... reactors has the promise to be safe, sustainable and limitless. ... to the commercial energy market. This is partly because the ... A team of researchers at the University of Tennessee, Knoxville, ... institutions, is trying to change that. Led by Brian ...
Cached Biology News:Scrub jays react to their dead 2Body heat, fermentation drive new drug-delivery 'micropump' 2University of Tennessee, ORNL lead national team to study nuclear fusion reactors 2University of Tennessee, ORNL lead national team to study nuclear fusion reactors 3
(Date:7/27/2015)... The Brain & Behavior Research ... annual Klerman and Freedman Prizes, recognizing exceptional clinical ... supported by NARSAD Young Investigator Grants. The grants ... in neurobiological and psychosocial research, garner pilot data ... detection, treatment, prevention and cure of mental illnesses. ...
(Date:7/25/2015)... ... July 25, 2015 , ... ... series. It precisely checks and adjusts the surface temperature of the measuring prism. ... the pharmaceutical industry, data traceability and documentation are key requirements. It is likely ...
(Date:7/24/2015)... -- Orexigen Therapeutics, Inc. (Nasdaq: OREX ) will ... 2015 on Thursday, August 6 before the market opens. ... and discuss the financial results in a live webcast ... a.m. Pacific Time). The live call may ... or (847) 413-3238 (international), participant code 40327229. The webcast ...
(Date:7/23/2015)... 23, 2015 ImmunoCellular Therapeutics, Ltd. ("ImmunoCellular") (NYSE MKT: ... for the second quarter 2015 on Thursday, August 6, 2015. ... webcast on that day at 5:00 pm ET to discuss ... update. The call will be hosted by Andrew Gengos ... , , LIVE CALL: , , ,  (877) ...
Breaking Biology Technology:Brain & Behavior Research Foundation Names Winners of Klerman-Freedman Prizes for Exceptional Research 2Brain & Behavior Research Foundation Names Winners of Klerman-Freedman Prizes for Exceptional Research 3Brain & Behavior Research Foundation Names Winners of Klerman-Freedman Prizes for Exceptional Research 4Brain & Behavior Research Foundation Names Winners of Klerman-Freedman Prizes for Exceptional Research 5Brain & Behavior Research Foundation Names Winners of Klerman-Freedman Prizes for Exceptional Research 6It’s T-Time for Refractometers: On-site Temperature Check Now Available 2ImmunoCellular Therapeutics to Report Second Quarter 2015 Financial Results on August 6, 2015 2
... Improves Physician Efficiency and Reduces Duplicative and Unnecessary ... ... Nov. 26 RSNA Booth #5924 -- Compressus Inc.,and Advanced ... announced today that ARC has improved physician,efficiency and patient safety, ...
... $5.2 Million ... for Q3 of 2006, -- Gross profit increases 102% to $2.6 Million for Q3 of ... Million for Q3 ... Equity, BEIJING, Nov. 23 /Xinhua-PRNewswire/ -- China Biopharmaceuticals,Holdings, Inc. (OTC Bulletin Board: CHBP), a ...
... PARIS, Nov. 23 Bristol-,Myers Squibb Company (NYSE: ... today,that they are terminating their license agreement for ... for the treatment of,advanced or metastatic bladder cancer ... Fabre Medicament and licensed to Bristol-Myers Squibb,in 2004 ...
Cached Biology Technology:Diagnostic Turnaround Improved by 600% Through Interoperability 2Diagnostic Turnaround Improved by 600% Through Interoperability 3Diagnostic Turnaround Improved by 600% Through Interoperability 4China Biopharmaceuticals Holdings Announces Third Quarter 2007 Financial Results 2China Biopharmaceuticals Holdings Announces Third Quarter 2007 Financial Results 3China Biopharmaceuticals Holdings Announces Third Quarter 2007 Financial Results 4China Biopharmaceuticals Holdings Announces Third Quarter 2007 Financial Results 5China Biopharmaceuticals Holdings Announces Third Quarter 2007 Financial Results 6China Biopharmaceuticals Holdings Announces Third Quarter 2007 Financial Results 7
... System Chemistry Analyzer for General Chemistries, Specialty Chemistries, QC, Reagent Development, Pharmaceutical Development, Physician Owned ... over 30 user-selectable wavelengths. ... ... ...
... for Mac OS X provides the most ... protein sequence analysis and molecular biology data ... Vector NTI Suite you can store manage ... types of molecular biology data all within ...
... Multiple solvents can be used, such as water, benzene, formamide, nitrobenzene, cyclohexane, 1,4-dioxane, p-xylene, dimethyl ... * Range: 12-position switchable: ... ... ...
rat Leptin...
Biology Products: